Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrC and lepB overlap by 45 nucleotides lepB and Synpcc7942_0488 are separated by 143 nucleotides Synpcc7942_0488 and aldA are separated by 20 nucleotides
Synpcc7942_0486: pyrC - dihydroorotase, at 472,319 to 473,554
pyrC
Synpcc7942_0487: lepB - thylakoidal processing peptidase. Serine peptidase. MEROPS family S26A, at 473,510 to 474,172
lepB
Synpcc7942_0488: Synpcc7942_0488 - dihydroorotase, at 474,316 to 475,644
_0488
Synpcc7942_0489: aldA - aldehyde dehydrogenase, at 475,665 to 477,044
aldA
Position (kb) 474
475
476 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 473.533 kb on + strand at 473.549 kb on + strand at 473.549 kb on + strand at 473.549 kb on + strand at 473.600 kb on - strand, within lepB at 474.176 kb on + strand at 474.222 kb on - strand at 474.277 kb on + strand at 474.283 kb on + strand at 474.294 kb on - strand at 475.663 kb on + strand at 475.663 kb on - strand at 475.710 kb on + strand at 475.713 kb on - strand at 475.723 kb on - strand at 475.724 kb on - strand at 475.750 kb on + strand at 475.758 kb on - strand at 475.760 kb on + strand at 475.890 kb on - strand, within aldA at 475.905 kb on + strand, within aldA at 475.913 kb on - strand, within aldA at 475.913 kb on - strand, within aldA at 475.926 kb on + strand, within aldA at 475.934 kb on - strand, within aldA at 475.948 kb on + strand, within aldA at 475.948 kb on + strand, within aldA at 475.966 kb on - strand, within aldA at 476.078 kb on + strand, within aldA at 476.103 kb on + strand, within aldA at 476.103 kb on - strand, within aldA at 476.152 kb on - strand, within aldA at 476.162 kb on + strand, within aldA at 476.186 kb on + strand, within aldA at 476.194 kb on - strand, within aldA at 476.212 kb on + strand, within aldA at 476.212 kb on + strand, within aldA at 476.220 kb on - strand, within aldA at 476.220 kb on - strand, within aldA at 476.220 kb on - strand, within aldA at 476.222 kb on + strand, within aldA at 476.255 kb on + strand, within aldA at 476.268 kb on + strand, within aldA at 476.276 kb on - strand, within aldA at 476.286 kb on - strand, within aldA at 476.343 kb on - strand, within aldA at 476.347 kb on + strand, within aldA at 476.372 kb on + strand, within aldA at 476.380 kb on - strand, within aldA at 476.385 kb on - strand, within aldA at 476.399 kb on + strand, within aldA at 476.421 kb on + strand, within aldA at 476.478 kb on + strand, within aldA at 476.495 kb on + strand, within aldA at 476.498 kb on - strand, within aldA at 476.507 kb on - strand, within aldA at 476.541 kb on + strand, within aldA at 476.601 kb on + strand, within aldA at 476.614 kb on + strand, within aldA at 476.622 kb on - strand, within aldA at 476.637 kb on - strand, within aldA
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 473,533 + +0.2 473,549 + +1.9 473,549 + -0.7 473,549 + -0.1 473,600 - lepB Synpcc7942_0487 0.14 -2.8 474,176 + -2.1 474,222 - +0.8 474,277 + -1.7 474,283 + -0.8 474,294 - -2.9 475,663 + +1.5 475,663 - +0.9 475,710 + -0.1 475,713 - -0.1 475,723 - -0.7 475,724 - +0.7 475,750 + -1.7 475,758 - +0.0 475,760 + -1.7 475,890 - aldA Synpcc7942_0489 0.16 +0.6 475,905 + aldA Synpcc7942_0489 0.17 +0.1 475,913 - aldA Synpcc7942_0489 0.18 -1.4 475,913 - aldA Synpcc7942_0489 0.18 -1.1 475,926 + aldA Synpcc7942_0489 0.19 -0.1 475,934 - aldA Synpcc7942_0489 0.19 +0.5 475,948 + aldA Synpcc7942_0489 0.21 -1.8 475,948 + aldA Synpcc7942_0489 0.21 -4.2 475,966 - aldA Synpcc7942_0489 0.22 -0.1 476,078 + aldA Synpcc7942_0489 0.30 -0.1 476,103 + aldA Synpcc7942_0489 0.32 -1.1 476,103 - aldA Synpcc7942_0489 0.32 -2.1 476,152 - aldA Synpcc7942_0489 0.35 -0.8 476,162 + aldA Synpcc7942_0489 0.36 -1.7 476,186 + aldA Synpcc7942_0489 0.38 +0.2 476,194 - aldA Synpcc7942_0489 0.38 -1.7 476,212 + aldA Synpcc7942_0489 0.40 -2.0 476,212 + aldA Synpcc7942_0489 0.40 -1.7 476,220 - aldA Synpcc7942_0489 0.40 -2.1 476,220 - aldA Synpcc7942_0489 0.40 -2.4 476,220 - aldA Synpcc7942_0489 0.40 -2.9 476,222 + aldA Synpcc7942_0489 0.40 -0.1 476,255 + aldA Synpcc7942_0489 0.43 -0.1 476,268 + aldA Synpcc7942_0489 0.44 +0.6 476,276 - aldA Synpcc7942_0489 0.44 -0.1 476,286 - aldA Synpcc7942_0489 0.45 -1.1 476,343 - aldA Synpcc7942_0489 0.49 -0.1 476,347 + aldA Synpcc7942_0489 0.49 -0.1 476,372 + aldA Synpcc7942_0489 0.51 -1.7 476,380 - aldA Synpcc7942_0489 0.52 -1.1 476,385 - aldA Synpcc7942_0489 0.52 -1.2 476,399 + aldA Synpcc7942_0489 0.53 -0.1 476,421 + aldA Synpcc7942_0489 0.55 -0.1 476,478 + aldA Synpcc7942_0489 0.59 -2.4 476,495 + aldA Synpcc7942_0489 0.60 -2.4 476,498 - aldA Synpcc7942_0489 0.60 -0.5 476,507 - aldA Synpcc7942_0489 0.61 -0.1 476,541 + aldA Synpcc7942_0489 0.63 -1.1 476,601 + aldA Synpcc7942_0489 0.68 -1.1 476,614 + aldA Synpcc7942_0489 0.69 +0.3 476,622 - aldA Synpcc7942_0489 0.69 +0.9 476,637 - aldA Synpcc7942_0489 0.70 -1.1
Or see this region's nucleotide sequence