Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1925 and Ac3H11_1926 are separated by 154 nucleotides Ac3H11_1926 and Ac3H11_1927 overlap by 4 nucleotides
Ac3H11_1925 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 135,351 to 136,526
_1925
Ac3H11_1926 - Isohexenylglutaconyl-CoA hydratase, at 136,681 to 137,529
_1926
Ac3H11_1927 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 137,526 to 139,538
_1927
Position (kb) 136
137
138 Strain fitness (log2 ratio) -1
0
1 at 135.792 kb on + strand, within Ac3H11_1925 at 135.793 kb on - strand, within Ac3H11_1925 at 135.793 kb on - strand, within Ac3H11_1925 at 135.793 kb on - strand, within Ac3H11_1925 at 136.008 kb on + strand, within Ac3H11_1925 at 136.009 kb on - strand, within Ac3H11_1925 at 136.009 kb on - strand, within Ac3H11_1925 at 136.009 kb on - strand, within Ac3H11_1925 at 136.071 kb on + strand, within Ac3H11_1925 at 136.071 kb on + strand, within Ac3H11_1925 at 136.071 kb on + strand, within Ac3H11_1925 at 136.270 kb on - strand, within Ac3H11_1925 at 136.287 kb on + strand, within Ac3H11_1925 at 136.287 kb on + strand, within Ac3H11_1925 at 136.287 kb on + strand, within Ac3H11_1925 at 136.287 kb on + strand, within Ac3H11_1925 at 136.287 kb on + strand, within Ac3H11_1925 at 136.287 kb on + strand, within Ac3H11_1925 at 136.287 kb on + strand, within Ac3H11_1925 at 136.288 kb on - strand, within Ac3H11_1925 at 136.288 kb on - strand, within Ac3H11_1925 at 136.288 kb on - strand, within Ac3H11_1925 at 136.288 kb on - strand, within Ac3H11_1925 at 136.288 kb on - strand, within Ac3H11_1925 at 136.399 kb on - strand, within Ac3H11_1925 at 136.429 kb on - strand at 136.429 kb on - strand at 136.429 kb on - strand at 136.522 kb on + strand at 136.551 kb on + strand at 136.569 kb on + strand at 136.570 kb on - strand at 137.117 kb on + strand, within Ac3H11_1926 at 137.118 kb on - strand, within Ac3H11_1926 at 137.118 kb on - strand, within Ac3H11_1926 at 137.319 kb on - strand, within Ac3H11_1926 at 137.400 kb on + strand, within Ac3H11_1926 at 137.400 kb on + strand, within Ac3H11_1926 at 137.401 kb on - strand, within Ac3H11_1926 at 137.542 kb on + strand at 137.543 kb on - strand at 137.617 kb on - strand at 137.700 kb on + strand at 137.700 kb on + strand at 137.700 kb on + strand at 137.701 kb on - strand at 137.767 kb on - strand, within Ac3H11_1927 at 138.043 kb on + strand, within Ac3H11_1927 at 138.044 kb on - strand, within Ac3H11_1927 at 138.324 kb on + strand, within Ac3H11_1927 at 138.324 kb on + strand, within Ac3H11_1927 at 138.324 kb on + strand, within Ac3H11_1927 at 138.325 kb on - strand, within Ac3H11_1927 at 138.325 kb on - strand, within Ac3H11_1927 at 138.325 kb on - strand, within Ac3H11_1927
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 135,792 + Ac3H11_1925 0.38 +1.6 135,793 - Ac3H11_1925 0.38 +0.8 135,793 - Ac3H11_1925 0.38 -0.8 135,793 - Ac3H11_1925 0.38 +0.9 136,008 + Ac3H11_1925 0.56 +0.3 136,009 - Ac3H11_1925 0.56 -0.1 136,009 - Ac3H11_1925 0.56 -0.2 136,009 - Ac3H11_1925 0.56 +0.2 136,071 + Ac3H11_1925 0.61 +0.8 136,071 + Ac3H11_1925 0.61 +0.4 136,071 + Ac3H11_1925 0.61 +0.0 136,270 - Ac3H11_1925 0.78 +0.1 136,287 + Ac3H11_1925 0.80 +0.3 136,287 + Ac3H11_1925 0.80 -0.3 136,287 + Ac3H11_1925 0.80 +0.9 136,287 + Ac3H11_1925 0.80 -0.3 136,287 + Ac3H11_1925 0.80 +0.5 136,287 + Ac3H11_1925 0.80 -0.1 136,287 + Ac3H11_1925 0.80 +0.3 136,288 - Ac3H11_1925 0.80 -0.3 136,288 - Ac3H11_1925 0.80 +0.3 136,288 - Ac3H11_1925 0.80 +0.7 136,288 - Ac3H11_1925 0.80 +0.1 136,288 - Ac3H11_1925 0.80 -0.2 136,399 - Ac3H11_1925 0.89 +0.5 136,429 - +0.5 136,429 - +0.6 136,429 - +0.5 136,522 + +0.1 136,551 + +0.6 136,569 + -0.5 136,570 - -0.3 137,117 + Ac3H11_1926 0.51 -1.2 137,118 - Ac3H11_1926 0.51 -0.3 137,118 - Ac3H11_1926 0.51 -0.1 137,319 - Ac3H11_1926 0.75 -0.0 137,400 + Ac3H11_1926 0.85 -0.1 137,400 + Ac3H11_1926 0.85 -0.4 137,401 - Ac3H11_1926 0.85 -0.6 137,542 + -0.7 137,543 - +0.9 137,617 - +0.4 137,700 + +0.2 137,700 + -0.4 137,700 + +0.8 137,701 - +0.1 137,767 - Ac3H11_1927 0.12 -0.8 138,043 + Ac3H11_1927 0.26 +0.5 138,044 - Ac3H11_1927 0.26 -0.2 138,324 + Ac3H11_1927 0.40 -0.1 138,324 + Ac3H11_1927 0.40 -0.1 138,324 + Ac3H11_1927 0.40 -0.4 138,325 - Ac3H11_1927 0.40 -0.2 138,325 - Ac3H11_1927 0.40 +0.0 138,325 - Ac3H11_1927 0.40 -0.1
Or see this region's nucleotide sequence