Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_04500

Experiment: Choline chloride 358 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_04490 and AO353_04495 are separated by 124 nucleotidesAO353_04495 and AO353_04500 are separated by 49 nucleotidesAO353_04500 and AO353_04505 are separated by 201 nucleotides AO353_04490: AO353_04490 - murein transglycosylase, at 968,939 to 970,399 _04490 AO353_04495: AO353_04495 - pyridoxine 5'-phosphate synthase, at 970,524 to 971,267 _04495 AO353_04500: AO353_04500 - DNA repair protein RecO, at 971,317 to 972,006 _04500 AO353_04505: AO353_04505 - GTPase Era, at 972,208 to 973,110 _04505 Position (kb) 971 972 973Strain fitness (log2 ratio) -2 -1 0 1 2at 970.397 kb on + strandat 970.398 kb on - strandat 971.307 kb on + strandat 971.307 kb on + strandat 971.308 kb on - strandat 971.486 kb on + strand, within AO353_04500at 971.486 kb on + strand, within AO353_04500at 971.541 kb on + strand, within AO353_04500at 971.669 kb on + strand, within AO353_04500at 971.735 kb on + strand, within AO353_04500at 971.736 kb on - strand, within AO353_04500at 971.736 kb on - strand, within AO353_04500at 971.941 kb on - strandat 971.941 kb on - strandat 971.961 kb on + strandat 971.972 kb on + strandat 971.973 kb on - strandat 971.973 kb on - strandat 971.973 kb on - strandat 971.973 kb on - strandat 971.973 kb on - strandat 972.210 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline chloride 358 mM
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970,397 + -0.4
970,398 - +0.3
971,307 + +0.8
971,307 + -1.2
971,308 - -2.2
971,486 + AO353_04500 0.24 +0.4
971,486 + AO353_04500 0.24 -0.0
971,541 + AO353_04500 0.32 -2.0
971,669 + AO353_04500 0.51 -2.2
971,735 + AO353_04500 0.61 -2.0
971,736 - AO353_04500 0.61 +0.8
971,736 - AO353_04500 0.61 -1.2
971,941 - +0.4
971,941 - -0.8
971,961 + -0.5
971,972 + -0.2
971,973 - -0.2
971,973 - -0.1
971,973 - -1.5
971,973 - +1.8
971,973 - -0.2
972,210 - -0.4

Or see this region's nucleotide sequence