Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfC and Synpcc7942_2366 are separated by 91 nucleotides Synpcc7942_2366 and Synpcc7942_2367 are separated by 71 nucleotides Synpcc7942_2367 and tRNA-His are separated by 38 nucleotides tRNA-His and Synpcc7942_2368 are separated by 321 nucleotides
Synpcc7942_2365: prfC - peptide chain release factor 3, at 2,430,839 to 2,432,509
prfC
Synpcc7942_2366: Synpcc7942_2366 - hypothetical protein, at 2,432,601 to 2,432,879
_2366
Synpcc7942_2367: Synpcc7942_2367 - hypothetical protein, at 2,432,951 to 2,433,454
_2367
Synpcc7942_R0013: tRNA-His - tRNA-His, at 2,433,493 to 2,433,568
tRNA-His
Synpcc7942_2368: Synpcc7942_2368 - Secretion protein HlyD, at 2,433,890 to 2,435,020
_2368
Position (kb)
2432
2433
2434 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2431.960 kb on - strand, within prfC at 2431.971 kb on - strand, within prfC at 2431.987 kb on - strand, within prfC at 2431.994 kb on - strand, within prfC at 2432.041 kb on + strand, within prfC at 2432.045 kb on + strand, within prfC at 2432.097 kb on + strand, within prfC at 2432.105 kb on - strand, within prfC at 2432.113 kb on + strand, within prfC at 2432.121 kb on - strand, within prfC at 2432.125 kb on + strand, within prfC at 2432.128 kb on - strand, within prfC at 2432.136 kb on + strand, within prfC at 2432.152 kb on + strand, within prfC at 2432.180 kb on + strand, within prfC at 2432.227 kb on - strand, within prfC at 2432.263 kb on - strand, within prfC at 2432.293 kb on + strand, within prfC at 2432.313 kb on + strand, within prfC at 2432.315 kb on + strand, within prfC at 2432.353 kb on + strand at 2432.356 kb on + strand at 2432.371 kb on + strand at 2432.380 kb on + strand at 2432.380 kb on + strand at 2432.396 kb on + strand at 2432.396 kb on + strand at 2432.415 kb on + strand at 2432.512 kb on - strand at 2432.515 kb on + strand at 2432.515 kb on + strand at 2432.523 kb on - strand at 2432.541 kb on + strand at 2432.544 kb on + strand at 2432.594 kb on - strand at 2432.601 kb on + strand at 2432.601 kb on - strand at 2432.606 kb on - strand at 2432.606 kb on - strand at 2432.609 kb on - strand at 2432.645 kb on - strand, within Synpcc7942_2366 at 2432.663 kb on + strand, within Synpcc7942_2366 at 2432.697 kb on + strand, within Synpcc7942_2366 at 2432.699 kb on + strand, within Synpcc7942_2366 at 2432.746 kb on - strand, within Synpcc7942_2366 at 2432.748 kb on + strand, within Synpcc7942_2366 at 2432.754 kb on + strand, within Synpcc7942_2366 at 2432.754 kb on + strand, within Synpcc7942_2366 at 2432.754 kb on + strand, within Synpcc7942_2366 at 2432.754 kb on + strand, within Synpcc7942_2366 at 2432.756 kb on - strand, within Synpcc7942_2366 at 2432.756 kb on - strand, within Synpcc7942_2366 at 2432.762 kb on - strand, within Synpcc7942_2366 at 2432.762 kb on - strand at 2432.787 kb on - strand, within Synpcc7942_2366 at 2432.787 kb on - strand at 2432.793 kb on - strand, within