Experiment: benzoic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and Shewana3_1610 are separated by 47 nucleotides Shewana3_1610 and Shewana3_R0079 are separated by 210 nucleotides Shewana3_R0079 and Shewana3_R0080 are separated by 20 nucleotides Shewana3_R0080 and Shewana3_R0081 are separated by 48 nucleotides Shewana3_R0081 and Shewana3_R0082 are separated by 131 nucleotides
Shewana3_1609: uvrC - excinuclease ABC subunit C (RefSeq), at 1,889,881 to 1,891,710
uvrC
Shewana3_1610: Shewana3_1610 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (RefSeq), at 1,891,758 to 1,892,306
_1610
Shewana3_R0079: Shewana3_R0079 - tRNA-Gly (RefSeq), at 1,892,517 to 1,892,592
_R0079
Shewana3_R0080: Shewana3_R0080 - tRNA-Cys (RefSeq), at 1,892,613 to 1,892,686
_R0080
Shewana3_R0081: Shewana3_R0081 - tRNA-Leu (RefSeq), at 1,892,735 to 1,892,821
_R0081
Shewana3_R0082: Shewana3_R0082 - tRNA-Leu (RefSeq), at 1,892,953 to 1,893,039
_R0082
Position (kb) 1892
1893 Strain fitness (log2 ratio) -1
0
1
2 at 1892.555 kb on + strand, within Shewana3_R0079 at 1892.569 kb on + strand, within Shewana3_R0079 at 1893.016 kb on + strand, within Shewana3_R0082 at 1893.282 kb on + strand at 1893.559 kb on + strand at 1893.585 kb on - strand at 1893.606 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.4 mg/ml remove 1,892,555 + Shewana3_R0079 0.50 +0.3 1,892,569 + Shewana3_R0079 0.68 -0.2 1,893,016 + Shewana3_R0082 0.72 -0.2 1,893,282 + +1.0 1,893,559 + +1.0 1,893,585 - +2.0 1,893,606 + +1.0
Or see this region's nucleotide sequence