Experiment: Choline chloride 358 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_10995 and AO353_11000 are separated by 135 nucleotides AO353_11000 and AO353_11005 overlap by 4 nucleotides AO353_11005 and AO353_11010 are separated by 1 nucleotides AO353_11010 and AO353_11015 overlap by 4 nucleotides
AO353_10995: AO353_10995 - hypothetical protein, at 2,402,706 to 2,403,935
_10995
AO353_11000: AO353_11000 - ABC transporter, at 2,404,071 to 2,404,712
_11000
AO353_11005: AO353_11005 - ABC transporter permease, at 2,404,709 to 2,405,647
_11005
AO353_11010: AO353_11010 - ABC transporter ATP-binding protein, at 2,405,649 to 2,406,452
_11010
AO353_11015: AO353_11015 - ABC transporter permease, at 2,406,449 to 2,407,594
_11015
Position (kb)
2404
2405
2406 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2403.779 kb on + strand, within AO353_10995 at 2403.780 kb on - strand, within AO353_10995 at 2403.780 kb on - strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.807 kb on + strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.808 kb on - strand, within AO353_10995 at 2403.822 kb on + strand at 2403.823 kb on - strand at 2403.823 kb on - strand at 2403.823 kb on - strand at 2403.942 kb on + strand at 2403.943 kb on - strand at 2403.987 kb on + strand at 2403.987 kb on + strand at 2403.987 kb on + strand at 2403.987 kb on + strand at 2403.987 kb on + strand at 2404.009 kb on - strand at 2404.009 kb on - strand at 2404.033 kb on - strand at 2404.080 kb on + strand at 2404.080 kb on + strand at 2404.080 kb on + strand at 2404.081 kb on - strand at 2404.081 kb on - strand at 2404.081 kb on - strand at 2404.081 kb on - strand at 2404.091 kb on + strand at 2404.092 kb on - strand at 2404.092 kb on - strand at 2404.092 kb on - strand at 2404.092 kb on - strand at 2404.110 kb on + strand at 2404.300 kb on + strand, within AO353_11000 at 2404.301 kb on - strand, within AO353_11000 at 2404.301 kb on - strand, within AO353_11000 at 2404.301 kb on - strand, within AO353_11000 at 2404.380 kb on + strand, within AO353_11000 at 2404.380 kb on + strand, within AO353_11000 at 2404.380 kb on + strand, within AO353_11000 at 2404.380 kb on + strand, within AO353_11000 at 2404.380 kb on + strand, within AO353_11000 at 2404.381 kb on - strand, within AO353_11000 at 2404.381 kb on - strand, within AO353_11000 at 2404.437 kb on - strand, within AO353_11000 at 2404.437 kb on - strand, within AO353_11000 at 2404.462 kb on + strand, within AO353_11000 at 2404.462 kb on + strand, within AO353_11000 at 2404.462 kb on + strand, within AO353_11000 at 2404.462 kb on + strand, within AO353_11000 at 2404.463 kb on - strand, within AO353_11000 at 2404.463 kb on - strand, within AO353_11000 at 2404.463 kb on - strand, within AO353_11000 at 2404.463 kb on - strand, within AO353_11000 at 2404.463 kb on - strand, within AO353_11000 at 2404.463 kb on - strand, within AO353_11000 at 2404.535 kb on + strand, within AO353_11000 at 2404.535 kb on + strand, within