Experiment: L-Arginine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1040 and Pf1N1B4_1041 are separated by 22 nucleotides Pf1N1B4_1041 and Pf1N1B4_1042 are separated by 2 nucleotides Pf1N1B4_1042 and Pf1N1B4_1043 are separated by 96 nucleotides Pf1N1B4_1043 and Pf1N1B4_1044 overlap by 8 nucleotides Pf1N1B4_1044 and Pf1N1B4_1045 are separated by 194 nucleotides
Pf1N1B4_1040 - FolM Alternative dihydrofolate reductase 1, at 1,178,800 to 1,179,510
_1040
Pf1N1B4_1041 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 1,179,533 to 1,180,108
_1041
Pf1N1B4_1042 - Dihydroneopterin triphosphate epimerase, at 1,180,111 to 1,180,482
_1042
Pf1N1B4_1043 - FIG00953380: hypothetical protein, at 1,180,579 to 1,180,875
_1043
Pf1N1B4_1044 - Type III effector HopPmaJ, at 1,180,868 to 1,181,206
_1044
Pf1N1B4_1045 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 1,181,401 to 1,182,651
_1045
Position (kb)
1180
1181 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.120 kb on + strand, within Pf1N1B4_1040 at 1179.121 kb on - strand, within Pf1N1B4_1040 at 1179.121 kb on - strand, within Pf1N1B4_1040 at 1179.121 kb on - strand at 1179.338 kb on + strand, within Pf1N1B4_1040 at 1179.338 kb on + strand at 1179.339 kb on - strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.427 kb on + strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.428 kb on - strand, within Pf1N1B4_1040 at 1179.442 kb on + strand at 1179.460 kb on + strand at 1179.460 kb on + strand at 1179.460 kb on + strand at 1179.461 kb on - strand at 1179.461 kb on - strand at 1179.461 kb on - strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.508 kb on + strand at 1179.541 kb on - strand at 1179.573 kb on - strand at 1179.659 kb on + strand, within Pf1N1B4_1041 at 1179.659 kb on + strand at 1179.659 kb on + strand, within Pf1N1B4_1041 at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.660 kb on - strand at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.660 kb on - strand, within Pf1N1B4_1041 at 1179.674 kb on + strand, within Pf1N1B4_1041 at 1179.675 kb on - strand, within Pf1N1B4_1041 at 1179.761 kb on + strand, within Pf1N1B4_1041 at 1179.761 kb on + strand, within Pf1N1B4_1041 at 1179.762 kb on - strand, within Pf1N1B4_1041 at 1179.762 kb on - strand, within Pf1N1B4_1041 at 1179.815 kb on + strand, within Pf1N1B4_1041 at 1179.815 kb on + strand, within Pf1N1B4_1041 at 1179.815 kb on + strand at 1179.815 kb on + strand, within Pf1N1B4_1041 at 1179.815 kb on + strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.816 kb on - strand, within Pf1N1B4_1041 at 1179.817 kb on + strand, within Pf1N1B4_1041 at 1179.818 kb on - strand, within Pf1N1B4_1041 at 1179.818 kb on - strand, within Pf1N1B4_1041 at 1179.868 kb on + strand, within Pf1N1B4_1041 at 1179.868 kb on + strand, within Pf1N1B4_1041 at 1179.868 kb on + strand, within Pf1N1B4_1041 at 1179.868 kb on + strand, within Pf1N1B4_1041 at 1179.869 kb on - strand, within Pf1N1B4_1041 at 1179.869 kb on - strand, within Pf1N1B4_1041 at 1179.869 kb on - strand, within Pf1N1B4_1041 at 1179.869 kb on - strand, within Pf1N1B4_1041 at 1180.063 kb on + strand at 1180.064 kb on - strand at 1180.064 kb on - strand at 1180.067 kb on - strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.106 kb on + strand at 1180.107 kb on - strand at 1180.107 kb on - strand at 1180.107 kb on - strand at 1180.107 kb on - strand at 1180.107 kb on - strand at 1180.176 kb on - strand, within Pf1N1B4_1042 at 1180.288 kb on + strand, within Pf1N1B4_1042 at 1180.288 kb on + strand, within Pf1N1B4_1042 at 1180.402 kb on + strand, within Pf1N1B4_1042 at 1180.402 kb on + strand, within Pf1N1B4_1042 at 1180.507 kb on + strand at 1180.508 kb on - strand at 1180.508 kb on - strand at 1180.508 kb on - strand at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand at 1180.719 kb on + strand, within Pf1N1B4_1043 at 1180.719 kb on + strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.780 kb on + strand, within Pf1N1B4_1043 at 1180.792 kb on + strand, within Pf1N1B4_1043 at 1180.883 kb on - strand at 1180.928 kb on - strand, within Pf1N1B4_1044 at 1180.953 kb on - strand, within Pf1N1B4_1044 at 1181.279 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.308 kb on + strand at 1181.309 kb on - strand at 1181.309 kb on - strand at 1181.309 kb on - strand at 1181.328 kb on + strand at 1181.328 kb on + strand at 1181.329 kb on - strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.362 kb on - strand at 1181.362 kb on - strand at 1181.366 kb on - strand at 1181.366 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arginine (C) remove 1,179,120 + Pf1N1B4_1040 0.45 +1.0 1,179,120 + Pf1N1B4_1040 0.45 +0.5 1,179,120 + Pf1N1B4_1040 0.45 +1.0 1,179,121 - Pf1N1B4_1040 0.45 +0.7 1,179,121 - Pf1N1B4_1040 0.45 +0.7 1,179,121 - +1.0 1,179,338 + Pf1N1B4_1040 0.76 +0.6 1,179,338 + +2.3 1,179,339 - Pf1N1B4_1040 0.76 +1.3 1,179,427 + Pf1N1B4_1040 0.88 +2.3 1,179,427 + Pf1N1B4_1040 0.88 -1.6 1,179,427 + Pf1N1B4_1040 0.88 -1.6 1,179,427 + Pf1N1B4_1040 0.88 -1.6 1,179,428 - Pf1N1B4_1040 0.88 -0.7 1,179,428 - Pf1N1B4_1040 0.88 -0.6 1,179,428 - Pf1N1B4_1040 0.88 -1.0 1,179,428 - Pf1N1B4_1040 0.88 +0.3 1,179,428 - Pf1N1B4_1040 0.88 +0.7 1,179,442 + +0.2 1,179,460 + -0.4 1,179,460 + +1.0 1,179,460 + -0.6 1,179,461 - -0.3 1,179,461 - +0.3 1,179,461 - -0.6 1,179,508 + -1.6 1,179,508 + -0.3 1,179,508 + -0.6 1,179,508 + +0.0 1,179,508 + +0.0 1,179,541 - +0.7 1,179,573 - -0.4 1,179,659 + Pf1N1B4_1041 0.22 -1.0 1,179,659 + +0.0 1,179,659 + Pf1N1B4_1041 0.22 -2.0 1,179,660 - Pf1N1B4_1041 0.22 +0.5 1,179,660 - +0.0 1,179,660 - Pf1N1B4_1041 0.22 +0.3 1,179,660 - Pf1N1B4_1041 0.22 -0.3 1,179,674 + Pf1N1B4_1041 0.24 -0.3 1,179,675 - Pf1N1B4_1041 0.25 -0.6 1,179,761 + Pf1N1B4_1041 0.40 -0.3 1,179,761 + Pf1N1B4_1041 0.40 +0.0 1,179,762 - Pf1N1B4_1041 0.40 +1.3 1,179,762 - Pf1N1B4_1041 0.40 +1.3 