Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02225 and AO353_02230 are separated by 245 nucleotides AO353_02230 and AO353_02235 are separated by 4 nucleotides AO353_02235 and AO353_02240 are separated by 100 nucleotides AO353_02240 and AO353_02245 overlap by 4 nucleotides
AO353_02225: AO353_02225 - LuxR family transcriptional regulator, at 477,362 to 477,595
_02225
AO353_02230: AO353_02230 - haloacid dehalogenase, at 477,841 to 478,524
_02230
AO353_02235: AO353_02235 - acireductone dioxygenase, at 478,529 to 479,074
_02235
AO353_02240: AO353_02240 - methylthioribulose-1-phosphate dehydratase, at 479,175 to 479,795
_02240
AO353_02245: AO353_02245 - MFS transporter, at 479,792 to 480,937
_02245
Position (kb) 478
479
480 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 477.599 kb on + strand at 477.599 kb on + strand at 477.599 kb on + strand at 477.599 kb on + strand at 477.652 kb on - strand at 477.665 kb on + strand at 477.665 kb on + strand at 477.680 kb on + strand at 477.680 kb on + strand at 477.680 kb on + strand at 477.680 kb on + strand at 477.680 kb on + strand at 477.681 kb on - strand at 477.681 kb on - strand at 477.681 kb on - strand at 477.696 kb on + strand at 477.696 kb on + strand at 477.696 kb on + strand at 477.696 kb on + strand at 477.696 kb on + strand at 477.697 kb on - strand at 477.697 kb on - strand at 477.697 kb on - strand at 477.697 kb on - strand at 477.704 kb on + strand at 477.704 kb on + strand at 477.704 kb on + strand at 477.704 kb on + strand at 477.705 kb on - strand at 477.705 kb on - strand at 477.735 kb on + strand at 477.735 kb on + strand at 477.735 kb on + strand at 477.736 kb on - strand at 477.754 kb on - strand at 477.754 kb on - strand at 477.754 kb on - strand at 477.762 kb on + strand at 477.763 kb on - strand at 477.787 kb on + strand at 477.787 kb on + strand at 477.787 kb on + strand at 477.788 kb on - strand at 477.788 kb on - strand at 477.788 kb on - strand at 477.788 kb on - strand at 477.788 kb on - strand at 477.788 kb on - strand at 478.707 kb on + strand, within AO353_02235 at 478.708 kb on - strand, within AO353_02235 at 478.708 kb on - strand, within AO353_02235 at 478.708 kb on - strand, within AO353_02235 at 478.708 kb on - strand, within AO353_02235 at 478.714 kb on - strand, within AO353_02235 at 478.714 kb on - strand, within AO353_02235 at 478.714 kb on - strand, within AO353_02235 at 478.714 kb on - strand, within AO353_02235 at 478.714 kb on - strand, within AO353_02235 at 478.714 kb on - strand, within AO353_02235 at 478.733 kb on - strand, within AO353_02235 at 478.838 kb on - strand, within AO353_02235 at 478.863 kb on + strand, within AO353_02235 at 478.864 kb on - strand, within AO353_02235 at 478.864 kb on - strand, within AO353_02235 at 478.864 kb on - strand, within AO353_02235 at 478.864 kb on - strand, within AO353_02235 at 478.864 kb on - strand, within AO353_02235 at 479.115 kb on - strand at 479.163 kb on + strand at 479.163 kb on + strand at 479.169 kb on + strand at 479.169 kb on + strand at 479.169 kb on + strand at 479.169 kb on + strand at 479.170 kb on - strand at 479.170 kb on - strand at 479.170 kb on - strand at 479.170 kb on - strand at 479.275 kb on + strand, within AO353_02240 at 479.275 kb on + strand, within AO353_02240 at 479.275 kb on + strand, within AO353_02240 at 479.275 kb on + strand, within AO353_02240 at 479.276 kb on - strand, within AO353_02240 at 479.276 kb on - strand, within AO353_02240 at 479.440 kb on + strand, within AO353_02240 at 479.440 kb on + strand, within AO353_02240 at 479.441 kb on - strand, within AO353_02240 at 479.441 kb on - strand, within AO353_02240 at 479.453 kb on + strand, within AO353_02240 at 479.453 kb on + strand, within AO353_02240 at 479.454 kb on - strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.540 kb on + strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.541 kb on - strand, within AO353_02240 at 479.542 kb on + strand, within AO353_02240 at 479.542 kb on + strand, within AO353_02240 at 479.542 kb on + strand, within AO353_02240 at 479.542 kb on + strand, within AO353_02240 at 479.542 kb on + strand, within AO353_02240 at 479.543 kb on - strand, within AO353_02240 at 479.543 kb on - strand, within AO353_02240 at 479.543 kb on - strand, within AO353_02240 at 479.543 kb on - strand, within AO353_02240 at 479.543 kb on - strand, within AO353_02240 at 479.543 kb on - strand, within AO353_02240 at 479.543 kb on - strand, within AO353_02240 at 479.543 kb on - strand, within AO353_02240 at 479.544 kb on + strand, within AO353_02240 at 479.544 kb on + strand, within AO353_02240 at 479.544 kb on + strand, within AO353_02240 at 479.544 kb on + strand, within AO353_02240 at 479.544 kb on + strand, within AO353_02240 at 479.544 kb on + strand, within AO353_02240 at 479.544 kb on + strand at 479.545 kb on - strand, within AO353_02240 at 479.545 kb on - strand, within AO353_02240 at 479.545 kb on - strand, within AO353_02240 at 479.545 kb on - strand, within AO353_02240 at 479.609 kb on + strand, within AO353_02240 at 479.609 kb on + strand, within AO353_02240 at 479.609 kb on + strand, within AO353_02240 at 479.609 kb on + strand, within AO353_02240 at 479.610 kb on - strand, within AO353_02240 at 479.610 kb on - strand, within AO353_02240 at 479.610 kb on - strand, within AO353_02240 at 479.610 kb on - strand, within AO353_02240 at 479.701 kb on + strand, within AO353_02240 at 479.701 kb on + strand, within AO353_02240 at 479.702 kb on - strand, within AO353_02240 at 479.702 kb on - strand, within AO353_02240 at 479.702 kb on - strand, within AO353_02240 at 479.702 kb on - strand, within AO353_02240 at 479.702 kb on - strand, within AO353_02240 at 479.702 kb on - strand, within AO353_02240 at 479.702 kb on - strand, within AO353_02240 at 479.702 kb on - strand, within AO353_02240 at 479.737 kb on + strand at 479.737 kb on + strand at 479.737 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.812 kb on + strand at 479.813 kb on - strand at 479.813 kb on - strand at 479.813 kb on - strand at 479.813 kb on - strand at 479.813 kb on - strand at 479.813 kb on - strand at 479.813 kb on - strand at 479.813 