AO353_11000 at 2404.535 kb on + strand, within AO353_11000 at 2404.535 kb on + strand, within AO353_11000 at 2404.536 kb on - strand, within AO353_11000 at 2404.536 kb on - strand, within AO353_11000 at 2404.536 kb on - strand, within AO353_11000 at 2404.536 kb on - strand, within AO353_11000 at 2404.536 kb on - strand, within AO353_11000 at 2404.545 kb on + strand, within AO353_11000 at 2404.545 kb on + strand, within AO353_11000 at 2404.546 kb on - strand, within AO353_11000 at 2404.636 kb on - strand, within AO353_11000 at 2404.636 kb on - strand, within AO353_11000 at 2404.750 kb on - strand at 2404.750 kb on - strand at 2404.790 kb on + strand at 2404.791 kb on - strand at 2404.791 kb on - strand at 2404.820 kb on + strand, within AO353_11005 at 2404.820 kb on + strand, within AO353_11005 at 2404.820 kb on + strand, within AO353_11005 at 2404.820 kb on + strand, within AO353_11005 at 2404.820 kb on + strand, within AO353_11005 at 2404.820 kb on + strand, within AO353_11005 at 2404.820 kb on + strand, within AO353_11005 at 2404.820 kb on + strand, within AO353_11005 at 2404.975 kb on + strand, within AO353_11005 at 2404.975 kb on + strand, within AO353_11005 at 2404.976 kb on - strand, within AO353_11005 at 2404.976 kb on - strand, within AO353_11005 at 2404.976 kb on - strand, within AO353_11005 at 2405.263 kb on + strand, within AO353_11005 at 2405.264 kb on - strand, within AO353_11005 at 2405.264 kb on - strand, within AO353_11005 at 2405.264 kb on - strand, within AO353_11005 at 2405.264 kb on - strand, within AO353_11005 at 2405.280 kb on - strand, within AO353_11005 at 2405.447 kb on + strand, within AO353_11005 at 2405.447 kb on + strand, within AO353_11005 at 2405.447 kb on + strand, within AO353_11005 at 2405.447 kb on + strand, within AO353_11005 at 2405.448 kb on - strand, within AO353_11005 at 2405.448 kb on - strand, within AO353_11005 at 2405.448 kb on - strand, within AO353_11005 at 2405.448 kb on - strand, within AO353_11005 at 2405.448 kb on - strand, within AO353_11005 at 2405.448 kb on - strand, within AO353_11005 at 2405.448 kb on - strand, within AO353_11005 at 2405.472 kb on + strand, within AO353_11005 at 2405.472 kb on + strand, within AO353_11005 at 2405.472 kb on + strand, within AO353_11005 at 2405.472 kb on + strand, within AO353_11005 at 2405.472 kb on + strand, within AO353_11005 at 2405.473 kb on - strand, within AO353_11005 at 2405.529 kb on + strand, within AO353_11005 at 2405.529 kb on + strand, within AO353_11005 at 2405.529 kb on + strand, within AO353_11005 at 2405.529 kb on + strand, within AO353_11005 at 2405.530 kb on - strand, within AO353_11005 at 2405.530 kb on - strand, within AO353_11005 at 2405.604 kb on - strand at 2405.604 kb on - strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.624 kb on + strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.625 kb on - strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.652 kb on + strand at 2405.653 kb on - strand at 2405.653 