Experiment: benzoic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1010 and Shewana3_1011 are separated by 167 nucleotides Shewana3_1011 and Shewana3_1012 are separated by 139 nucleotides Shewana3_1012 and Shewana3_R0031 are separated by 207 nucleotides Shewana3_R0031 and Shewana3_R0032 are separated by 54 nucleotides Shewana3_R0032 and Shewana3_R0033 are separated by 47 nucleotides Shewana3_R0033 and Shewana3_R0034 are separated by 22 nucleotides Shewana3_R0034 and Shewana3_R0035 are separated by 1 nucleotides Shewana3_R0035 and Shewana3_R0036 are separated by 83 nucleotides Shewana3_R0036 and Shewana3_R0037 are separated by 36 nucleotides
Shewana3_1010: Shewana3_1010 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (RefSeq), at 1,180,756 to 1,181,982
_1010
Shewana3_1011: Shewana3_1011 - peptidyl-tRNA hydrolase (RefSeq), at 1,182,150 to 1,182,737
_1011
Shewana3_1012: Shewana3_1012 - GTP-dependent nucleic acid-binding protein EngD (RefSeq), at 1,182,877 to 1,183,968
_1012
Shewana3_R0031: Shewana3_R0031 - tRNA-Met (RefSeq), at 1,184,176 to 1,184,252
_R0031
Shewana3_R0032: Shewana3_R0032 - tRNA-Leu (RefSeq), at 1,184,307 to 1,184,391
_R0032
Shewana3_R0033: Shewana3_R0033 - tRNA-Gln (RefSeq), at 1,184,439 to 1,184,513
_R0033
Shewana3_R0034: Shewana3_R0034 - tRNA-Gln (RefSeq), at 1,184,536 to 1,184,610
_R0034
Shewana3_R0035: Shewana3_R0035 - tRNA-Met (RefSeq), at 1,184,612 to 1,184,688
_R0035
Shewana3_R0036: Shewana3_R0036 - tRNA-Leu (RefSeq), at 1,184,772 to 1,184,856
_R0036
Shewana3_R0037: Shewana3_R0037 - tRNA-Gln (RefSeq), at 1,184,893 to 1,184,967
_R0037
Position (kb)
1182
1183
1184 Strain fitness (log2 ratio)
-2
-1
0
1 at 1182.003 kb on - strand at 1182.689 kb on + strand at 1182.711 kb on + strand at 1182.722 kb on + strand at 1182.740 kb on - strand at 1182.802 kb on + strand at 1182.802 kb on + strand at 1182.810 kb on - strand at 1182.881 kb on - strand at 1183.108 kb on - strand, within Shewana3_1012 at 1183.230 kb on + strand, within Shewana3_1012 at 1183.292 kb on + strand, within Shewana3_1012 at 1183.292 kb on + strand, within Shewana3_1012 at 1183.300 kb on - strand, within Shewana3_1012 at 1183.469 kb on + strand, within Shewana3_1012 at 1183.655 kb on + strand, within Shewana3_1012 at 1183.693 kb on + strand, within Shewana3_1012 at 1183.757 kb on - strand, within Shewana3_1012 at 1183.819 kb on + strand, within Shewana3_1012 at 1183.916 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction benzoic 0.4 mg/ml remove 1,182,003 - +0.1 1,182,689 + -0.2 1,182,711 + -0.6 1,182,722 + +0.0 1,182,740 - -0.3 1,182,802 + -0.3 1,182,802 + -0.4 1,182,810 - -0.6 1,182,881 - -1.1 1,183,108 - Shewana3_1012 0.21 -2.4 1,183,230 + Shewana3_1012 0.32 -0.6 1,183,292 + Shewana3_1012 0.38 -1.4 1,183,292 + Shewana3_1012 0.38 -1.4 1,183,300 - Shewana3_1012 0.39 -2.5 1,183,469 + Shewana3_1012 0.54 -1.1 1,183,655 + Shewana3_1012 0.71 -0.6 1,183,693 + Shewana3_1012 0.75 -0.8 1,183,757 - Shewana3_1012 0.81 +0.0 1,183,819 + Shewana3_1012 0.86 -0.7 1,183,916 + +0.1
Or see this region's nucleotide sequence