Experiment: LB, pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1868 and Ac3H11_1869 are separated by 49 nucleotides Ac3H11_1869 and Ac3H11_1870 are separated by 102 nucleotides Ac3H11_1870 and Ac3H11_1871 are separated by 22 nucleotides
Ac3H11_1868 - Glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 72,347 to 74,488
_1868
Ac3H11_1869 - D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.1.-); Histidinol-phosphatase (EC 3.1.3.15), at 74,538 to 75,149
_1869
Ac3H11_1870 - Phospholipid and glycerol acyltransferase (EC 2.3.1.51), at 75,252 to 76,001
_1870
Ac3H11_1871 - Putative predicted metal-dependent hydrolase, at 76,024 to 76,983
_1871
Position (kb)
74
75
76 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 74.501 kb on + strand at 74.501 kb on + strand at 74.502 kb on - strand at 74.502 kb on - strand at 74.502 kb on - strand at 74.502 kb on - strand at 74.537 kb on + strand at 74.537 kb on + strand at 74.537 kb on + strand at 74.538 kb on - strand at 74.538 kb on - strand at 74.800 kb on - strand, within Ac3H11_1869 at 75.017 kb on - strand, within Ac3H11_1869 at 75.017 kb on - strand, within Ac3H11_1869 at 75.200 kb on + strand at 75.999 kb on + strand at 75.999 kb on + strand at 75.999 kb on + strand at 75.999 kb on + strand at 75.999 kb on + strand at 75.999 kb on + strand at 76.000 kb on - strand at 76.000 kb on - strand at 76.000 kb on - strand at 76.000 kb on - strand at 76.000 kb on - strand at 76.104 kb on + strand at 76.148 kb on + strand, within Ac3H11_1871 at 76.149 kb on - strand, within Ac3H11_1871
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH9 remove 74,501 + +0.0 74,501 + +0.6 74,502 - -0.2 74,502 - -0.6 74,502 - +1.3 74,502 - -0.4 74,537 + -0.7 74,537 + -1.0 74,537 + -1.8 74,538 - +0.3 74,538 - -0.5 74,800 - Ac3H11_1869 0.43 -1.7 75,017 - Ac3H11_1869 0.78 +1.9 75,017 - Ac3H11_1869 0.78 +1.0 75,200 + +0.3 75,999 + +1.2 75,999 + +0.8 75,999 + +0.1 75,999 + +0.3 75,999 + -0.1 75,999 + -0.2 76,000 - -1.1 76,000 - -0.1 76,000 - +0.9 76,000 - -1.4 76,000 - +0.1 76,104 + +0.3 76,148 + Ac3H11_1871 0.13 +0.5 76,149 - Ac3H11_1871 0.13 -0.0
Or see this region's nucleotide sequence