Experiment: Recovery broth with Aztreonam 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14375 and BBR_RS14380 are separated by 131 nucleotides BBR_RS14380 and BBR_RS14390 are separated by 244 nucleotides BBR_RS14390 and BBR_RS14395 are separated by 102 nucleotides
BBR_RS14375: BBR_RS14375 - acetate kinase, at 999,106 to 1,000,335
_RS14375
BBR_RS14380: BBR_RS14380 - phosphate acetyltransferase, at 1,000,467 to 1,002,137
_RS14380
BBR_RS14390: BBR_RS14390 - tRNA-Gln, at 1,002,382 to 1,002,455
_RS14390
BBR_RS14395: BBR_RS14395 - ribose-phosphate diphosphokinase, at 1,002,558 to 1,003,580
_RS14395
Position (kb)
1000
1001
1002
1003 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1000.432 kb on - strand at 1000.442 kb on + strand at 1000.498 kb on - strand at 1000.517 kb on - strand at 1000.517 kb on - strand at 1000.533 kb on - strand at 1000.533 kb on - strand at 1000.594 kb on - strand at 1000.606 kb on + strand at 1000.614 kb on - strand at 1000.615 kb on - strand at 1000.616 kb on - strand at 1000.686 kb on - strand, within BBR_RS14380 at 1000.697 kb on - strand, within BBR_RS14380 at 1000.749 kb on + strand, within BBR_RS14380 at 1000.756 kb on - strand, within BBR_RS14380 at 1000.757 kb on - strand, within BBR_RS14380 at 1000.772 kb on + strand, within BBR_RS14380 at 1000.772 kb on + strand, within BBR_RS14380 at 1000.794 kb on + strand, within BBR_RS14380 at 1000.808 kb on - strand, within BBR_RS14380 at 1000.828 kb on + strand, within BBR_RS14380 at 1000.836 kb on - strand, within BBR_RS14380 at 1000.836 kb on - strand, within BBR_RS14380 at 1000.836 kb on - strand, within BBR_RS14380 at 1000.836 kb on - strand, within BBR_RS14380 at 1000.836 kb on - strand, within BBR_RS14380 at 1000.836 kb on - strand, within BBR_RS14380 at 1000.838 kb on - strand, within BBR_RS14380 at 1000.921 kb on + strand, within BBR_RS14380 at 1000.947 kb on - strand, within BBR_RS14380 at 1000.980 kb on - strand, within BBR_RS14380 at 1000.998 kb on + strand, within BBR_RS14380 at 1001.006 kb on - strand, within BBR_RS14380 at 1001.006 kb on - strand, within BBR_RS14380 at 1001.008 kb on + strand, within BBR_RS14380 at 1001.008 kb on + strand, within BBR_RS14380 at 1001.022 kb on - strand, within BBR_RS14380 at 1001.032 kb on - strand, within BBR_RS14380 at 1001.073 kb on + strand, within BBR_RS14380 at 1001.076 kb on - strand, within BBR_RS14380 at 1001.099 kb on - strand, within BBR_RS14380 at 1001.145 kb on + strand, within BBR_RS14380 at 1001.165 kb on + strand, within BBR_RS14380 at 1001.166 kb on - strand, within BBR_RS14380 at 1001.205 kb on + strand, within BBR_RS14380 at 1001.205 kb on + strand, within BBR_RS14380 at 1001.205 kb on - strand, within BBR_RS14380 at 1001.247 kb on + strand, within BBR_RS14380 at 1001.247 kb on + strand, within BBR_RS14380 at 1001.303 kb on - strand, within BBR_RS14380 at 1001.374 kb on + strand, within BBR_RS14380 