kb on - strand at 2405.653 kb on - strand at 2405.653 kb on - strand at 2405.710 kb on + strand at 2405.710 kb on + strand at 2405.710 kb on + strand at 2405.710 kb on + strand at 2405.711 kb on - strand at 2405.711 kb on - strand at 2405.711 kb on - strand at 2405.711 kb on - strand at 2405.711 kb on - strand at 2405.711 kb on - strand at 2405.769 kb on + strand, within AO353_11010 at 2405.769 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.812 kb on + strand, within AO353_11010 at 2405.813 kb on - strand, within AO353_11010 at 2405.813 kb on - strand, within AO353_11010 at 2405.813 kb on - strand, within AO353_11010 at 2405.813 kb on - strand, within AO353_11010 at 2405.813 kb on - strand at 2405.813 kb on - strand, within AO353_11010 at 2405.819 kb on + strand, within AO353_11010 at 2405.820 kb on - strand, within AO353_11010 at 2406.017 kb on - strand, within AO353_11010 at 2406.346 kb on + strand, within AO353_11010 at 2406.346 kb on + strand, within AO353_11010 at 2406.346 kb on + strand, within AO353_11010 at 2406.346 kb on + strand, within AO353_11010 at 2406.346 kb on + strand, within AO353_11010 at 2406.346 kb on + strand, within AO353_11010 at 2406.346 kb on + strand, within AO353_11010 at 2406.346 kb on + strand, within AO353_11010 at 2406.347 kb on - strand, within AO353_11010 at 2406.347 kb on - strand, within AO353_11010 at 2406.347 kb on - strand, within AO353_11010 at 2406.347 kb on - strand, within AO353_11010 at 2406.347 kb on - strand, within AO353_11010 at 2406.347 kb on - strand, within AO353_11010 at 2406.347 kb on - strand, within AO353_11010 at 2406.347 kb on - strand, within AO353_11010 at 2406.434 kb on + strand at 2406.435 kb on - strand at 2406.466 kb on + strand at 2406.467 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 358 mM remove 2,403,779 + AO353_10995 0.87 +0.5 2,403,780 - AO353_10995 0.87 -1.9 2,403,780 - AO353_10995 0.87 -0.7 2,403,807 + AO353_10995 0.90 +0.5 2,403,807 + AO353_10995 0.90 -0.9 2,403,807 + AO353_10995 0.90 -0.3 2,403,807 + AO353_10995 0.90 +0.5 2,403,807 + AO353_10995 0.90 +0.8 2,403,807 + AO353_10995 0.90 -0.3 2,403,807 + AO353_10995 0.90 -0.5 2,403,807 + AO353_10995 0.90 -1.8 2,403,807 + AO353_10995 0.90 +0.4 2,403,807 + AO353_10995 0.90 -0.9 2,403,807 + AO353_10995 0.90 -0.1 2,403,807 + AO353_10995 0.90 +0.7 2,403,807 + AO353_10995 0.90 -1.9 2,403,807 + AO353_10995 0.90 -0.2 2,403,807 + AO353_10995 0.90 -0.7 2,403,807 + AO353_10995 0.90 +0.6 2,403,807 + AO353_10995 0.90 +1.3 2,403,807 + AO353_10995 0.90 -0.4 2,403,807 + AO353_10995 0.90 +1.3 2,403,807 + AO353_10995 0.90 +0.8 2,403,807 + AO353_10995 0.90 -0.0 2,403,807 + AO353_10995 0.90 -0.3 2,403,807 + AO353_10995 0.90 -1.5 2,403,808 - AO353_10995 0.90 +1.3 2,403,808 - AO353_10995 0.90 -0.3 2,403,808 - AO353_10995 0.90 -0.7 2,403,808 - AO353_10995 0.90 +0.3 2,403,808 - AO353_10995 0.90 +1.8 2,403,808 - AO353_10995 0.90 +0.7 2,403,808 - AO353_10995 0.90 +0.0 2,403,808 - AO353_10995 0.90 +0.2 2,403,808 - AO353_10995 