Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23405 and AO353_23410 are separated by 69 nucleotides AO353_23410 and AO353_23415 are separated by 276 nucleotides AO353_23415 and AO353_23420 are separated by 10 nucleotides AO353_23420 and AO353_23425 are separated by 37 nucleotides
AO353_23405: AO353_23405 - hypothetical protein, at 5,129,162 to 5,129,362
_23405
AO353_23410: AO353_23410 - hypothetical protein, at 5,129,432 to 5,129,716
_23410
AO353_23415: AO353_23415 - lysine transporter LysE, at 5,129,993 to 5,130,580
_23415
AO353_23420: AO353_23420 - AraC family transcriptional regulator, at 5,130,591 to 5,131,424
_23420
AO353_23425: AO353_23425 - 3-demethylubiquinone-9 3-methyltransferase, at 5,131,462 to 5,131,965
_23425
Position (kb) 5129
5130
5131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5129.003 kb on - strand at 5129.036 kb on + strand at 5129.081 kb on + strand at 5129.081 kb on + strand at 5129.082 kb on - strand at 5129.092 kb on + strand at 5129.093 kb on - strand at 5129.105 kb on + strand at 5129.105 kb on + strand at 5129.106 kb on - strand at 5129.106 kb on - strand at 5129.278 kb on + strand, within AO353_23405 at 5129.333 kb on + strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.334 kb on - strand, within AO353_23405 at 5129.384 kb on + strand at 5129.433 kb on + strand at 5129.433 kb on + strand at 5129.439 kb on + strand at 5129.440 kb on - strand at 5129.440 kb on - strand at 5129.549 kb on - strand, within AO353_23410 at 5129.650 kb on + strand, within AO353_23410 at 5129.650 kb on + strand, within AO353_23410 at 5129.651 kb on - strand, within AO353_23410 at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.701 kb on + strand at 5129.702 kb on - strand at 5129.702 kb on - strand at 5129.702 kb on - strand at 5129.849 kb on + strand at 5129.849 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.852 kb on + strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.853 kb on - strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.925 kb on + strand at 5129.926 kb on - strand at 5129.933 kb on + strand at 5129.933 kb on + strand at 5129.934 kb on - strand at 5129.934 kb on - strand at 5129.948 kb on - strand at 5129.948 kb on - strand at 5129.975 kb on + strand at 5129.976 kb on - strand at 5129.976 kb on - strand at 5130.114 kb on + strand, within AO353_23415 at 5130.114 kb on + strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.115 kb on - strand, within AO353_23415 at 5130.180 kb on + strand, within AO353_23415 at 5130.314 kb on + strand, within AO353_23415 at 5130.314 kb on + strand, within AO353_23415 at 5130.337 kb on - strand, within AO353_23415 at 5130.337 kb on - strand, within AO353_23415 at 5130.429 kb on - strand, within AO353_23415 at 5130.443 kb on + strand, within AO353_23415 at 5130.444 kb on - strand, within AO353_23415 at 5130.444 kb on - strand, within AO353_23415 at 5130.468 kb on - strand, within AO353_23415 at 5130.491 kb on + strand, within AO353_23415 at 5130.524 kb on - strand at 5130.578 kb on + strand at 5130.578 kb on + strand at 5130.578 kb on + strand at 5130.578 kb on + strand at 5130.579 kb on - strand at 5130.868 kb on + strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5130.869 kb on - strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.005 kb on + strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.006 kb on - strand, within AO353_23420 at 5131.012 kb on + strand, within AO353_23420 at 5131.012 kb on + strand, within AO353_23420 at 5131.012 kb on + strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.013 kb on - strand, within AO353_23420 at 5131.031 kb on + strand, within AO353_23420 at 5131.032 kb on - strand, within AO353_23420 at 5131.130 kb on + strand, within AO353_23420 at 5131.138 kb on + strand, within AO353_23420 at 5131.138 kb on + strand, within AO353_23420 at 5131.138 kb on + strand, within AO353_23420 at 5131.139 kb on - strand, within AO353_23420 at 5131.139 kb on - strand, within AO353_23420 at 5131.255 kb on + strand, within AO353_23420 at 5131.255 kb on + strand, within AO353_23420 at 5131.255 kb on + strand, within AO353_23420 at 5131.255 kb on + strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.256 kb on - strand, within AO353_23420 at 5131.316 kb on - strand, within AO353_23420 at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.460 kb on + strand at 5131.461 kb on - strand at 5131.461 kb on - strand at 5131.461 kb on - strand at 5131.461 kb on - strand at 5131.461 kb on - strand at 5131.463 kb on + strand at 5131.463 kb on + strand at 5131.463 kb on + strand at 5131.463 kb on + strand at 5131.463 kb on + strand at 5131.464 kb on - strand at 5131.464 kb on - strand at 5131.464 kb on - strand at 5131.464 kb on - strand at 5131.464 kb on - strand at 5131.464 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 5,129,003 - +0.7 5,129,036 + -0.7 5,129,081 + -0.4 5,129,081 + +0.7 5,129,082 - +0.1 5,129,092 + +0.1 5,129,093 - -0.9 5,129,105 + -0.1 5,129,105 + -0.8 5,129,106 - +0.5 5,129,106 - -1.6 5,129,278 + AO353_23405 0.58 -0.5 5,129,333 + AO353_23405 0.85 -0.9 5,129,334 - AO353_23405 0.86 -0.8 5,129,334 - AO353_23405 0.86 -0.9 5,129,334 - AO353_23405 0.86 +0.2 5,129,334 - AO353_23405 0.86 +1.7 5,129,384 + +1.4 5,129,433 + -1.6 5,129,433 + -0.0 5,129,439 + +0.4 5,129,440 - -0.5 5,129,440 - -0.7 5,129,549 - AO353_23410 0.41 +1.4 5,129,650 + AO353_23410 0.76 -0.3 5,129,650 + AO353_23410 0.76 -0.9 5,129,651 - AO353_23410 0.77 +0.1 5,129,701 + -2.5 5,129,701 + +0.1 5,129,701 + +1.0 5,129,701 + +0.7 5,129,701 + +0.5 5,129,701 + +0.2 5,129,701 + -1.6 5,129,702 - -0.6 5,129,702 - +0.5 5,129,702 - +0.0 5,129,849 + +1.3 5,129,849 + -1.0 5,129,852 + -3.2 5,129,852 + -0.1 5,129,852 + -0.4 5,129,852 + -0.2 5,129,852 + +0.7 5,129,853 - -1.4 5,129,853 - +0.7 5,129,853 - -0.0 5,129,853 - +0.4 5,129,853 - +0.1 5,129,853 - +0.3 5,129,925 + -0.3 5,129,925 + +0.7 5,129,925 + +0.3 5,129,925 + -0.5 5,129,925 + +0.4 5,129,925 + +0.3 5,129,926 - -0.3 5,129,933 + +0.7 5,129,933 + -0.0 5,129,934 - +0.2 5,129,934 - -1.3 5,129,948 - +0.4 5,129,948 - +0.3 5,129,975 + -0.6 5,129,976 - -2.1 5,129,976 - -0.3 5,130,114 + AO353_23415 0.21 -1.7 5,130,114 + AO353_23415 0.21 +0.7 5,130,115 - AO353_23415 0.21 -1.3 5,130,115 - AO353_23415 0.21 +0.2 5,130,115 - AO353_23415 0.21 +0.7 5,130,180 + AO353_23415 0.32 -2.3 5,130,314 + AO353_23415 0.55 -1.2 5,130,314 + AO353_23415 0.55 -1.3 5,130,337 - AO353_23415 0.59 +0.7 5,130,337 - AO353_23415 0.59 -1.2 5,130,429 - AO353_23415 0.74 -1.6 5,130,443 + AO353_23415 0.77 +0.6 5,130,444 - AO353_23415 0.77 -1.5 5,130,444 - AO353_23415 0.77 -0.7 5,130,468 - AO353_23415 0.81 +0.2 5,130,491 + AO353_23415 0.85 +0.1 5,130,524 - -0.6 5,130,578 + +0.1 5,130,578 + -1.2 5,130,578 + -1.2 5,130,578 + -0.3 5,130,579 - -0.0 5,130,868 + AO353_23420 0.33 -2.5 5,130,869 - AO353_23420 0.33 -2.5 5,130,869 - AO353_23420 0.33 -1.5 5,130,869 - AO353_23420 0.33 -1.3 5,130,869 - AO353_23420 0.33 +1.1 5,130,869 - AO353_23420 0.33 +0.3 5,130,869 - AO353_23420 0.33 +2.2 5,131,005 + AO353_23420 0.50 +0.7 5,131,005 + AO353_23420 0.50 -0.6 5,131,005 + AO353_23420 0.50 -0.0 5,131,005 + AO353_23420 0.50 -0.3 5,131,005 + AO353_23420 0.50 +3.5 5,131,005 + AO353_23420 0.50 +0.7 5,131,005 + AO353_23420 0.50 -0.0 5,131,005 + AO353_23420 0.50 -1.4 5,131,006 - AO353_23420 0.50 -0.9 5,131,006 - AO353_23420 0.50 -1.2 5,131,006 - AO353_23420 0.50 +0.3 5,131,006 - AO353_23420 0.50 -0.1 5,131,006 - AO353_23420 0.50 -0.4 5,131,006 - AO353_23420 0.50 +0.4 5,131,006 - AO353_23420 0.50 -0.6 5,131,006 - AO353_23420 0.50 -2.5 5,131,006 - AO353_23420 0.50 -0.3 5,131,006 - AO353_23420 0.50 -0.3 5,131,012 + AO353_23420 0.50 -1.1 5,131,012 + AO353_23420 0.50 -1.4 5,131,012 + AO353_23420 0.50 +0.3 5,131,013 - AO353_23420 0.51 +0.4 5,131,013 - AO353_23420 0.51 -0.3 5,131,013 - AO353_23420 0.51 +0.2 5,131,013 - AO353_23420 0.51 -0.9 5,131,013 - AO353_23420 0.51 +0.9 5,131,013 - AO353_23420 0.51 -2.3 5,131,031 + AO353_23420 0.53 +0.4 5,131,032 - AO353_23420 0.53 +0.3 5,131,130 + AO353_23420 0.65 -0.1 5,131,138 + AO353_23420 0.66 +0.2 5,131,138 + AO353_23420 0.66 -0.2 5,131,138 + AO353_23420 0.66 +0.7 5,131,139 - AO353_23420 0.66 -0.1 5,131,139 - AO353_23420 0.66 +0.7 5,131,255 + AO353_23420 0.80 -0.5 5,131,255 + AO353_23420 0.80 -0.1 5,131,255 + AO353_23420 0.80 +0.2 5,131,255 + AO353_23420 0.80 -0.6 5,131,256 - AO353_23420 0.80 -1.3 5,131,256 - AO353_23420 0.80 -0.7 5,131,256 - AO353_23420 0.80 -0.3 5,131,256 - AO353_23420 0.80 +0.7 5,131,256 - AO353_23420 0.80 -2.1 5,131,316 - AO353_23420 0.87 -1.3 5,131,460 + +0.3 5,131,460 + -1.1 5,131,460 + -1.0 5,131,460 + +0.1 5,131,460 + -0.3 5,131,460 + -1.8 5,131,460 + +0.1 5,131,460 + -0.5 5,131,460 + +1.0 5,131,461 - -1.3 5,131,461 - -0.3 5,131,461 - -1.9 5,131,461 - +1.1 5,131,461 - +0.1 5,131,463 + +0.2 5,131,463 + +0.3 5,131,463 + -2.1 5,131,463 + -0.8 5,131,463 + +1.0 5,131,464 - +1.4 5,131,464 - +0.4 5,131,464 - -0.6 5,131,464 - -2.8 5,131,464 - +1.6 5,131,464 - +1.4
Or see this region's nucleotide sequence