Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23605 and AO353_23610 overlap by 1 nucleotides AO353_23610 and AO353_23615 overlap by 4 nucleotides AO353_23615 and AO353_23620 are separated by 44 nucleotides AO353_23620 and AO353_23625 overlap by 4 nucleotides
AO353_23605: AO353_23605 - NAD kinase, at 5,168,184 to 5,169,074
_23605
AO353_23610: AO353_23610 - serine/threonine protein phosphatase, at 5,169,074 to 5,170,045
_23610
AO353_23615: AO353_23615 - peptidase S54, at 5,170,042 to 5,170,917
_23615
AO353_23620: AO353_23620 - hypothetical protein, at 5,170,962 to 5,171,222
_23620
AO353_23625: AO353_23625 - hypothetical protein, at 5,171,219 to 5,172,049
_23625
Position (kb)
5170
5171 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5169.091 kb on + strand at 5169.092 kb on - strand at 5169.092 kb on - strand at 5169.095 kb on + strand at 5169.095 kb on + strand at 5169.095 kb on + strand at 5169.095 kb on + strand at 5169.368 kb on + strand, within AO353_23610 at 5169.368 kb on + strand, within AO353_23610 at 5169.368 kb on + strand, within AO353_23610 at 5169.368 kb on + strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.369 kb on - strand, within AO353_23610 at 5169.470 kb on + strand, within AO353_23610 at 5169.470 kb on + strand, within AO353_23610 at 5169.471 kb on - strand, within AO353_23610 at 5169.560 kb on + strand, within AO353_23610 at 5169.560 kb on + strand, within AO353_23610 at 5169.561 kb on - strand, within AO353_23610 at 5169.561 kb on - strand, within AO353_23610 at 5169.561 kb on - strand, within AO353_23610 at 5169.752 kb on + strand at 5169.752 kb on + strand, within AO353_23610 at 5169.752 kb on + strand, within AO353_23610 at 5169.753 kb on - strand, within AO353_23610 at 5169.753 kb on - strand at 5169.829 kb on + strand, within AO353_23610 at 5169.829 kb on + strand, within AO353_23610 at 5169.843 kb on - strand, within AO353_23610 at 5169.878 kb on + strand, within AO353_23610 at 5169.878 kb on + strand, within AO353_23610 at 5169.932 kb on + strand, within AO353_23610 at 5169.932 kb on + strand, within AO353_23610 at 5169.932 kb on + strand, within AO353_23610 at 5169.947 kb on + strand, within AO353_23610 at 5170.061 kb on + strand at 5170.061 kb on + strand at 5170.061 kb on + strand at 5170.062 kb on - strand at 5170.062 kb on - strand at 5170.201 kb on + strand, within AO353_23615 at 5170.420 kb on + strand, within AO353_23615 at 5170.420 kb on + strand, within AO353_23615 at 5170.421 kb on - strand, within AO353_23615 at 5170.621 kb on + strand, within AO353_23615 at 5170.622 kb on - strand, within AO353_23615 at 5170.622 kb on - strand at 5170.630 kb on + strand, within AO353_23615 at 5170.631 kb on - strand, within AO353_23615 at 5170.633 kb on + strand, within AO353_23615 at 5170.636 kb on + strand, within AO353_23615 at 5170.706 kb on - strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.785 kb on + strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.786 kb on - strand, within AO353_23615 at 5170.888 kb on + strand at 5170.888 kb on + strand at 5170.889 kb on - strand at 5170.889 kb on - strand at 5170.889 kb on - strand at 5170.889 kb on - strand at 5170.889 kb on - strand at 5170.927 kb on + strand at 5171.007 kb on + strand, within AO353_23620 at 5171.008 kb on - strand, within AO353_23620 at 5171.008 kb on - strand, within AO353_23620 at 5171.181 kb on - strand, within AO353_23620 at 5171.181 kb on - strand, within AO353_23620 at 5171.192 kb on + strand at 5171.192 kb on + strand, within AO353_23620 at 5171.286 kb on - strand at 5171.286 kb on - strand at 5171.354 kb on + strand, within AO353_23625 at 5171.354 kb on + strand, within AO353_23625 at 5171.355 kb on - strand, within AO353_23625 at 5171.417 kb on + strand, within AO353_23625 at 5171.487 kb on - strand, within AO353_23625 at 5171.487 kb on - strand, within AO353_23625 at 5171.552 kb on + strand, within AO353_23625 at 5171.552 kb on + strand, within AO353_23625 at 5171.553 kb on - strand, within AO353_23625 at 5171.553 kb on - strand, within AO353_23625 at 5171.553 kb on - strand, within AO353_23625 at 5171.596 kb on + strand, within AO353_23625 at 5171.596 kb on + strand, within AO353_23625 at 5171.856 kb on + strand, within AO353_23625 at 5171.856 kb on + strand, within AO353_23625
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 5,169,091 + +0.1 5,169,092 - +1.7 5,169,092 - +1.7 5,169,095 + +0.7 5,169,095 + +0.4 5,169,095 + -0.1 5,169,095 + +0.5 5,169,368 + AO353_23610 0.30 +0.7 5,169,368 + AO353_23610 0.30 +0.0 5,169,368 + AO353_23610 0.30 -0.6 5,169,368 + AO353_23610 0.30 +0.1 5,169,369 - AO353_23610 0.30 -0.6 5,169,369 - AO353_23610 0.30 +0.7 5,169,369 - AO353_23610 0.30 +2.4 5,169,369 - AO353_23610 0.30 +0.7 5,169,369 - AO353_23610 0.30 -1.1 5,169,470 + AO353_23610 0.41 -0.1 5,169,470 + AO353_23610 0.41 +0.0 5,169,471 - AO353_23610 0.41 +1.1 5,169,560 + AO353_23610 0.50 -0.9 5,169,560 + AO353_23610 0.50 +0.2 5,169,561 - AO353_23610 0.50 -0.9 5,169,561 - AO353_23610 0.50 +1.1 5,169,561 - AO353_23610 0.50 +1.1 5,169,752 + -0.3 5,169,752 + AO353_23610 0.70 +0.7 5,169,752 + AO353_23610 0.70 -0.5 5,169,753 - AO353_23610 0.70 +0.7 5,169,753 - +0.1 5,169,829 + AO353_23610 0.78 +0.3 5,169,829 + AO353_23610 0.78 +0.7 5,169,843 - AO353_23610 0.79 -0.1 5,169,878 + AO353_23610 0.83 -0.9 5,169,878 + AO353_23610 0.83 +0.5 5,169,932 + AO353_23610 0.88 -0.5 5,169,932 + AO353_23610 0.88 -0.0 5,169,932 + AO353_23610 0.88 -0.5 5,169,947 + AO353_23610 0.90 -1.3 5,170,061 + -0.6 5,170,061 + -0.9 5,170,061 + -0.2 5,170,062 - +0.1 5,170,062 - +1.7 5,170,201 + AO353_23615 0.18 +0.0 5,170,420 + AO353_23615 0.43 +1.4 5,170,420 + AO353_23615 0.43 -0.1 5,170,421 - AO353_23615 0.43 +0.8 5,170,621 + AO353_23615 0.66 +0.2 5,170,622 - AO353_23615 0.66 +2.1 5,170,622 - +1.7 5,170,630 + AO353_23615 0.67 -0.6 5,170,631 - AO353_23615 0.67 +0.7 5,170,633 + AO353_23615 0.67 -2.6 5,170,636 + AO353_23615 0.68 +0.7 5,170,706 - AO353_23615 0.76 +1.2 5,170,785 + AO353_23615 0.85 -0.2 5,170,785 + AO353_23615 0.85 -1.6 5,170,785 + AO353_23615 0.85 +2.1 5,170,785 + AO353_23615 0.85 +0.7 5,170,785 + AO353_23615 0.85 -0.7 5,170,786 - AO353_23615 0.85 +2.3 5,170,786 - AO353_23615 0.85 +1.6 5,170,786 - AO353_23615 0.85 +0.5 5,170,786 - AO353_23615 0.85 +1.8 5,170,786 - AO353_23615 0.85 +3.6 5,170,786 - AO353_23615 0.85 +0.7 5,170,786 - AO353_23615 0.85 +2.4 5,170,888 + +0.2 5,170,888 + +1.2 5,170,889 - -0.3 5,170,889 - +3.1 5,170,889 - -1.9 5,170,889 - +1.3 5,170,889 - +1.7 5,170,927 + +0.9 5,171,007 + AO353_23620 0.17 -2.3 5,171,008 - AO353_23620 0.18 +3.9 5,171,008 - AO353_23620 0.18 +0.5 5,171,181 - AO353_23620 0.84 +1.3 5,171,181 - AO353_23620 0.84 +0.7 5,171,192 + +0.1 5,171,192 + AO353_23620 0.88 -0.5 5,171,286 - +0.2 5,171,286 - -0.1 5,171,354 + AO353_23625 0.16 -0.3 5,171,354 + AO353_23625 0.16 -2.4 5,171,355 - AO353_23625 0.16 -1.6 5,171,417 + AO353_23625 0.24 +0.5 5,171,487 - AO353_23625 0.32 -0.3 5,171,487 - AO353_23625 0.32 -0.9 5,171,552 + AO353_23625 0.40 +0.7 5,171,552 + AO353_23625 0.40 -1.3 5,171,553 - AO353_23625 0.40 -2.2 5,171,553 - AO353_23625 0.40 +0.9 5,171,553 - AO353_23625 0.40 +0.4 5,171,596 + AO353_23625 0.45 +0.4 5,171,596 + AO353_23625 0.45 +0.9 5,171,856 + AO353_23625 0.77 -0.3 5,171,856 + AO353_23625 0.77 -0.9
Or see this region's nucleotide sequence