Synpcc7942_2366 at 2432.795 kb on - strand, within Synpcc7942_2366 at 2432.856 kb on - strand at 2432.908 kb on + strand at 2432.908 kb on + strand at 2432.910 kb on + strand at 2432.916 kb on - strand at 2432.956 kb on + strand at 2432.964 kb on - strand at 2432.964 kb on - strand at 2432.964 kb on - strand at 2432.964 kb on - strand at 2432.985 kb on + strand at 2432.985 kb on + strand at 2433.019 kb on + strand, within Synpcc7942_2367 at 2433.034 kb on - strand, within Synpcc7942_2367 at 2433.053 kb on + strand, within Synpcc7942_2367 at 2433.053 kb on - strand, within Synpcc7942_2367 at 2433.059 kb on - strand, within Synpcc7942_2367 at 2433.059 kb on - strand, within Synpcc7942_2367 at 2433.066 kb on - strand, within Synpcc7942_2367 at 2433.079 kb on + strand at 2433.080 kb on + strand, within Synpcc7942_2367 at 2433.117 kb on + strand at 2433.138 kb on + strand, within Synpcc7942_2367 at 2433.143 kb on + strand, within Synpcc7942_2367 at 2433.151 kb on - strand, within Synpcc7942_2367 at 2433.151 kb on - strand, within Synpcc7942_2367 at 2433.194 kb on + strand, within Synpcc7942_2367 at 2433.194 kb on + strand, within Synpcc7942_2367 at 2433.220 kb on + strand, within Synpcc7942_2367 at 2433.220 kb on + strand, within Synpcc7942_2367 at 2433.228 kb on - strand, within Synpcc7942_2367 at 2433.228 kb on - strand, within Synpcc7942_2367 at 2433.228 kb on - strand, within Synpcc7942_2367 at 2433.245 kb on + strand, within Synpcc7942_2367 at 2433.329 kb on + strand, within Synpcc7942_2367 at 2433.329 kb on + strand, within Synpcc7942_2367 at 2433.337 kb on - strand, within Synpcc7942_2367 at 2433.343 kb on - strand, within Synpcc7942_2367 at 2433.343 kb on - strand, within Synpcc7942_2367 at 2433.355 kb on + strand, within Synpcc7942_2367 at 2433.358 kb on - strand, within Synpcc7942_2367 at 2433.395 kb on + strand, within Synpcc7942_2367 at 2433.443 kb on - strand at 2433.479 kb on - strand at 2433.632 kb on - strand at 2433.651 kb on - strand at 2433.707 kb on + strand at 2433.715 kb on - strand at 2433.717 kb on + strand at 2433.755 kb on + strand at 2433.755 kb on + strand at 2433.755 kb on + strand at 2433.762 kb on - strand at 2433.763 kb on - strand at 2433.763 kb on - strand at 2433.763 kb on - strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.765 kb on + strand at 2433.767 kb on + strand at 2433.767 kb on + strand at 2433.767 kb on + strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.773 kb on - strand at 2433.775 kb on - strand at 2433.797 kb on - strand at 2433.819 kb on + strand at 2433.867 kb on - strand at 2433.869 kb on - strand at 2433.875 kb on + strand at 2433.875 kb on + strand at 2433.880 kb on + strand at 2433.883 kb on - strand at 2433.896 kb on + strand at 2433.898 kb on + strand at 2433.906 kb on - strand at 2433.906 kb on - strand at 2433.928 kb on + strand at 2433.950 kb on + strand at 2433.977 kb on + strand at 2434.003 kb on - strand, within