Experiment: L-Malic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5510 and Pf1N1B4_5511 are separated by 910 nucleotides Pf1N1B4_5511 and Pf1N1B4_5512 are separated by 342 nucleotides Pf1N1B4_5512 and Pf1N1B4_5513 are separated by 75 nucleotides Pf1N1B4_5513 and Pf1N1B4_5514 are separated by 69 nucleotides
Pf1N1B4_5510 - hypothetical protein, at 259,450 to 259,563
_5510
Pf1N1B4_5511 - hypothetical protein, at 260,474 to 260,755
_5511
Pf1N1B4_5512 - hypothetical protein, at 261,098 to 261,244
_5512
Pf1N1B4_5513 - hypothetical protein, at 261,320 to 261,517
_5513
Pf1N1B4_5514 - RecA protein, at 261,587 to 262,636
_5514
Position (kb) 260
261 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 259.514 kb on + strand, within Pf1N1B4_5510 at 259.571 kb on + strand at 259.571 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.575 kb on + strand at 259.576 kb on - strand at 259.576 kb on - strand at 259.617 kb on + strand at 259.707 kb on - strand at 259.729 kb on + strand at 259.730 kb on - strand at 259.730 kb on - strand at 259.730 kb on - strand at 259.730 kb on - strand at 259.733 kb on + strand at 259.733 kb on + strand at 259.733 kb on + strand at 259.734 kb on - strand at 259.734 kb on - strand at 259.734 kb on - strand at 259.734 kb on - strand at 259.758 kb on + strand at 259.758 kb on + strand at 259.758 kb on + strand at 259.797 kb on + strand at 259.855 kb on - strand at 259.919 kb on - strand at 259.960 kb on + strand at 259.960 kb on + strand at 259.961 kb on - strand at 259.984 kb on + strand at 259.984 kb on + strand at 259.985 kb on - strand at 260.018 kb on - strand at 260.099 kb on + strand at 260.100 kb on - strand at 260.100 kb on - strand at 260.165 kb on + strand at 260.166 kb on - strand at 260.166 kb on - strand at 260.252 kb on + strand at 260.253 kb on - strand at 260.383 kb on + strand at 260.384 kb on - strand at 260.408 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.476 kb on - strand at 260.476 kb on - strand at 260.476 kb on - strand at 260.499 kb on + strand at 260.499 kb on + strand at 260.500 kb on - strand at 260.510 kb on + strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.575 kb on - strand, within Pf1N1B4_5511 at 260.639 kb on + strand, within Pf1N1B4_5511 at 260.639 kb on + strand, within Pf1N1B4_5511 at 260.640 kb on - strand, within Pf1N1B4_5511 at 260.640 kb on - strand, within Pf1N1B4_5511 at 260.691 kb on - strand, within Pf1N1B4_5511 at 260.786 kb on + strand at 260.786 kb on + strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.790 kb on + strand at 260.920 kb on + strand at 260.920 kb on + strand at 261.018 kb on + strand at 261.018 kb on + strand at 261.164 kb on - strand, within Pf1N1B4_5512 at 261.164 kb on - strand, within Pf1N1B4_5512 at 261.166 kb on - strand, within Pf1N1B4_5512 at 261.222 kb on - strand, within Pf1N1B4_5512 at 261.260 kb on + strand at 261.260 kb on + strand at 261.261 kb on - strand at 261.261 kb on - strand at 261.265 kb on + strand at 261.265 kb on + strand at 261.266 kb on - strand at 261.266 kb on - strand at 261.287 kb on - strand at 261.287 kb on - strand at 261.296 kb on - strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.321 kb on + strand at 261.321 kb on + strand at 261.321 kb on + strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.458 kb on + strand, within Pf1N1B4_5513 at 261.458 kb on + strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.468 kb on + strand, within Pf1N1B4_5513 at 261.534 kb on + strand at 261.535 kb on - strand at 261.550 kb on - strand at 261.555 kb on - strand at 261.668 kb on - strand at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) remove 259,514 + Pf1N1B4_5510 0.56 +1.3 259,571 + +0.0 259,571 + +0.6 259,575 + +0.6 259,575 + -0.3 259,575 + -1.0 259,575 + -0.4 259,575 + +1.0 259,575 + -1.4 259,576 - -1.4 259,576 - -0.4 259,617 + -1.0 259,707 - +1.0 259,729 + -1.0 259,730 - -0.2 259,730 - +1.0 259,730 - -1.6 259,730 - -0.6 259,733 + -1.0 259,733 + -0.4 259,733 + -2.0 259,734 - -0.3 259,734 - -0.4 259,734 - -2.0 259,734 - -0.3 259,758 + -1.0 259,758 + -1.0 259,758 + +0.4 259,797 + -1.6 259,855 - -1.3 259,919 - +0.7 259,960 + +0.4 259,960 + +0.0 259,961 - +0.4 259,984 + -0.6 259,984 + +2.0 259,985 - -2.6 260,018 - -1.3 260,099 + +0.6 260,100 - +0.0 260,100 - -0.3 260,165 + -2.3 260,166 - -0.2 260,166 - -1.2 260,252 + +0.7 260,253 - +2.0 260,383 + -1.0 260,384 - -0.6 260,408 + -0.7 260,413 + -1.6 260,413 + +0.0 260,413 + +0.0 260,413 + +2.0 260,414 - -0.9 260,414 - +1.0 260,414 - -0.6 260,414 - -1.3 260,414 - +0.0 260,414 - +1.8 260,414 - -0.6 260,414 - -0.6 260,476 - -1.6 260,476 - -0.7 260,476 - +0.0 260,499 + -0.6 260,499 + -1.7 260,500 - -0.2 260,510 + Pf1N1B4_5511 0.13 -1.0 260,511 - Pf1N1B4_5511 0.13 -0.6 260,511 - Pf1N1B4_5511 0.13 +1.6 260,511 - Pf1N1B4_5511 0.13 -0.3 260,511 - Pf1N1B4_5511 0.13 -0.3 260,575 - Pf1N1B4_5511 0.36 -1.7 260,639 + Pf1N1B4_5511 0.59 +0.5 260,639 + Pf1N1B4_5511 0.59 -0.9 260,640 - Pf1N1B4_5511 0.59 -1.0 260,640 - Pf1N1B4_5511 0.59 -1.6 260,691 - Pf1N1B4_5511 0.77 +0.0 260,786 + +0.2 260,786 + -1.8 260,787 - +2.2 260,787 - -0.7 260,787 - -0.1 260,787 - -0.3 260,790 + +0.0 260,920 + +1.2 260,920 + +1.0 261,018 + +1.0 261,018 + +0.3 261,164 - Pf1N1B4_5512 0.45 -0.2 261,164 - Pf1N1B4_5512 0.45 -0.7 261,166 - Pf1N1B4_5512 0.46 +2.8 261,222 - Pf1N1B4_5512 0.84 +2.3 261,260 + -0.6 261,260 + -1.6 261,261 - -1.6 261,261 - -1.6 261,265 + +1.6 261,265 + +0.0 261,266 - -1.3 261,266 - -1.6 261,287 - +0.4 261,287 - -2.3 261,296 - -0.6 261,316 + -1.6 261,316 + -0.3 261,317 - +0.0 261,317 - -1.0 261,321 + -1.6 261,321 + +0.6 261,321 + -2.0 261,322 - -1.0 261,322 - +0.4 261,322 - +0.8 261,322 - -1.3 261,458 + Pf1N1B4_5513 0.70 -0.7 261,458 + Pf1N1B4_5513 0.70 -0.5 261,459 - Pf1N1B4_5513 0.70 +0.0 261,459 - Pf1N1B4_5513 0.70 +0.0 261,459 - Pf1N1B4_5513 0.70 -1.0 261,468 + Pf1N1B4_5513 0.75 +0.5 261,534 + +0.6 261,535 - -1.4 261,550 - -1.6 261,555 - -2.3 261,668 - +0.0 261,712 - Pf1N1B4_5514 0.12 +0.6 261,712 - Pf1N1B4_5514 0.12 -2.6 261,712 - Pf1N1B4_5514 0.12 +1.0 261,712 - Pf1N1B4_5514 0.12 -0.6 261,712 - Pf1N1B4_5514 0.12 +0.7 261,712 - Pf1N1B4_5514 0.12 +0.4 261,712 - Pf1N1B4_5514 0.12 -0.9
Or see this region's nucleotide sequence