Experiment: LB, pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_183 and Ac3H11_184 are separated by 135 nucleotides Ac3H11_184 and Ac3H11_185 are separated by 105 nucleotides Ac3H11_185 and Ac3H11_186 overlap by 12 nucleotides
Ac3H11_183 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 71,091 to 74,063
_183
Ac3H11_184 - hypothetical protein, at 74,199 to 74,573
_184
Ac3H11_185 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 74,679 to 76,364
_185
Ac3H11_186 - hypothetical protein, at 76,353 to 76,529
_186
Position (kb) 74
75
76
77 Strain fitness (log2 ratio) -2
-1
0
1
2 at 73.679 kb on - strand, within Ac3H11_183 at 73.679 kb on - strand, within Ac3H11_183 at 73.723 kb on + strand, within Ac3H11_183 at 73.723 kb on + strand, within Ac3H11_183 at 73.724 kb on - strand, within Ac3H11_183 at 73.927 kb on + strand at 73.927 kb on + strand at 73.927 kb on + strand at 73.927 kb on + strand at 73.927 kb on + strand at 73.928 kb on - strand at 73.928 kb on - strand at 73.983 kb on + strand at 74.047 kb on + strand at 74.048 kb on - strand at 74.048 kb on - strand at 74.101 kb on + strand at 74.155 kb on + strand at 74.155 kb on + strand at 74.155 kb on + strand at 74.155 kb on + strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.434 kb on + strand, within Ac3H11_184 at 74.434 kb on + strand, within Ac3H11_184 at 74.776 kb on + strand at 74.776 kb on + strand at 74.776 kb on + strand at 74.776 kb on + strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 75.013 kb on + strand, within Ac3H11_185 at 75.013 kb on + strand, within Ac3H11_185 at 75.113 kb on - strand, within Ac3H11_185 at 75.296 kb on - strand, within Ac3H11_185 at 75.307 kb on + strand, within Ac3H11_185 at 75.308 kb on - strand, within Ac3H11_185 at 75.319 kb on + strand, within Ac3H11_185 at 75.320 kb on - strand, within Ac3H11_185 at 75.379 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.541 kb on + strand, within Ac3H11_185 at 75.541 kb on + strand, within Ac3H11_185 at 75.541 kb on + strand, within Ac3H11_185 at 75.542 kb on - strand, within Ac3H11_185 at 75.542 kb on - strand, within Ac3H11_185 at 75.542 kb on - strand, within Ac3H11_185 at 75.542 kb on - strand, within Ac3H11_185 at 75.659 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.716 kb on - strand, within Ac3H11_185 at 75.716 kb on - strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.028 kb on - strand, within Ac3H11_185 at 76.028 kb on - strand, within Ac3H11_185 at 76.028 kb on - strand, within Ac3H11_185 at 76.082 kb on + strand, within Ac3H11_185 at 76.082 kb on + strand, within Ac3H11_185 at 76.083 kb on - strand, within Ac3H11_185 at 76.083 kb on - strand, within Ac3H11_185 at 76.219 kb on + strand at 76.219 kb on + strand at 76.219 kb on + strand at 76.219 kb on + strand at 76.220 kb on - strand at 76.220 kb on - strand at 76.220 kb on - strand at 76.220 kb on - strand at 76.364 kb on + strand at 76.365 kb on - strand at 76.365 kb on - strand at 76.397 kb on - strand, within Ac3H11_186 at 76.510 kb on + strand, within Ac3H11_186 at 76.510 kb on + strand, within Ac3H11_186 at 76.510 kb on + strand, within Ac3H11_186 at 76.510 kb on + strand, within Ac3H11_186 at 76.511 kb on - strand, within Ac3H11_186 at 76.511 kb on - strand, within Ac3H11_186 at 76.511 kb on - strand, within Ac3H11_186 at 76.512 kb on - strand at 76.635 kb on + strand at 76.635 kb on + strand at 76.635 kb on + strand at 76.635 kb on + strand at 76.636 kb on - strand at 76.636 kb on - strand at 76.638 kb on + strand at 76.639 kb on - strand at 76.744 kb on + strand at 76.744 kb on + strand at 76.745 kb on - strand at 76.745 kb on - strand at 76.824 kb on - strand at 76.884 kb on + strand at 76.885 kb on - strand at 76.980 kb on - strand at 77.013 kb on + strand at 77.013 kb on + strand at 77.072 kb on - strand at 77.072 kb on - strand at 77.079 kb on + strand at 77.079 kb on + strand at 77.080 kb on - strand at 77.114 kb on + strand at 77.114 kb on + strand at 77.115 kb on - strand at 77.115 kb on - strand at 77.115 kb on - strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.185 kb on - strand at 77.185 kb on - strand at 77.185 kb on - strand at 77.190 kb on + strand at 77.190 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.313 kb on + strand at 77.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH9 remove 73,679 - Ac3H11_183 0.87 -1.0 73,679 - Ac3H11_183 0.87 -1.2 73,723 + Ac3H11_183 0.89 -0.1 73,723 + Ac3H11_183 0.89 +0.4 73,724 - Ac3H11_183 0.89 +0.2 73,927 + -1.9 73,927 + +0.1 73,927 + +0.6 73,927 + -0.3 73,927 + -0.5 73,928 - -0.0 73,928 - -0.2 73,983 + +0.5 74,047 + -0.2 74,048 - -0.6 74,048 - -0.4 74,101 + -0.8 74,155 + +0.6 74,155 + +0.9 74,155 + +0.6 74,155 + -0.4 74,156 - +0.6 74,156 - -0.6 74,156 - -0.5 74,156 - +0.7 74,156 - +0.1 74,156 - +0.2 74,156 - +1.6 74,156 - +1.1 74,156 - +0.4 74,434 + Ac3H11_184 0.63 +0.1 74,434 + Ac3H11_184 0.63 +1.3 74,776 + -0.6 74,776 + -0.0 74,776 + +0.8 74,776 + +0.0 74,777 - -1.0 74,777 - -0.1 74,777 - -2.3 74,777 - +0.4 74,777 - -0.5 74,777 - +0.1 74,777 - +0.2 74,777 - +0.4 74,777 - -0.2 75,013 + Ac3H11_185 0.20 +1.0 75,013 + Ac3H11_185 0.20 +0.1 75,113 - Ac3H11_185 0.26 -0.9 75,296 - Ac3H11_185 0.37 -1.9 75,307 + Ac3H11_185 0.37 -0.3 75,308 - Ac3H11_185 0.37 +0.2 75,319 + Ac3H11_185 0.38 -0.3 75,320 - Ac3H11_185 0.38 -1.3 75,379 + Ac3H11_185 0.42 +0.6 75,400 + Ac3H11_185 0.43 -0.5 75,400 + Ac3H11_185 0.43 -0.3 75,400 + Ac3H11_185 0.43 +0.7 75,400 + Ac3H11_185 0.43 -0.1 75,400 + Ac3H11_185 0.43 +1.4 75,400 + Ac3H11_185 0.43 -1.0 75,400 + Ac3H11_185 0.43 +0.3 75,400 + Ac3H11_185 0.43 -0.2 75,400 + Ac3H11_185 0.43 -0.3 