Synpcc7942_2368 at 2434.058 kb on - strand, within Synpcc7942_2368 at 2434.103 kb on - strand, within Synpcc7942_2368 at 2434.151 kb on + strand, within Synpcc7942_2368 at 2434.273 kb on - strand, within Synpcc7942_2368 at 2434.302 kb on + strand, within Synpcc7942_2368 at 2434.326 kb on + strand, within Synpcc7942_2368 at 2434.348 kb on + strand, within Synpcc7942_2368 at 2434.356 kb on - strand, within Synpcc7942_2368 at 2434.367 kb on - strand, within Synpcc7942_2368 at 2434.369 kb on + strand, within Synpcc7942_2368 at 2434.377 kb on - strand, within Synpcc7942_2368 at 2434.377 kb on - strand, within Synpcc7942_2368 at 2434.377 kb on - strand, within Synpcc7942_2368 at 2434.417 kb on + strand, within Synpcc7942_2368 at 2434.425 kb on - strand, within Synpcc7942_2368
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 2,431,960 - prfC Synpcc7942_2365 0.67 -1.7 2,431,971 - prfC Synpcc7942_2365 0.68 +1.7 2,431,987 - prfC Synpcc7942_2365 0.69 -2.7 2,431,994 - prfC Synpcc7942_2365 0.69 -2.9 2,432,041 + prfC Synpcc7942_2365 0.72 -1.3 2,432,045 + prfC Synpcc7942_2365 0.72 -0.1 2,432,097 + prfC Synpcc7942_2365 0.75 -3.7 2,432,105 - prfC Synpcc7942_2365 0.76 -3.1 2,432,113 + prfC Synpcc7942_2365 0.76 -0.8 2,432,121 - prfC Synpcc7942_2365 0.77 -0.1 2,432,125 + prfC Synpcc7942_2365 0.77 -2.7 2,432,128 - prfC Synpcc7942_2365 0.77 -4.4 2,432,136 + prfC Synpcc7942_2365 0.78 -2.7 2,432,152 + prfC Synpcc7942_2365 0.79 -2.1 2,432,180 + prfC Synpcc7942_2365 0.80 -0.1 2,432,227 - prfC Synpcc7942_2365 0.83 -2.4 2,432,263 - prfC Synpcc7942_2365 0.85 -0.1 2,432,293 + prfC Synpcc7942_2365 0.87 -1.7 2,432,313 + prfC Synpcc7942_2365 0.88 -1.7 2,432,315 + prfC Synpcc7942_2365 0.88 -2.7 2,432,353 + -1.1 2,432,356 + -0.5 2,432,371 + -1.7 2,432,380 + -1.7 2,432,380 + -1.4 2,432,396 + -2.4 2,432,396 + -1.7 2,432,415 + -1.4 2,432,512 - +0.8 2,432,515 + -0.1 2,432,515 + +0.7 2,432,523 - -0.3 2,432,541 + -0.1 2,432,544 + +1.9 2,432,594 - +0.2 2,432,601 + +1.2 2,432,601 - -0.3 2,432,606 - -3.4 2,432,606 - +0.2 2,432,609 - +0.8 2,432,645 - Synpcc7942_2366 0.16 -0.1 2,432,663 + Synpcc7942_2366 0.22 -0.1 2,432,697 + Synpcc7942_2366 0.34 +0.4 2,432,699 + Synpcc7942_2366 0.35 +0.5 2,432,746 - Synpcc7942_2366 0.52 +0.2 2,432,748 + Synpcc7942_2366 0.53 -0.1 2,432,754 + Synpcc7942_2366 0.55 -1.9 2,432,754 + Synpcc7942_2366 0.55 +1.1 2,432,754 + Synpcc7942_2366 0.55 -0.7 2,432,754 + Synpcc7942_2366 0.55 +1.2 2,432,756 - Synpcc7942_2366 0.56 +0.2 2,432,756 - Synpcc7942_2366 0.56 +1.5 2,432,762 - Synpcc7942_2366 0.58 -0.5 2,432,762 - -0.1 2,432,787 - Synpcc7942_2366 0.67 +1.6 2,432,787 - +0.5 2,432,793 - Synpcc7942_2366 0.69 -0.6 2,432,795 - Synpcc7942_2366 0.70 -0.9 2,432,856 - +1.5 2,432,908 + -0.9 2,432,908 + +0.4 2,432,910 + +0.3 2,432,916 - -0.1 2,432,956 + -1.7 2,432,964 - -0.7 2,432,964 - -0.2 2,432,964 - -1.2 2,432,964 - -1.1 2,432,985 + +0.0 2,432,985 + -0.1 2,433,019 + Synpcc7942_2367 0.13 -2.4 2,433,034 - Synpcc7942_2367 0.16 -0.2 2,433,053 + Synpcc7942_2367 0.20 -1.3 2,433,053 - Synpcc7942_2367 0.20 -0.7 2,433,059 - Synpcc7942_2367 0.21 -3.3 2,433,059 - Synpcc7942_2367 0.21 -2.7 2,433,066 - Synpcc7942_2367 0.23 -1.5 2,433,079 + -0.5 2,433,080 + Synpcc7942_2367 0.26 +1.9 2,433,117 + -0.7 2,433,138 + Synpcc7942_2367 0.37 -2.9 2,433,143 + Synpcc7942_2367 0.38 -2.1 2,433,151 - Synpcc7942_2367 0.40 -0.7 2,433,151 - Synpcc7942_2367 0.40 -0.9 2,433,194 + Synpcc7942_2367 0.48 -2.6 2,433,194 + Synpcc7942_2367 0.48 -3.1 2,433,220 + Synpcc7942_2367 0.53 +0.0 2,433,220 + Synpcc7942_2367 0.53 -0.8 2,433,228 - Synpcc7942_2367 0.55 -0.3 2,433,228 - Synpcc7942_2367 0.55 -1.7 2,433,228 - Synpcc7942_2367 0.55 +0.1 2,433,245 + Synpcc7942_2367 0.58 -1.7 2,433,329 + Synpcc7942_2367 0.75 -2.0 2,433,329 + Synpcc7942_2367 0.75 -2.4 2,433,337 - Synpcc7942_2367 0.77 -0.5 2,433,343 - Synpcc7942_2367 0.78 -0.7 2,433,343 - Synpcc7942_2367 0.78 -0.8 2,433,355 + Synpcc7942_2367 0.80 -1.8 2,433,358 - Synpcc7942_2367 0.81 -2.9 2,433,395 + Synpcc7942_2367 0.88 -0.2 2,433,443 - -0.4 2,433,479 - -1.2 2,433,632 - +0.2 2,433,651 - -1.9 2,433,707 + -1.1 2,433,715 - -2.4 2,433,717 + +2.1 2,433,755 + -0.5 2,433,755 + +1.2 2,433,755 + +0.1 2,433,762 - -0.0 2,433,763 - -0.6 2,433,763 - -0.7 2,433,763 - -2.1 2,433,765 + +1.1 2,433,765 + -0.5 2,433,765 + -2.9 2,433,765 + +1.0 2,433,765 + +0.9 2,433,765 + -1.1 2,433,765 + +1.1 2,433,765 + -0.7 2,433,765 + +0.6 2,433,765 + +0.2 2,433,765 + +1.1 2,433,765 + -0.5 2,433,765 + +0.7 2,433,765 + -0.1 2,433,765 + +0.1 2,433,765 + -0.9 2,433,765 + -0.5 2,433,765 + -3.0 2,433,765 + +0.1 2,433,765 + -0.3 2,433,765 + -2.4 2,433,767 + +1.9 2,433,767 + -0.8 2,433,767 + -1.2 2,433,773 - -0.4 2,433,773 - +1.1 2,433,773 - -0.3 2,433,773 - +0.2 2,433,773 - +1.2 2,433,773 - -1.8 2,433,773 - +1.5 2,433,773 - +0.9 2,433,773 - +1.6 2,433,773 - +1.9 2,433,773 - +1.2 2,433,773 - -0.1 2,433,773 - -1.1 2,433,773 - -0.4 2,433,773 - -0.7 2,433,773 - -0.1 2,433,773 - +0.0 2,433,773 - -0.4 2,433,773 - -1.7 2,433,773 - +0.3 2,433,773 - -2.1 2,433,775 - -1.4 2,433,797 - +0.6 2,433,819 + +0.2 2,433,867 - -0.2 2,433,869 - -1.2 2,433,875 + -0.4 2,433,875 + +0.9 2,433,880 + -0.1 2,433,883 - -1.3 2,433,896 + -0.8 2,433,898 + -0.1 2,433,906 - +0.8 2,433,906 - -1.4 2,433,928 + -2.2 2,433,950 + -0.1 2,433,977 + -0.3 2,434,003 - Synpcc7942_2368 0.10 -1.1 2,434,058 - Synpcc7942_2368 0.15 -1.5 2,434,103 - Synpcc7942_2368 0.19 -1.2 2,434,151 + Synpcc7942_2368 0.23 -0.1 2,434,273 - Synpcc7942_2368 0.34 -2.4 2,434,302 + Synpcc7942_2368 0.36 -0.1 2,434,326 + Synpcc7942_2368 0.39 +1.0 2,434,348 + Synpcc7942_2368 0.40 +0.3 2,434,356 - Synpcc7942_2368 0.41 -0.1 2,434,367 - Synpcc7942_2368 0.42 -0.1 2,434,369 + Synpcc7942_2368 0.42 -0.3 2,434,377 - Synpcc7942_2368 0.43 +0.2 2,434,377 - Synpcc7942_2368 0.43 -0.7 2,434,377 - Synpcc7942_2368 0.43 +0.7 2,434,417 + Synpcc7942_2368 0.47 +0.5 2,434,425 - Synpcc7942_2368 0.47 +0.1
Or see this region's nucleotide sequence