Experiment: MoYLS4 with Nitrite 0.125mg/mL + Zinc Sulfate 0.1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0622 and nrfH are separated by 342 nucleotides nrfH and nrfA overlap by 8 nucleotides nrfA and ilvN-1 are separated by 432 nucleotides ilvN-1 and ptB are separated by 3 nucleotides
DVU0622: DVU0622 - sensor histidine kinase/response regulator (TIGR), at 688,231 to 690,729
DVU0622
DVU0624: nrfH - NapC/NirT cytochrome c family protein (TIGR), at 691,072 to 691,551
nrfH
DVU0625: nrfA - cytochrome c nitrite reductase, catalytic subunit NrfA, putative (Shelley Haveman), at 691,544 to 693,118
nrfA
DVU0626: ilvN-1 - acetolactate synthase, small subunit (TIGR), at 693,551 to 694,030
ilvN-1
DVU0627: ptB - phosphotransbutyrylase (TIGR), at 694,034 to 695,065
ptB
Position (kb) 691
692
693
694 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 690.565 kb on + strand at 690.579 kb on + strand at 690.614 kb on - strand at 690.652 kb on + strand at 690.652 kb on + strand at 690.660 kb on - strand at 690.674 kb on + strand at 690.679 kb on + strand at 690.714 kb on + strand at 690.722 kb on - strand at 690.722 kb on - strand at 690.722 kb on - strand at 690.727 kb on - strand at 690.966 kb on - strand at 691.029 kb on + strand at 691.213 kb on - strand, within nrfH at 691.221 kb on - strand, within nrfH at 691.311 kb on + strand, within nrfH at 691.337 kb on - strand, within nrfH at 691.402 kb on + strand, within nrfH at 691.415 kb on + strand, within nrfH at 691.424 kb on - strand, within nrfH at 691.424 kb on - strand, within nrfH at 691.431 kb on + strand, within nrfH at 691.465 kb on + strand, within nrfH at 691.481 kb on - strand, within nrfH at 691.498 kb on + strand, within nrfH at 691.498 kb on + strand, within nrfH at 691.503 kb on + strand, within nrfH at 691.506 kb on - strand at 691.569 kb on - strand at 691.774 kb on - strand, within nrfA at 691.779 kb on - strand, within nrfA at 691.823 kb on + strand, within nrfA at 691.855 kb on + strand, within nrfA at 691.863 kb on - strand, within nrfA at 691.908 kb on - strand, within nrfA at 691.990 kb on + strand, within nrfA at 691.990 kb on + strand, within nrfA at 692.016 kb on + strand, within nrfA at 692.018 kb on + strand, within nrfA at 692.120 kb on + strand, within nrfA at 692.120 kb on + strand, within nrfA at 692.127 kb on + strand, within nrfA at 692.127 kb on + strand, within nrfA at 692.135 kb on - strand, within nrfA at 692.135 kb on - strand, within nrfA at 692.137 kb on - strand, within nrfA at 692.187 kb on - strand, within nrfA at 692.230 kb on - strand, within nrfA at 692.246 kb on + strand, within nrfA at 692.335 kb on + strand, within nrfA at 692.402 kb on - strand, within nrfA at 692.455 kb on + strand, within nrfA at 692.515 kb on - strand, within nrfA at 692.658 kb on + strand, within nrfA at 692.658 kb on + strand, within nrfA at 692.708 kb on + strand, within nrfA at 692.770 kb on - strand, within nrfA at 692.776 kb on - strand, within nrfA at 692.959 kb on + strand, within nrfA at 693.037 kb on - strand at 693.061 kb on + strand at 693.061 kb on + strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.069 kb on - strand at 693.076 kb on + strand at 693.093 kb on + strand at 693.095 kb on + strand at 693.103 kb on - strand at 693.140 kb on + strand at 693.148 kb on - strand at 693.185 kb on + strand at 693.295 kb on + strand at 693.301 kb on - strand at 693.312 kb on + strand at 693.330 kb on - strand at 693.332 kb on + strand at 