Experiment: Recovery broth with EDTA 1.0 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16605 and BBR_RS16610 are separated by 64 nucleotides BBR_RS16610 and BBR_RS16615 are separated by 11 nucleotides BBR_RS16615 and BBR_RS16620 overlap by 11 nucleotides BBR_RS16620 and BBR_RS16625 are separated by 15 nucleotides
BBR_RS16605: BBR_RS16605 - hypothetical protein, at 1,493,206 to 1,495,818
_RS16605
BBR_RS16610: BBR_RS16610 - hypothetical protein, at 1,495,883 to 1,496,467
_RS16610
BBR_RS16615: BBR_RS16615 - class I SAM-dependent RNA methyltransferase, at 1,496,479 to 1,497,738
_RS16615
BBR_RS16620: BBR_RS16620 - DUF3159 domain-containing protein, at 1,497,728 to 1,498,504
_RS16620
BBR_RS16625: BBR_RS16625 - DUF3710 domain-containing protein, at 1,498,520 to 1,499,365
_RS16625
Position (kb)
1496
1497
1498 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1495.517 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.538 kb on + strand, within BBR_RS16605 at 1495.546 kb on - strand, within BBR_RS16605 at 1495.660 kb on - strand at 1495.687 kb on + strand at 1495.704 kb on + strand at 1495.772 kb on - strand at 1495.816 kb on + strand at 1495.819 kb on - strand at 1495.910 kb on + strand at 1495.910 kb on + strand at 1495.962 kb on + strand, within BBR_RS16610 at 1496.003 kb on + strand, within BBR_RS16610 at 1496.009 kb on - strand, within BBR_RS16610 at 1496.023 kb on - strand, within BBR_RS16610 at 1496.053 kb on - strand, within BBR_RS16610 at 1496.075 kb on + strand, within BBR_RS16610 at 1496.086 kb on + strand, within BBR_RS16610 at 1496.104 kb on - strand, within BBR_RS16610 at 1496.235 kb on + strand, within BBR_RS16610 at 1496.253 kb on - strand, within BBR_RS16610 at 1496.377 kb on - strand, within BBR_RS16610 at 1496.412 kb on + strand at 1496.472 kb on - strand at 1496.475 kb on + strand at 1496.561 kb on - strand at 1496.565 kb on + strand at 1496.569 kb on + strand at 1496.623 kb on - strand, within BBR_RS16615 at 1496.689 kb on + strand, within BBR_RS16615 at 1496.719 kb on + strand, within BBR_RS16615 at 1496.719 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.741 kb on + strand, within BBR_RS16615 at 1496.749 kb on - strand, within BBR_RS16615 at 1496.749 kb on - strand, within BBR_RS16615 at 1496.759 kb on - strand, within BBR_RS16615 at 1496.823 kb on + strand, within BBR_RS16615 at 1496.823 kb on + strand, within BBR_RS16615 at 1496.850 kb on - strand, within BBR_RS16615 at 1496.856 kb on + strand, within BBR_RS16615 at 1496.875 kb on - strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.917 kb on + strand, within BBR_RS16615 at 1496.936 kb on + strand, within BBR_RS16615 at 1496.944 kb on - strand, within BBR_RS16615 at 1496.944 kb on - strand, within BBR_RS16615 at 1496.969 kb on + strand, within BBR_RS16615 at 1497.014 kb on + strand, within BBR_RS16615 at 1497.060 kb on + strand, within BBR_RS16615 at 1497.075 kb on + strand, within BBR_RS16615 at 1497.091 kb on - strand, within BBR_RS16615 at 1497.145 kb on + strand, within BBR_RS16615 at 1497.155 kb on + strand, within BBR_RS16615 at 1497.191 kb on - strand, within BBR_RS16615 at 1497.214 kb on - strand, within BBR_RS16615 at 1497.264 kb on - strand, within BBR_RS16615 at 1497.312 kb on + strand, within BBR_RS16615 at 1497.348 kb on - strand, within BBR_RS16615 at 1497.412 kb on - strand, within BBR_RS16615 at 1497.497 kb on + strand, within BBR_RS16615 at 1497.527 kb on + strand, within BBR_RS16615 at 1497.535 kb on - strand, within BBR_RS16615 at 1497.555 kb on - strand, within BBR_RS16615 at 1497.584 kb on + strand, within BBR_RS16615 at 1497.584 kb on + strand, within BBR_RS16615 at 1497.621 kb on + strand at 1497.629 kb on - strand at 1497.681 kb on - strand at 1497.692 kb on - strand at 