75,400 + Ac3H11_185 0.43 +0.1 75,401 - Ac3H11_185 0.43 -0.3 75,401 - Ac3H11_185 0.43 -0.2 75,401 - Ac3H11_185 0.43 -0.5 75,401 - Ac3H11_185 0.43 +1.2 75,401 - Ac3H11_185 0.43 +0.3 75,401 - Ac3H11_185 0.43 -0.2 75,401 - Ac3H11_185 0.43 -0.4 75,401 - Ac3H11_185 0.43 -0.5 75,401 - Ac3H11_185 0.43 -0.4 75,401 - Ac3H11_185 0.43 -0.5 75,401 - Ac3H11_185 0.43 +0.2 75,541 + Ac3H11_185 0.51 -0.7 75,541 + Ac3H11_185 0.51 -1.0 75,541 + Ac3H11_185 0.51 +0.1 75,542 - Ac3H11_185 0.51 +0.2 75,542 - Ac3H11_185 0.51 +0.3 75,542 - Ac3H11_185 0.51 -0.5 75,542 - Ac3H11_185 0.51 -0.1 75,659 + Ac3H11_185 0.58 +0.2 75,715 + Ac3H11_185 0.61 -0.0 75,715 + Ac3H11_185 0.61 +0.1 75,715 + Ac3H11_185 0.61 -0.0 75,715 + Ac3H11_185 0.61 +0.7 75,715 + Ac3H11_185 0.61 +0.3 75,716 - Ac3H11_185 0.62 +0.4 75,716 - Ac3H11_185 0.62 +0.3 76,027 + Ac3H11_185 0.80 -0.1 76,027 + Ac3H11_185 0.80 +0.0 76,027 + Ac3H11_185 0.80 +0.7 76,027 + Ac3H11_185 0.80 -1.0 76,027 + Ac3H11_185 0.80 -0.9 76,028 - Ac3H11_185 0.80 +0.8 76,028 - Ac3H11_185 0.80 -0.4 76,028 - Ac3H11_185 0.80 +0.7 76,082 + Ac3H11_185 0.83 -0.7 76,082 + Ac3H11_185 0.83 -0.1 76,083 - Ac3H11_185 0.83 -1.3 76,083 - Ac3H11_185 0.83 +0.6 76,219 + +0.5 76,219 + +0.6 76,219 + +0.0 76,219 + -0.5 76,220 - -0.6 76,220 - -0.6 76,220 - -0.2 76,220 - +0.2 76,364 + +0.7 76,365 - -0.9 76,365 - -1.0 76,397 - Ac3H11_186 0.25 +0.5 76,510 + Ac3H11_186 0.89 +1.1 76,510 + Ac3H11_186 0.89 +0.2 76,510 + Ac3H11_186 0.89 +0.4 76,510 + Ac3H11_186 0.89 -0.1 76,511 - Ac3H11_186 0.89 -0.3 76,511 - Ac3H11_186 0.89 +0.8 76,511 - Ac3H11_186 0.89 -0.8 76,512 - +0.5 76,635 + +0.0 76,635 + +0.9 76,635 + -0.4 76,635 + -0.9 76,636 - +1.0 76,636 - +0.0 76,638 + -0.4 76,639 - +0.4 76,744 + -0.3 76,744 + -0.1 76,745 - +2.1 76,745 - +0.1 76,824 - +1.0 76,884 + -0.4 76,885 - -0.3 76,980 - +0.1 77,013 + -0.9 77,013 + +0.9 77,072 - -0.1 77,072 - -0.8 77,079 + +1.5 77,079 + +0.1 77,080 - -0.4 77,114 + +1.0 77,114 + -0.2 77,115 - -1.2 77,115 - -0.0 77,115 - -0.2 77,182 + -1.5 77,182 + -0.1 77,182 + +0.3 77,182 + +0.2 77,182 + +0.2 77,182 + +0.0 77,182 + -0.1 77,182 + +0.7 77,183 - +0.1 77,183 - +0.5 77,183 - +1.3 77,183 - -0.0 77,183 - +0.9 77,183 - +0.2 77,184 + -0.6 77,184 + -0.0 77,184 + -0.3 77,184 + -0.2 77,184 + -0.5 77,184 + -0.3 77,184 + -0.1 77,184 + +0.9 77,185 - +0.1 77,185 - +0.5 77,185 - +0.4 77,190 + +0.8 77,190 + -0.6 77,279 + -0.8 77,279 + +1.2 77,279 + -1.5 77,279 + +0.2 77,279 + +0.6 77,279 + +1.8 77,279 + +1.0 77,279 + +0.1 77,279 + +0.3 77,279 + -0.1 77,280 - -1.0 77,280 - -0.5 77,280 - +0.4 77,280 - +0.4 77,280 - -0.3 77,280 - +1.6 77,280 - -0.7 77,280 - -0.3 77,280 - -0.1 77,280 - -0.1 77,280 - +1.6 77,280 - +0.0 77,280 - +0.3 77,280 - -1.1 77,280 - -0.4 77,280 - -0.3 77,280 - -0.7 77,280 - -0.4 77,313 + +1.0 77,314 - -0.1
Or see this region's nucleotide sequence