kb on - strand at 479.860 kb on + strand at 479.860 kb on + strand at 479.863 kb on + strand at 479.863 kb on + strand at 479.863 kb on + strand at 479.863 kb on + strand at 479.863 kb on + strand at 479.864 kb on - strand at 479.864 kb on - strand at 479.864 kb on - strand at 479.864 kb on - strand at 479.875 kb on + strand at 479.876 kb on - strand at 479.907 kb on + strand, within AO353_02245 at 479.907 kb on + strand, within AO353_02245 at 479.908 kb on - strand, within AO353_02245 at 479.908 kb on - strand, within AO353_02245 at 479.908 kb on - strand, within AO353_02245 at 479.949 kb on + strand, within AO353_02245 at 479.949 kb on + strand, within AO353_02245 at 479.950 kb on - strand, within AO353_02245 at 479.950 kb on - strand, within AO353_02245 at 479.950 kb on - strand, within AO353_02245 at 479.950 kb on - strand, within AO353_02245 at 479.950 kb on - strand, within AO353_02245 at 479.989 kb on + strand, within AO353_02245 at 479.990 kb on - strand, within AO353_02245 at 479.990 kb on - strand, within AO353_02245 at 479.990 kb on - strand, within AO353_02245
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 477,599 + -0.1 477,599 + +1.2 477,599 + +0.5 477,599 + -0.2 477,652 - +1.6 477,665 + -1.7 477,665 + +0.8 477,680 + +0.2 477,680 + -0.7 477,680 + -1.4 477,680 + -0.5 477,680 + -0.6 477,681 - -0.2 477,681 - +0.5 477,681 - +0.6 477,696 + +0.9 477,696 + -0.9 477,696 + -0.6 477,696 + -0.1 477,696 + +0.7 477,697 - -0.1 477,697 - -2.1 477,697 - -0.4 477,697 - -0.7 477,704 + -0.7 477,704 + +0.5 477,704 + -0.8 477,704 + -0.7 477,705 - -0.7 477,705 - +0.2 477,735 + -1.4 477,735 + -0.3 477,735 + -1.9 477,736 - -0.1 477,754 - -0.3 477,754 - -0.1 477,754 - -1.3 477,762 + -1.4 477,763 - +1.6 477,787 + -2.8 477,787 + -0.3 477,787 + -0.5 477,788 - -0.1 477,788 - -0.1 477,788 - -0.4 477,788 - -0.8 477,788 - -1.9 477,788 - +0.2 478,707 + AO353_02235 0.33 -1.1 478,708 - AO353_02235 0.33 -0.3 478,708 - AO353_02235 0.33 +0.3 478,708 - AO353_02235 0.33 -0.1 478,708 - AO353_02235 0.33 -0.8 478,714 - AO353_02235 0.34 -1.2 478,714 - AO353_02235 0.34 -0.1 478,714 - AO353_02235 0.34 -0.1 478,714 - AO353_02235 0.34 -2.0 478,714 - AO353_02235 0.34 -1.7 478,714 - AO353_02235 0.34 -2.4 478,733 - AO353_02235 0.37 -1.5 478,838 - AO353_02235 0.57 -1.1 478,863 + AO353_02235 0.61 -1.9 478,864 - AO353_02235 0.61 +0.5 478,864 - AO353_02235 0.61 -0.4 478,864 - AO353_02235 0.61 -1.8 478,864 - AO353_02235 0.61 -2.1 478,864 - AO353_02235 0.61 -2.3 479,115 - +0.0 479,163 + -1.9 479,163 + +0.4 479,169 + -2.5 479,169 + -1.4 479,169 + +0.6 479,169 + -0.9 479,170 - -0.3 479,170 - -0.3 479,170 - +0.7 479,170 - +0.8 479,275 + AO353_02240 0.16 -0.3 479,275 + AO353_02240 0.16 -0.1 479,275 + AO353_02240 0.16 -1.5 479,275 + AO353_02240 0.16 -1.7 479,276 - AO353_02240 0.16 +1.0 479,276 - AO353_02240 0.16 +1.1 479,440 + AO353_02240 0.43 -0.5 479,440 + AO353_02240 0.43 +2.5 479,441 - AO353_02240 0.43 -0.1 479,441 - AO353_02240 0.43 +0.3 479,453 + AO353_02240 0.45 -0.7 479,453 + AO353_02240 0.45 -0.7 479,454 - AO353_02240 0.45 +0.9 479,540 + AO353_02240 0.59 -2.1 