693.353 kb on + strand at 693.479 kb on + strand at 693.506 kb on - strand at 693.513 kb on + strand at 693.521 kb on - strand at 693.530 kb on + strand at 693.616 kb on - strand, within ilvN-1 at 693.628 kb on - strand, within ilvN-1 at 693.735 kb on - strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.814 kb on + strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.822 kb on - strand, within ilvN-1 at 693.904 kb on + strand, within ilvN-1 at 693.947 kb on + strand, within ilvN-1 at 693.984 kb on + strand at 693.984 kb on + strand at 694.051 kb on - strand at 694.085 kb on - strand at 694.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nitrite 0.125mg/mL + Zinc Sulfate 0.1mM remove 690,565 + -0.1 690,579 + +0.2 690,614 - +0.5 690,652 + -0.2 690,652 + +0.1 690,660 - -0.2 690,674 + +0.4 690,679 + -0.4 690,714 + -0.1 690,722 - -0.2 690,722 - -0.4 690,722 - -1.8 690,727 - +0.4 690,966 - -0.3 691,029 + -1.3 691,213 - nrfH DVU0624 0.29 -5.8 691,221 - nrfH DVU0624 0.31 -3.9 691,311 + nrfH DVU0624 0.50 -4.5 691,337 - nrfH DVU0624 0.55 -4.4 691,402 + nrfH DVU0624 0.69 -4.9 691,415 + nrfH DVU0624 0.71 +1.7 691,424 - nrfH DVU0624 0.73 -5.2 691,424 - nrfH DVU0624 0.73 -4.2 691,431 + nrfH DVU0624 0.75 -3.6 691,465 + nrfH DVU0624 0.82 -2.2 691,481 - nrfH DVU0624 0.85 -5.5 691,498 + nrfH DVU0624 0.89 -3.3 691,498 + nrfH DVU0624 0.89 -1.9 691,503 + nrfH DVU0624 0.90 -1.8 691,506 - -4.8 691,569 - -0.7 691,774 - nrfA DVU0625 0.15 -4.3 691,779 - nrfA DVU0625 0.15 -2.4 691,823 + nrfA DVU0625 0.18 -2.6 691,855 + nrfA DVU0625 0.20 -3.7 691,863 - nrfA DVU0625 0.20 -3.1 691,908 - nrfA DVU0625 0.23 -4.2 691,990 + nrfA DVU0625 0.28 -3.5 691,990 + nrfA DVU0625 0.28 -2.8 692,016 + nrfA DVU0625 0.30 -3.6 692,018 + nrfA DVU0625 0.30 -4.6 692,120 + nrfA DVU0625 0.37 -4.0 692,120 + nrfA DVU0625 0.37 -3.8 692,127 + nrfA DVU0625 0.37 -5.8 692,127 + nrfA DVU0625 0.37 -4.1 692,135 - nrfA DVU0625 0.38 -2.3 692,135 - nrfA DVU0625 0.38 -5.4 692,137 - nrfA DVU0625 0.38 -3.8 692,187 - nrfA DVU0625 0.41 -4.0 692,230 - nrfA DVU0625 0.44 -3.9 692,246 + nrfA DVU0625 0.45 -2.5 692,335 + nrfA DVU0625 0.50 -5.2 692,402 - nrfA DVU0625 0.54 -1.2 692,455 + nrfA DVU0625 0.58 -4.4 692,515 - nrfA DVU0625 0.62 -2.8 692,658 + nrfA DVU0625 0.71 -2.0 692,658 + nrfA DVU0625 0.71 -2.1 692,708 + nrfA DVU0625 0.74 -4.2 692,770 - nrfA DVU0625 0.78 -1.2 692,776 - nrfA DVU0625 0.78 -3.7 692,959 + nrfA DVU0625 0.90 -1.8 693,037 - -2.7 693,061 + -3.4 693,061 + +0.7 693,069 - -4.1 693,069 - -4.0 693,069 - -1.0 693,069 - -3.5 693,069 - -4.0 693,069 - -4.7 693,069 - -0.0 693,076 + -3.5 693,093 + -3.8 693,095 + -4.5 693,103 - -4.4 693,140 + -2.9 693,148 - -4.2 693,185 + -0.3 693,295 + -0.2 693,301 - -0.5 693,312 + -0.1 693,330 - -0.7 693,332 + -0.0 693,353 + -0.5 693,479 + -0.3 693,506 - +0.2 693,513 + +0.3 693,521 - -0.1 693,530 + +1.2 693,616 - ilvN-1 DVU0626 0.14 +0.4 693,628 - ilvN-1 DVU0626 0.16 +0.1 693,735 - ilvN-1 DVU0626 0.38 -0.8 693,814 + ilvN-1 DVU0626 0.55 -0.1 693,814 + ilvN-1 DVU0626 0.55 -0.6 693,814 + ilvN-1 DVU0626 0.55 -0.3 693,822 - ilvN-1 DVU0626 0.56 -0.2 693,822 - ilvN-1 DVU0626 0.56 -0.4 693,822 - ilvN-1 DVU0626 0.56 -0.7 693,822 - ilvN-1 DVU0626 0.56 -0.3 693,822 - ilvN-1 DVU0626 0.56 +0.5 693,822 - ilvN-1 DVU0626 0.56 +0.1 693,822 - ilvN-1 DVU0626 0.56 -0.1 693,904 + ilvN-1 DVU0626 0.74 +0.4 693,947 + ilvN-1 DVU0626 0.82 -0.3 693,984 + -1.1 693,984 + -0.1 694,051 - -0.2 694,085 - -0.0 694,113 + -0.5
Or see this region's nucleotide sequence