1497.697 kb on + strand at 1497.746 kb on - strand at 1497.903 kb on + strand, within BBR_RS16620 at 1497.911 kb on - strand, within BBR_RS16620 at 1497.925 kb on - strand, within BBR_RS16620 at 1497.959 kb on + strand, within BBR_RS16620 at 1498.012 kb on - strand, within BBR_RS16620 at 1498.012 kb on - strand, within BBR_RS16620 at 1498.041 kb on - strand, within BBR_RS16620 at 1498.116 kb on - strand, within BBR_RS16620 at 1498.193 kb on - strand, within BBR_RS16620 at 1498.221 kb on - strand, within BBR_RS16620 at 1498.240 kb on - strand, within BBR_RS16620 at 1498.267 kb on + strand, within BBR_RS16620 at 1498.388 kb on - strand, within BBR_RS16620 at 1498.483 kb on - strand at 1498.513 kb on + strand at 1498.596 kb on + strand at 1498.646 kb on - strand, within BBR_RS16625 at 1498.727 kb on - strand, within BBR_RS16625
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with EDTA 1.0 mM remove 1,495,517 + BBR_RS16605 0.88 -1.9 1,495,538 + BBR_RS16605 0.89 -0.9 1,495,538 + BBR_RS16605 0.89 -0.4 1,495,538 + BBR_RS16605 0.89 -0.1 1,495,546 - BBR_RS16605 0.90 -1.1 1,495,660 - -2.1 1,495,687 + -2.3 1,495,704 + -1.0 1,495,772 - -2.9 1,495,816 + +0.5 1,495,819 - -0.1 1,495,910 + -0.1 1,495,910 + +0.1 1,495,962 + BBR_RS16610 0.14 +0.2 1,496,003 + BBR_RS16610 0.21 -1.1 1,496,009 - BBR_RS16610 0.22 +0.4 1,496,023 - BBR_RS16610 0.24 -1.6 1,496,053 - BBR_RS16610 0.29 -0.0 1,496,075 + BBR_RS16610 0.33 -1.5 1,496,086 + BBR_RS16610 0.35 -1.7 1,496,104 - BBR_RS16610 0.38 -0.6 1,496,235 + BBR_RS16610 0.60 -0.5 1,496,253 - BBR_RS16610 0.63 -1.0 1,496,377 - BBR_RS16610 0.84 -0.8 1,496,412 + -0.9 1,496,472 - +0.3 1,496,475 + +0.9 1,496,561 - +1.1 1,496,565 + +0.3 1,496,569 + -0.0 1,496,623 - BBR_RS16615 0.11 +0.2 1,496,689 + BBR_RS16615 0.17 -0.9 1,496,719 + BBR_RS16615 0.19 -1.5 1,496,719 + BBR_RS16615 0.19 -0.4 1,496,741 + BBR_RS16615 0.21 +0.2 1,496,741 + BBR_RS16615 0.21 -0.5 1,496,741 + BBR_RS16615 0.21 -0.6 1,496,749 - BBR_RS16615 0.21 -0.0 1,496,749 - BBR_RS16615 0.21 +0.2 1,496,759 - BBR_RS16615 0.22 -0.3 1,496,823 + BBR_RS16615 0.27 -0.4 1,496,823 + BBR_RS16615 0.27 +0.1 1,496,850 - BBR_RS16615 0.29 +0.4 1,496,856 + BBR_RS16615 0.30 -0.8 1,496,875 - BBR_RS16615 0.31 +0.2 1,496,917 + BBR_RS16615 0.35 -0.1 1,496,917 + BBR_RS16615 0.35 -1.4 1,496,917 + BBR_RS16615 0.35 -1.0 1,496,936 + BBR_RS16615 0.36 -0.0 1,496,944 - BBR_RS16615 0.37 -1.4 1,496,944 - BBR_RS16615 0.37 -0.3 1,496,969 + BBR_RS16615 0.39 +0.4 1,497,014 + BBR_RS16615 0.42 -0.9 1,497,060 + BBR_RS16615 0.46 -1.3 1,497,075 + BBR_RS16615 0.47 -1.0 1,497,091 - BBR_RS16615 0.49 +0.2 1,497,145 + BBR_RS16615 0.53 +1.0 1,497,155 + BBR_RS16615 0.54 +0.4 1,497,191 - BBR_RS16615 0.57 -0.5 1,497,214 - BBR_RS16615 0.58 -0.6 1,497,264 - BBR_RS16615 0.62 -1.5 1,497,312 + BBR_RS16615 0.66 -2.0 1,497,348 - BBR_RS16615 0.69 +0.2 1,497,412 - BBR_RS16615 0.74 +0.0 1,497,497 + BBR_RS16615 0.81 -1.7 1,497,527 + BBR_RS16615 0.83 -0.7 1,497,535 - BBR_RS16615 0.84 -0.2 1,497,555 - BBR_RS16615 0.85 -1.8 1,497,584 + BBR_RS16615 0.88 +0.9 1,497,584 + BBR_RS16615 0.88 -0.5 1,497,621 + -0.0 1,497,629 - -0.5 1,497,681 - -1.2 1,497,692 - -3.4 1,497,697 + -1.4 1,497,746 - +1.0 1,497,903 + BBR_RS16620 0.23 -0.3 1,497,911 - BBR_RS16620 0.24 +0.3 1,497,925 - BBR_RS16620 0.25 +1.2 1,497,959 + BBR_RS16620 0.30 -1.0 1,498,012 - BBR_RS16620 0.37 -0.4 1,498,012 - BBR_RS16620 0.37 -0.2 1,498,041 - BBR_RS16620 0.40 -0.1 1,498,116 - BBR_RS16620 0.50 +0.4 1,498,193 - BBR_RS16620 0.60 -0.5 1,498,221 - BBR_RS16620 0.63 +0.2 1,498,240 - BBR_RS16620 0.66 +0.1 1,498,267 + BBR_RS16620 0.69 -1.8 1,498,388 - BBR_RS16620 0.85 +0.7 1,498,483 - +0.1 1,498,513 + -0.4 1,498,596 + +0.0 1,498,646 - BBR_RS16625 0.15 -2.7 1,498,727 - BBR_RS16625 0.24 +0.3
Or see this region's nucleotide sequence