479,540 + AO353_02240 0.59 -1.2 479,540 + AO353_02240 0.59 +1.1 479,540 + AO353_02240 0.59 -1.4 479,540 + AO353_02240 0.59 -1.9 479,540 + AO353_02240 0.59 -0.9 479,540 + AO353_02240 0.59 -1.1 479,540 + AO353_02240 0.59 +1.7 479,540 + AO353_02240 0.59 -1.4 479,540 + AO353_02240 0.59 -1.4 479,540 + AO353_02240 0.59 +0.6 479,541 - AO353_02240 0.59 -2.3 479,541 - AO353_02240 0.59 -0.9 479,541 - +1.9 479,541 - AO353_02240 0.59 +0.0 479,541 - AO353_02240 0.59 +0.2 479,541 - AO353_02240 0.59 -1.5 479,541 - AO353_02240 0.59 +0.4 479,541 - AO353_02240 0.59 +0.4 479,541 - AO353_02240 0.59 +1.5 479,541 - AO353_02240 0.59 +0.7 479,541 - AO353_02240 0.59 +0.9 479,542 + AO353_02240 0.59 +0.9 479,542 + AO353_02240 0.59 +0.9 479,542 + AO353_02240 0.59 -0.4 479,542 + AO353_02240 0.59 +0.9 479,542 + AO353_02240 0.59 -0.0 479,543 - AO353_02240 0.59 -1.1 479,543 - AO353_02240 0.59 -0.1 479,543 - AO353_02240 0.59 -1.1 479,543 - AO353_02240 0.59 -0.4 479,543 - AO353_02240 0.59 +0.3 479,543 - AO353_02240 0.59 -2.1 479,543 - AO353_02240 0.59 -1.4 479,543 - AO353_02240 0.59 +0.0 479,544 + AO353_02240 0.59 +1.7 479,544 + AO353_02240 0.59 -1.4 479,544 + AO353_02240 0.59 +0.8 479,544 + AO353_02240 0.59 -0.0 479,544 + AO353_02240 0.59 -0.1 479,544 + AO353_02240 0.59 +0.9 479,544 + +0.9 479,545 - AO353_02240 0.60 +0.5 479,545 - AO353_02240 0.60 -0.1 479,545 - AO353_02240 0.60 -0.3 479,545 - AO353_02240 0.60 -0.5 479,609 + AO353_02240 0.70 -2.1 479,609 + AO353_02240 0.70 -0.2 479,609 + AO353_02240 0.70 -0.8 479,609 + AO353_02240 0.70 -0.5 479,610 - AO353_02240 0.70 -3.0 479,610 - AO353_02240 0.70 -0.7 479,610 - AO353_02240 0.70 -1.2 479,610 - AO353_02240 0.70 +0.9 479,701 + AO353_02240 0.85 -1.2 479,701 + AO353_02240 0.85 -3.2 479,702 - AO353_02240 0.85 -2.1 479,702 - AO353_02240 0.85 +0.9 479,702 - AO353_02240 0.85 -0.4 479,702 - AO353_02240 0.85 +1.2 479,702 - AO353_02240 0.85 -1.4 479,702 - AO353_02240 0.85 +0.3 479,702 - AO353_02240 0.85 +0.3 479,702 - AO353_02240 0.85 +1.2 479,737 + +0.5 479,737 + -1.1 479,737 + -0.1 479,812 + -1.4 479,812 + +0.3 479,812 + +0.7 479,812 + -0.4 479,812 + +0.6 479,812 + -1.4 479,812 + +2.9 479,812 + -1.1 479,812 + -1.2 479,812 + -0.1 479,812 + -0.8 479,812 + -0.7 479,812 + +1.9 479,813 - -1.5 479,813 - -1.9 479,813 - +0.3 479,813 - +0.8 479,813 - +1.9 479,813 - +0.3 479,813 - +1.2 479,813 - -0.1 479,860 + -0.1 479,860 + -1.1 479,863 + -0.5 479,863 + -0.4 479,863 + +0.1 479,863 + -1.7 479,863 + -1.5 479,864 - +0.7 479,864 - +0.2 479,864 - +0.5 479,864 - +0.3 479,875 + -0.1 479,876 - +0.2 479,907 + AO353_02245 0.10 +0.3 479,907 + AO353_02245 0.10 +0.9 479,908 - AO353_02245 0.10 -0.3 479,908 - AO353_02245 0.10 +0.5 479,908 - AO353_02245 0.10 -0.2 479,949 + AO353_02245 0.14 +0.4 479,949 + AO353_02245 0.14 -0.5 479,950 - AO353_02245 0.14 +0.4 479,950 - AO353_02245 0.14 +0.9 479,950 - AO353_02245 0.14 +2.3 479,950 - AO353_02245 0.14 -0.1 479,950 - AO353_02245 0.14 +0.9 479,989 + AO353_02245 0.17 +0.2 479,990 - AO353_02245 0.17 -0.1 479,990 - AO353_02245 0.17 +1.3 479,990 - AO353_02245 0.17 +0.0
Or see this region's nucleotide sequence