Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_216

Experiment: LB, pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_214 and Ac3H11_215 are separated by 19 nucleotidesAc3H11_215 and Ac3H11_216 are separated by 9 nucleotidesAc3H11_216 and Ac3H11_217 overlap by 4 nucleotides Ac3H11_214 - Ferredoxin reductase, at 26,300 to 27,595 _214 Ac3H11_215 - Probable ferredoxin subunit of a ring-hydroxylating dioxygenase oxidoreductase protein (EC 1.-.-.-), at 27,615 to 27,929 _215 Ac3H11_216 - Ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA, at 27,939 to 28,421 _216 Ac3H11_217 - Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB, at 28,418 to 29,743 _217 Position (kb) 27 28 29Strain fitness (log2 ratio) -2 -1 0 1at 26.962 kb on - strand, within Ac3H11_214at 27.232 kb on - strand, within Ac3H11_214at 27.661 kb on + strand, within Ac3H11_215at 27.835 kb on + strand, within Ac3H11_215at 27.836 kb on - strand, within Ac3H11_215at 27.949 kb on + strandat 27.949 kb on + strandat 27.949 kb on + strandat 27.949 kb on + strandat 28.099 kb on + strand, within Ac3H11_216at 28.099 kb on + strand, within Ac3H11_216at 28.099 kb on + strand, within Ac3H11_216at 28.099 kb on + strand, within Ac3H11_216at 28.099 kb on + strand, within Ac3H11_216at 28.100 kb on - strand, within Ac3H11_216at 28.100 kb on - strand, within Ac3H11_216at 28.100 kb on - strand, within Ac3H11_216at 28.100 kb on - strand, within Ac3H11_216at 28.174 kb on + strand, within Ac3H11_216at 28.174 kb on + strand, within Ac3H11_216at 28.175 kb on - strand, within Ac3H11_216at 28.175 kb on - strand, within Ac3H11_216at 28.177 kb on + strand, within Ac3H11_216at 28.177 kb on + strand, within Ac3H11_216at 28.211 kb on - strand, within Ac3H11_216at 28.300 kb on + strand, within Ac3H11_216at 28.301 kb on - strand, within Ac3H11_216at 28.375 kb on + strandat 28.396 kb on + strandat 28.397 kb on - strandat 28.397 kb on - strandat 28.397 kb on - strandat 28.449 kb on + strandat 28.455 kb on + strandat 28.455 kb on + strandat 28.455 kb on + strandat 28.455 kb on + strandat 28.455 kb on + strandat 28.455 kb on + strandat 28.456 kb on - strandat 28.456 kb on - strandat 28.456 kb on - strandat 29.082 kb on + strand, within Ac3H11_217at 29.083 kb on - strand, within Ac3H11_217at 29.142 kb on + strand, within Ac3H11_217at 29.142 kb on + strand, within Ac3H11_217at 29.143 kb on - strand, within Ac3H11_217at 29.143 kb on - strand, within Ac3H11_217at 29.187 kb on + strand, within Ac3H11_217at 29.397 kb on + strand, within Ac3H11_217at 29.397 kb on + strand, within Ac3H11_217at 29.397 kb on + strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.398 kb on - strand, within Ac3H11_217at 29.412 kb on + strand, within Ac3H11_217

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, pH9
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26,962 - Ac3H11_214 0.51 +0.3
27,232 - Ac3H11_214 0.72 +0.1
27,661 + Ac3H11_215 0.15 +0.4
27,835 + Ac3H11_215 0.70 +0.5
27,836 - Ac3H11_215 0.70 -0.6
27,949 + +0.9
27,949 + -0.4
27,949 + +1.2
27,949 + -0.5
28,099 + Ac3H11_216 0.33 -1.1
28,099 + Ac3H11_216 0.33 +0.6
28,099 + Ac3H11_216 0.33 -0.2
28,099 + Ac3H11_216 0.33 -1.0
28,099 + Ac3H11_216 0.33 +0.5
28,100 - Ac3H11_216 0.33 +1.5
28,100 - Ac3H11_216 0.33 +0.7
28,100 - Ac3H11_216 0.33 +1.0
28,100 - Ac3H11_216 0.33 -0.4
28,174 + Ac3H11_216 0.49 +0.0
28,174 + Ac3H11_216 0.49 -0.3
28,175 - Ac3H11_216 0.49 -0.1
28,175 - Ac3H11_216 0.49 -1.5
28,177 + Ac3H11_216 0.49 +0.3
28,177 + Ac3H11_216 0.49 +0.1
28,211 - Ac3H11_216 0.56 +0.6
28,300 + Ac3H11_216 0.75 +0.1
28,301 - Ac3H11_216 0.75 +0.4
28,375 + -0.5
28,396 + -0.1
28,397 - +0.6
28,397 - -1.2
28,397 - +0.5
28,449 + +0.4
28,455 + +0.9
28,455 + -0.8
28,455 + -0.2
28,455 + -0.1
28,455 + -0.1
28,455 + +0.5
28,456 - -2.0
28,456 - +1.2
28,456 - +1.6
29,082 + Ac3H11_217 0.50 -1.2
29,083 - Ac3H11_217 0.50 +0.7
29,142 + Ac3H11_217 0.55 +0.3
29,142 + Ac3H11_217 0.55 +0.1
29,143 - Ac3H11_217 0.55 -0.5
29,143 - Ac3H11_217 0.55 -0.4
29,187 + Ac3H11_217 0.58 +0.3
29,397 + Ac3H11_217 0.74 +0.4
29,397 + Ac3H11_217 0.74 +0.1
29,397 + Ac3H11_217 0.74 +0.3
29,398 - Ac3H11_217 0.74 -0.3
29,398 - Ac3H11_217 0.74 -0.1
29,398 - Ac3H11_217 0.74 +0.2
29,398 - Ac3H11_217 0.74 -0.2
29,398 - Ac3H11_217 0.74 +0.4
29,398 - Ac3H11_217 0.74 -0.0
29,398 - Ac3H11_217 0.74 -0.2
29,398 - Ac3H11_217 0.74 +0.9
29,398 - Ac3H11_217 0.74 +0.6
29,398 - Ac3H11_217 0.74 -0.1
29,398 - Ac3H11_217 0.74 +0.7
29,412 + Ac3H11_217 0.75 -0.1

Or see this region's nucleotide sequence