Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0451 and Synpcc7942_0452 are separated by 57 nucleotides Synpcc7942_0452 and Synpcc7942_0453 are separated by 185 nucleotides Synpcc7942_0453 and cobS overlap by 20 nucleotides cobS and tgt are separated by 28 nucleotides
Synpcc7942_0451: Synpcc7942_0451 - esterase, at 440,670 to 441,299
_0451
Synpcc7942_0452: Synpcc7942_0452 - hypothetical protein, at 441,357 to 441,836
_0452
Synpcc7942_0453: Synpcc7942_0453 - GAF sensor signal transduction histidine kinase, at 442,022 to 443,236
_0453
Synpcc7942_0454: cobS - cobalamin synthase, at 443,217 to 443,972
cobS
Synpcc7942_0455: tgt - queuine tRNA-ribosyltransferase, at 444,001 to 445,155
tgt
Position (kb)
442
443
444 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 441.039 kb on - strand, within Synpcc7942_0451 at 441.049 kb on - strand, within Synpcc7942_0451 at 441.068 kb on + strand, within Synpcc7942_0451 at 441.074 kb on + strand, within Synpcc7942_0451 at 441.074 kb on + strand, within Synpcc7942_0451 at 441.082 kb on - strand, within Synpcc7942_0451 at 441.082 kb on - strand, within Synpcc7942_0451 at 441.106 kb on + strand, within Synpcc7942_0451 at 441.144 kb on + strand, within Synpcc7942_0451 at 441.194 kb on + strand, within Synpcc7942_0451 at 441.196 kb on + strand, within Synpcc7942_0451 at 441.202 kb on - strand, within Synpcc7942_0451 at 441.204 kb on - strand, within Synpcc7942_0451 at 441.204 kb on - strand, within Synpcc7942_0451 at 441.204 kb on - strand, within Synpcc7942_0451 at 441.209 kb on - strand, within Synpcc7942_0451 at 441.235 kb on + strand, within Synpcc7942_0451 at 441.272 kb on + strand at 441.280 kb on - strand at 441.440 kb on + strand, within Synpcc7942_0452 at 441.505 kb on + strand, within Synpcc7942_0452 at 441.573 kb on - strand, within Synpcc7942_0452 at 441.765 kb on + strand, within Synpcc7942_0452 at 441.860 kb on + strand at 441.915 kb on + strand at 441.924 kb on + strand at 441.924 kb on + strand at 441.936 kb on + strand at 441.937 kb on + strand at 441.967 kb on - strand at 441.991 kb on - strand at 441.992 kb on + strand at 442.004 kb on + strand at 442.012 kb on - strand at 443.209 kb on + strand at 443.215 kb on + strand at 443.215 kb on + strand at 443.953 kb on + strand at 443.953 kb on + strand at 443.961 kb on - strand at 443.961 kb on - strand at 443.961 kb on - strand at 443.961 kb on - strand at 443.961 kb on - strand at 443.961 kb on - strand at 443.961 kb on - strand at 443.961 kb on - strand at 443.961 kb on - strand at 443.981 kb on + strand at 444.004 kb on + strand at 444.017 kb on - strand at 444.042 kb on + strand at 444.042 kb on - strand at 444.070 kb on + strand at 444.073 kb on - strand at 444.073 kb on - strand at 444.096 kb on - strand at 444.107 kb on + strand at 444.207 kb on + strand, within tgt at 444.207 kb on + strand, within tgt at 444.207 kb on + strand, within tgt at 444.207 kb on + strand, within tgt at 444.215 kb on - strand, within tgt at 444.215 kb on - strand, within tgt at 444.215 kb on - strand, within tgt at 444.221 kb on - strand, within tgt
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 441,039 - Synpcc7942_0451 0.59 +1.0 441,049 - Synpcc7942_0451 0.60 -2.4 441,068 + Synpcc7942_0451 0.63 -0.3 441,074 + Synpcc7942_0451 0.64 +0.2 441,074 + Synpcc7942_0451 0.64 +0.8 441,082 - Synpcc7942_0451 0.65 +2.0 441,082 - Synpcc7942_0451 0.65 +1.4 441,106 + Synpcc7942_0451 0.69 +2.3 441,144 + Synpcc7942_0451 0.75 -0.0 441,194 + Synpcc7942_0451 0.83 +2.0 441,196 + Synpcc7942_0451 0.83 +0.1 441,202 - Synpcc7942_0451 0.84 +0.2 441,204 - Synpcc7942_0451 0.85 -0.0 441,204 - Synpcc7942_0451 0.85 +0.2 441,204 - Synpcc7942_0451 0.85 +1.5 441,209 - Synpcc7942_0451 0.86 +1.4 441,235 + Synpcc7942_0451 0.90 -1.6 441,272 + +0.2 441,280 - -0.5 441,440 + Synpcc7942_0452 0.17 +0.1 441,505 + Synpcc7942_0452 0.31 -2.0 441,573 - Synpcc7942_0452 0.45 -1.9 441,765 + Synpcc7942_0452 0.85 -1.6 441,860 + -1.0 441,915 + +1.0 441,924 + -0.7 441,924 + +0.8 441,936 + +0.5 441,937 + -0.0 441,967 - +0.1 441,991 - +0.3 441,992 + -0.6 442,004 + -0.0 442,012 - +0.8 443,209 + +0.3 443,215 + +0.7 443,215 + -0.6 443,953 + -0.9 443,953 + -0.2 443,961 - -1.6 443,961 - -1.6 443,961 - -0.2 443,961 - +0.9 443,961 - -1.6 443,961 - -0.0 443,961 - +1.8 443,961 - +0.1 443,961 - +2.0 443,981 + +0.1 444,004 + -0.6 444,017 - -0.3 444,042 + -0.3 444,042 - -0.9 444,070 + -3.5 444,073 - -4.0 444,073 - -2.6 444,096 - -3.9 444,107 + -1.9 444,207 + tgt Synpcc7942_0455 0.18 -4.9 444,207 + tgt Synpcc7942_0455 0.18 -3.2 444,207 + tgt Synpcc7942_0455 0.18 -3.7 444,207 + tgt Synpcc7942_0455 0.18 -2.3 444,215 - tgt Synpcc7942_0455 0.19 -2.7 444,215 - tgt Synpcc7942_0455 0.19 -6.0 444,215 - tgt Synpcc7942_0455 0.19 -4.2 444,221 - tgt Synpcc7942_0455 0.19 -5.3
Or see this region's nucleotide sequence