0.90 -1.5 2,403,808 - AO353_10995 0.90 +1.6 2,403,808 - AO353_10995 0.90 -2.2 2,403,808 - AO353_10995 0.90 +0.3 2,403,808 - AO353_10995 0.90 +0.1 2,403,808 - AO353_10995 0.90 +0.1 2,403,808 - AO353_10995 0.90 +0.7 2,403,808 - AO353_10995 0.90 +0.1 2,403,808 - AO353_10995 0.90 -0.0 2,403,808 - AO353_10995 0.90 +0.2 2,403,808 - AO353_10995 0.90 -0.7 2,403,822 + +0.4 2,403,823 - -0.0 2,403,823 - -1.3 2,403,823 - -0.6 2,403,942 + -1.0 2,403,943 - +0.8 2,403,987 + +1.5 2,403,987 + +1.0 2,403,987 + +0.5 2,403,987 + +1.6 2,403,987 + +0.3 2,404,009 - +0.5 2,404,009 - -0.5 2,404,033 - -0.6 2,404,080 + +0.4 2,404,080 + +1.7 2,404,080 + +0.5 2,404,081 - +0.5 2,404,081 - -2.2 2,404,081 - -0.3 2,404,081 - -0.1 2,404,091 + -0.2 2,404,092 - +0.7 2,404,092 - -1.5 2,404,092 - -1.4 2,404,092 - -0.3 2,404,110 + -0.0 2,404,300 + AO353_11000 0.36 -0.9 2,404,301 - AO353_11000 0.36 +0.3 2,404,301 - AO353_11000 0.36 +1.8 2,404,301 - AO353_11000 0.36 -0.3 2,404,380 + AO353_11000 0.48 -0.2 2,404,380 + AO353_11000 0.48 -1.3 2,404,380 + AO353_11000 0.48 +0.7 2,404,380 + AO353_11000 0.48 -0.5 2,404,380 + AO353_11000 0.48 +0.8 2,404,381 - AO353_11000 0.48 -0.7 2,404,381 - AO353_11000 0.48 -0.3 2,404,437 - AO353_11000 0.57 -1.4 2,404,437 - AO353_11000 0.57 -1.2 2,404,462 + AO353_11000 0.61 -1.5 2,404,462 + AO353_11000 0.61 +1.0 2,404,462 + AO353_11000 0.61 -0.2 2,404,462 + AO353_11000 0.61 +1.0 2,404,463 - AO353_11000 0.61 +0.4 2,404,463 - AO353_11000 0.61 -1.5 2,404,463 - AO353_11000 0.61 +0.3 2,404,463 - AO353_11000 0.61 -0.7 2,404,463 - AO353_11000 0.61 -0.2 2,404,463 - AO353_11000 0.61 -0.1 2,404,535 + AO353_11000 0.72 +0.4 2,404,535 + AO353_11000 0.72 +1.4 2,404,535 + AO353_11000 0.72 -0.0 2,404,535 + AO353_11000 0.72 -0.3 2,404,536 - AO353_11000 0.72 +2.3 2,404,536 - AO353_11000 0.72 +0.3 2,404,536 - AO353_11000 0.72 +1.1 2,404,536 - AO353_11000 0.72 +0.3 2,404,536 - AO353_11000 0.72 -1.9 2,404,545 + AO353_11000 0.74 -2.8 2,404,545 + AO353_11000 0.74 -1.0 2,404,546 - AO353_11000 0.74 +1.7 2,404,636 - AO353_11000 0.88 +0.6 2,404,636 - AO353_11000 0.88 -0.1 2,404,750 - +0.4 2,404,750 - -0.1 2,404,790 + -2.4 2,404,791 - -0.9 2,404,791 - +1.0 2,404,820 + AO353_11005 0.12 -0.9 2,404,820 + AO353_11005 0.12 -0.9 2,404,820 + AO353_11005 0.12 -1.2 2,404,820 + AO353_11005 0.12 +0.1 2,404,820 + AO353_11005 0.12 +1.7 2,404,820 + AO353_11005 0.12 -1.0 2,404,820 + AO353_11005 0.12 +0.3 2,404,820 + AO353_11005 0.12 -0.1 2,404,975 + AO353_11005 0.28 -1.5 2,404,975 + AO353_11005 0.28 -0.7 2,404,976 - AO353_11005 0.28 -0.7 2,404,976 - AO353_11005 0.28 -0.0 2,404,976 - AO353_11005 0.28 -0.2 2,405,263 + AO353_11005 0.59 -0.7 2,405,264 - AO353_11005 0.59 +0.1 2,405,264 - AO353_11005 0.59 -2.7 2,405,264 - AO353_11005 0.59 -0.3 2,405,264 - AO353_11005 0.59 +0.6 2,405,280 - AO353_11005 0.61 -0.3 2,405,447 + AO353_11005 0.79 +0.7 2,405,447 + AO353_11005 0.79 -1.2 2,405,447 + AO353_11005 0.79 +2.3 2,405,447 + AO353_11005 0.79 -2.1 2,405,448 - AO353_11005 0.79 -1.5 2,405,448 - AO353_11005 0.79 -0.5 2,405,448 - AO353_11005 0.79 -0.9 2,405,448 - AO353_11005 0.79 -0.4 2,405,448 - AO353_11005 0.79 -0.8 2,405,448 - AO353_11005 0.79 -0.7 2,405,448 - AO353_11005 0.79 -1.5 2,405,472 + AO353_11005 0.81 +0.4 2,405,472 + AO353_11005 0.81 +0.6 2,405,472 + AO353_11005 0.81 -1.3 2,405,472 + AO353_11005 0.81 +2.5 2,405,472 + AO353_11005 0.81 +0.1 2,405,473 - AO353_11005 0.81 +0.3 2,405,529 + AO353_11005 0.87 +0.4 2,405,529 + AO353_11005 0.87 +1.0 2,405,529 + AO353_11005 0.87 -0.3 2,405,529 + AO353_11005 0.87 -1.3 2,405,530 - AO353_11005 0.87 -1.6 2,405,530 - AO353_11005 0.87 -0.3 2,405,604 - -0.0 2,405,604 - +0.7 2,405,624 + -1.8 2,405,624 + +0.2 2,405,624 + +1.0 2,405,624 + -0.3 2,405,624 + -2.0 2,405,624 + +0.1 2,405,624 + +1.3 2,405,624 + -1.5 2,405,624 + +0.8 2,405,624 + -0.0 2,405,624 + -0.6 2,405,624 + -0.5 2,405,624 + +0.5 2,405,625 - -0.3 2,405,625 - +1.0 2,405,625 - -0.1 2,405,625 - -0.3 2,405,625 - +1.1 2,405,625 - -0.5 2,405,625 - -0.1 2,405,625 - +0.1 2,405,625 - -0.5 2,405,625 - +0.5 2,405,625 - -1.0 2,405,625 - +0.3 2,405,625 - -1.5 2,405,625 - +0.7 2,405,625 - +0.3 2,405,625 - +0.3 2,405,625 - -0.4 2,405,625 - -0.5 2,405,625 - -0.2 2,405,652 + +1.3 2,405,652 + -0.9 2,405,652 + -2.0 2,405,652 + -0.1 2,405,652 + -0.3 2,405,652 + +0.6 2,405,652 + -0.3 2,405,652 + +0.6 2,405,652 + +0.0 2,405,652 + -0.7 2,405,652 + +0.5 2,405,653 - +0.1 2,405,653 - -2.2 2,405,653 - +0.5 2,405,653 - -0.3 2,405,710 + +0.4 2,405,710 + -0.5 2,405,710 + +0.6 2,405,710 + -0.3 2,405,711 - -0.7 2,405,711 - -0.0 2,405,711 - +0.7 2,405,711 - +0.5 2,405,711 - -0.2 2,405,711 - +0.5 2,405,769 + AO353_11010 0.15 -0.2 2,405,769 + AO353_11010 0.15 -0.7 2,405,812 + AO353_11010 0.20 -0.0 2,405,812 + AO353_11010 0.20 +1.0 2,405,812 + AO353_11010 0.20 +1.1 2,405,812 + AO353_11010 0.20 +0.9 2,405,812 + AO353_11010 0.20 +0.1 2,405,812 + AO353_11010 0.20 -0.4 2,405,812 + AO353_11010 0.20 +1.2 2,405,812 + AO353_11010 0.20 -0.2 2,405,812 + AO353_11010 0.20 -0.5 2,405,812 + AO353_11010 0.20 -0.1 2,405,812 + AO353_11010 0.20 +1.0 2,405,813 - AO353_11010 0.20 +0.4 2,405,813 - AO353_11010 0.20 +2.9 2,405,813 - AO353_11010 0.20 +0.5 2,405,813 - AO353_11010 0.20 +0.0 2,405,813 - +1.3 2,405,813 - AO353_11010 0.20 -0.1 2,405,819 + AO353_11010 0.21 +0.6 2,405,820 - AO353_11010 0.21 -0.6 2,406,017 - AO353_11010 0.46 -1.0 2,406,346 + AO353_11010 0.87 -0.3 2,406,346 + AO353_11010 0.87 -1.7 2,406,346 + AO353_11010 0.87 -0.5 2,406,346 + AO353_11010 0.87 +0.4 2,406,346 + AO353_11010 0.87 +0.0 2,406,346 + AO353_11010 0.87 -1.7 2,406,346 + AO353_11010 0.87 -1.9 2,406,346 + AO353_11010 0.87 -0.5 2,406,347 - AO353_11010 0.87 -1.3 2,406,347 - AO353_11010 0.87 +0.7 2,406,347 - AO353_11010 0.87 -1.0 2,406,347 - AO353_11010 0.87 +0.3 2,406,347 - AO353_11010 0.87 +0.1 2,406,347 - AO353_11010 0.87 +0.3 2,406,347 - AO353_11010 0.87 +0.4 2,406,347 - AO353_11010 0.87 -0.1 2,406,434 + +0.3 2,406,435 - -0.9 2,406,466 + -0.8 2,406,467 - -0.2
Or see this region's nucleotide sequence