1,179,815 + Pf1N1B4_1041 0.49 -1.0 1,179,815 + Pf1N1B4_1041 0.49 +0.5 1,179,815 + +1.0 1,179,815 + Pf1N1B4_1041 0.49 -0.4 1,179,815 + Pf1N1B4_1041 0.49 +1.0 1,179,816 - Pf1N1B4_1041 0.49 -1.6 1,179,816 - Pf1N1B4_1041 0.49 -0.4 1,179,816 - Pf1N1B4_1041 0.49 +1.6 1,179,816 - Pf1N1B4_1041 0.49 +0.6 1,179,816 - Pf1N1B4_1041 0.49 +1.6 1,179,816 - Pf1N1B4_1041 0.49 +0.5 1,179,816 - Pf1N1B4_1041 0.49 -2.3 1,179,816 - Pf1N1B4_1041 0.49 +1.0 1,179,816 - Pf1N1B4_1041 0.49 +1.0 1,179,816 - Pf1N1B4_1041 0.49 -1.6 1,179,816 - Pf1N1B4_1041 0.49 +0.0 1,179,816 - Pf1N1B4_1041 0.49 -0.6 1,179,816 - Pf1N1B4_1041 0.49 -0.4 1,179,817 + Pf1N1B4_1041 0.49 +0.0 1,179,818 - Pf1N1B4_1041 0.49 -0.8 1,179,818 - Pf1N1B4_1041 0.49 +0.0 1,179,868 + Pf1N1B4_1041 0.58 +0.3 1,179,868 + Pf1N1B4_1041 0.58 +1.3 1,179,868 + Pf1N1B4_1041 0.58 -0.6 1,179,868 + Pf1N1B4_1041 0.58 -2.8 1,179,869 - Pf1N1B4_1041 0.58 -0.3 1,179,869 - Pf1N1B4_1041 0.58 +0.5 1,179,869 - Pf1N1B4_1041 0.58 +2.0 1,179,869 - Pf1N1B4_1041 0.58 +0.0 1,180,063 + -1.0 1,180,064 - +1.6 1,180,064 - +2.0 1,180,067 - -0.7 1,180,106 + +1.0 1,180,106 + -1.0 1,180,106 + +0.4 1,180,106 + +1.0 1,180,106 + +0.0 1,180,106 + +0.0 1,180,106 + +2.0 1,180,107 - -0.4 1,180,107 - +0.0 1,180,107 - +0.2 1,180,107 - +0.3 1,180,107 - -1.6 1,180,176 - Pf1N1B4_1042 0.17 +2.0 1,180,288 + Pf1N1B4_1042 0.48 +0.3 1,180,288 + Pf1N1B4_1042 0.48 +0.4 1,180,402 + Pf1N1B4_1042 0.78 +2.0 1,180,402 + Pf1N1B4_1042 0.78 +0.5 1,180,507 + +0.0 1,180,508 - +0.0 1,180,508 - -0.1 1,180,508 - +0.2 1,180,717 + Pf1N1B4_1043 0.46 -0.7 1,180,717 + Pf1N1B4_1043 0.46 -0.4 1,180,717 + Pf1N1B4_1043 0.46 +0.0 1,180,717 + Pf1N1B4_1043 0.46 -0.5 1,180,717 + Pf1N1B4_1043 0.46 +0.0 1,180,717 + Pf1N1B4_1043 0.46 -0.6 1,180,717 + Pf1N1B4_1043 0.46 +0.0 1,180,717 + Pf1N1B4_1043 0.46 -0.3 1,180,717 + Pf1N1B4_1043 0.46 -2.2 1,180,717 + Pf1N1B4_1043 0.46 +1.6 1,180,717 + Pf1N1B4_1043 0.46 -0.6 1,180,717 + Pf1N1B4_1043 0.46 +0.3 1,180,718 - Pf1N1B4_1043 0.47 -1.0 1,180,718 - Pf1N1B4_1043 0.47 +0.9 1,180,718 - Pf1N1B4_1043 0.47 +0.0 1,180,718 - +0.0 1,180,718 - Pf1N1B4_1043 0.47 +1.3 1,180,718 - +0.4 1,180,719 + Pf1N1B4_1043 0.47 -0.6 1,180,719 + Pf1N1B4_1043 0.47 +0.3 1,180,720 - Pf1N1B4_1043 0.47 -0.6 1,180,720 - Pf1N1B4_1043 0.47 -1.6 1,180,720 - Pf1N1B4_1043 0.47 -2.6 1,180,720 - Pf1N1B4_1043 0.47 +0.0 1,180,780 + Pf1N1B4_1043 0.68 +1.3 1,180,792 + Pf1N1B4_1043 0.72 +0.4 1,180,883 - -0.4 1,180,928 - Pf1N1B4_1044 0.18 +0.0 1,180,953 - Pf1N1B4_1044 0.25 -1.6 1,181,279 + +3.2 1,181,285 + +0.1 1,181,285 + -1.0 1,181,285 + +0.0 1,181,285 + -0.3 1,181,286 - -1.6 1,181,286 - +0.0 1,181,286 - +0.9 1,181,286 - +0.0 1,181,286 - +0.3 1,181,308 + +0.6 1,181,309 - -0.4 1,181,309 - -1.0 1,181,309 - -1.6 1,181,328 + +0.2 1,181,328 + +0.2 1,181,329 - +1.2 1,181,361 + +0.6 1,181,361 + +0.3 1,181,361 + +2.0 1,181,361 + +0.0 1,181,362 - +0.0 1,181,362 - -0.2 1,181,366 - -0.8 1,181,366 - +2.6
Or see this region's nucleotide sequence