at 1001.374 kb on + strand, within BBR_RS14380 at 1001.382 kb on - strand, within BBR_RS14380 at 1001.382 kb on - strand, within BBR_RS14380 at 1001.382 kb on - strand, within BBR_RS14380 at 1001.382 kb on - strand, within BBR_RS14380 at 1001.384 kb on + strand, within BBR_RS14380 at 1001.412 kb on - strand, within BBR_RS14380 at 1001.451 kb on - strand, within BBR_RS14380 at 1001.453 kb on + strand, within BBR_RS14380 at 1001.460 kb on + strand, within BBR_RS14380 at 1001.460 kb on - strand, within BBR_RS14380 at 1001.494 kb on - strand, within BBR_RS14380 at 1001.494 kb on - strand, within BBR_RS14380 at 1001.508 kb on - strand, within BBR_RS14380 at 1001.522 kb on - strand, within BBR_RS14380 at 1001.525 kb on - strand, within BBR_RS14380 at 1001.525 kb on - strand, within BBR_RS14380 at 1001.558 kb on - strand, within BBR_RS14380 at 1001.679 kb on - strand, within BBR_RS14380 at 1001.679 kb on - strand, within BBR_RS14380 at 1001.701 kb on + strand, within BBR_RS14380 at 1001.701 kb on + strand, within BBR_RS14380 at 1001.709 kb on - strand, within BBR_RS14380 at 1001.713 kb on - strand, within BBR_RS14380 at 1001.851 kb on + strand, within BBR_RS14380 at 1001.856 kb on + strand, within BBR_RS14380 at 1001.856 kb on + strand, within BBR_RS14380 at 1001.859 kb on - strand, within BBR_RS14380 at 1001.861 kb on + strand, within BBR_RS14380 at 1001.864 kb on - strand, within BBR_RS14380 at 1001.864 kb on - strand, within BBR_RS14380 at 1001.864 kb on - strand, within BBR_RS14380 at 1001.864 kb on - strand, within BBR_RS14380 at 1001.864 kb on - strand, within BBR_RS14380 at 1001.864 kb on - strand, within BBR_RS14380 at 1001.869 kb on - strand, within BBR_RS14380 at 1001.907 kb on + strand, within BBR_RS14380 at 1001.917 kb on - strand, within BBR_RS14380 at 1001.941 kb on + strand, within BBR_RS14380 at 1001.946 kb on + strand, within BBR_RS14380 at 1001.959 kb on - strand, within BBR_RS14380 at 1001.987 kb on + strand at 1002.030 kb on - strand at 1002.037 kb on + strand at 1002.038 kb on - strand at 1002.045 kb on - strand at 1002.101 kb on + strand at 1002.119 kb on + strand at 1002.125 kb on - strand at 1002.126 kb on + strand at 1002.131 kb on + strand at 1002.137 kb on + strand at 1002.162 kb on + strand at 1002.181 kb on + strand at 1002.232 kb on + strand at 1002.232 kb on + strand at 1002.521 kb on - strand at 1002.521 kb on - strand at 1002.549 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Aztreonam 0.2 mM remove 1,000,432 - +0.1 1,000,442 + +1.0 1,000,498 - +1.3 1,000,517 - +0.4 1,000,517 - +0.6 1,000,533 - +1.5 1,000,533 - +1.9 1,000,594 - -0.1 1,000,606 + +1.2 1,000,614 - +0.6 1,000,615 - -0.5 1,000,616 - +0.5 1,000,686 - BBR_RS14380 0.13 +0.3 1,000,697 - BBR_RS14380 0.14 +0.9 1,000,749 + BBR_RS14380 0.17 +0.8 1,000,756 - BBR_RS14380 0.17 -0.9 1,000,757 - BBR_RS14380 0.17 +0.7 1,000,772 + BBR_RS14380 0.18 +0.1 1,000,772 + BBR_RS14380 0.18 +0.9 1,000,794 + BBR_RS14380 0.20 +1.0 1,000,808 - BBR_RS14380 0.20 +0.2 1,000,828 + BBR_RS14380 0.22 -0.1 1,000,836 - BBR_RS14380 0.22 +0.3 1,000,836 - BBR_RS14380 0.22 +1.0 1,000,836 - BBR_RS14380 0.22 +1.1 1,000,836 - BBR_RS14380 0.22 +0.9 1,000,836 - BBR_RS14380 0.22 +1.5 1,000,836 - BBR_RS14380 0.22 +2.1 1,000,838 - BBR_RS14380 0.22 +2.2 1,000,921 + BBR_RS14380 0.27 -0.2 1,000,947 - BBR_RS14380 0.29 +0.4 1,000,980 - BBR_RS14380 0.31 +0.1 1,000,998 + BBR_RS14380 0.32 +2.1 1,001,006 - BBR_RS14380 0.32 +0.5 1,001,006 - BBR_RS14380 0.32 +0.6 1,001,008 + BBR_RS14380 0.32 +2.2 1,001,008 + BBR_RS14380 0.32 +0.6 1,001,022 - BBR_RS14380 0.33 -0.5 1,001,032 - BBR_RS14380 0.34 +1.2 1,001,073 + BBR_RS14380 0.36 +0.6 1,001,076 - BBR_RS14380 0.36 -0.1 1,001,099 - BBR_RS14380 0.38 +1.5 1,001,145 + BBR_RS14380 0.41 -0.7 1,001,165 + BBR_RS14380 0.42 +0.5 1,001,166 - BBR_RS14380 0.42 +0.4 1,001,205 + BBR_RS14380 0.44 -0.5 1,001,205 + BBR_RS14380 0.44 +0.1 1,001,205 - BBR_RS14380 0.44 +0.1 1,001,247 + BBR_RS14380 0.47 +1.0 1,001,247 + BBR_RS14380 0.47 +0.5 1,001,303 - BBR_RS14380 0.50 +0.2 1,001,374 + BBR_RS14380 0.54 +0.6 1,001,374 + BBR_RS14380 0.54 +0.9 1,001,382 - BBR_RS14380 0.55 -0.4 1,001,382 - BBR_RS14380 0.55 -2.3 1,001,382 - BBR_RS14380 0.55 +1.5 1,001,382 - BBR_RS14380 0.55 +1.2 1,001,384 + BBR_RS14380 0.55 +1.3 1,001,412 - BBR_RS14380 0.57 -0.2 1,001,451 - BBR_RS14380 0.59 -0.8 1,001,453 + BBR_RS14380 0.59 +1.5 1,001,460 + BBR_RS14380 0.59 +0.7 1,001,460 - BBR_RS14380 0.59 +0.1 1,001,494 - BBR_RS14380 0.61 +1.2 1,001,494 - BBR_RS14380 0.61 +0.4 1,001,508 - BBR_RS14380 0.62 -0.5 1,001,522 - BBR_RS14380 0.63 +1.3 1,001,525 - BBR_RS14380 0.63 +2.2 1,001,525 - BBR_RS14380 0.63 +2.3 1,001,558 - BBR_RS14380 0.65 -0.2 1,001,679 - BBR_RS14380 0.73 -0.2 1,001,679 - BBR_RS14380 0.73 +0.4 1,001,701 + BBR_RS14380 0.74 +2.2 1,001,701 + BBR_RS14380 0.74 -1.1 1,001,709 - BBR_RS14380 0.74 +1.3 1,001,713 - BBR_RS14380 0.75 +1.4 1,001,851 + BBR_RS14380 0.83 +0.8 1,001,856 + BBR_RS14380 0.83 +1.2 1,001,856 + BBR_RS14380 0.83 +0.9 1,001,859 - BBR_RS14380 0.83 +0.4 1,001,861 + BBR_RS14380 0.83 -0.3 1,001,864 - BBR_RS14380 0.84 +1.1 1,001,864 - BBR_RS14380 0.84 +1.0 1,001,864 - BBR_RS14380 0.84 +1.4 1,001,864 - BBR_RS14380 0.84 +0.2 1,001,864 - BBR_RS14380 0.84 +0.4 1,001,864 - BBR_RS14380 0.84 +0.6 1,001,869 - BBR_RS14380 0.84 +0.0 1,001,907 + BBR_RS14380 0.86 +1.4 1,001,917 - BBR_RS14380 0.87 -1.3 1,001,941 + BBR_RS14380 0.88 +4.5 1,001,946 + BBR_RS14380 0.89 +0.9 1,001,959 - BBR_RS14380 0.89 -0.5 1,001,987 + +1.1 1,002,030 - +0.7 1,002,037 + -0.1 1,002,038 - +1.0 1,002,045 - +0.2 1,002,101 + -0.3 1,002,119 + -1.7 1,002,125 - +0.1 1,002,126 + +2.0 1,002,131 + +1.1 1,002,137 + +1.3 1,002,162 + +0.1 1,002,181 + -0.4 1,002,232 + -0.2 1,002,232 + -0.5 1,002,521 - +0.9 1,002,521 - +0.2 1,002,549 + +1.6
Or see this region's nucleotide sequence