Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30000 and RR42_RS30005 are separated by 586 nucleotides RR42_RS30005 and RR42_RS30010 are separated by 165 nucleotides
RR42_RS30000: RR42_RS30000 - LacI family transcriptional regulator, at 2,095,878 to 2,096,900
_RS30000
RR42_RS30005: RR42_RS30005 - LysR family transcriptional regulator, at 2,097,487 to 2,098,401
_RS30005
RR42_RS30010: RR42_RS30010 - sterol desaturase, at 2,098,567 to 2,099,712
_RS30010
Position (kb)
2097
2098
2099 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2096.535 kb on + strand, within RR42_RS30000 at 2096.535 kb on + strand, within RR42_RS30000 at 2096.624 kb on - strand, within RR42_RS30000 at 2096.624 kb on - strand, within RR42_RS30000 at 2096.740 kb on + strand, within RR42_RS30000 at 2096.792 kb on + strand, within RR42_RS30000 at 2096.792 kb on + strand, within RR42_RS30000 at 2096.793 kb on - strand, within RR42_RS30000 at 2096.864 kb on - strand at 2096.866 kb on + strand at 2096.867 kb on - strand at 2096.879 kb on + strand at 2096.879 kb on + strand at 2096.945 kb on + strand at 2096.945 kb on + strand at 2096.945 kb on + strand at 2096.946 kb on - strand at 2096.946 kb on - strand at 2096.946 kb on - strand at 2096.974 kb on + strand at 2097.114 kb on + strand at 2097.203 kb on - strand at 2097.203 kb on - strand at 2097.216 kb on + strand at 2097.216 kb on + strand at 2097.216 kb on + strand at 2097.217 kb on - strand at 2097.217 kb on - strand at 2097.217 kb on - strand at 2097.217 kb on - strand at 2097.217 kb on - strand at 2097.217 kb on - strand at 2097.253 kb on + strand at 2097.304 kb on + strand at 2097.304 kb on + strand at 2097.304 kb on + strand at 2097.315 kb on + strand at 2097.316 kb on - strand at 2097.316 kb on - strand at 2097.319 kb on + strand at 2097.319 kb on + strand at 2097.319 kb on + strand at 2097.319 kb on + strand at 2097.319 kb on + strand at 2097.319 kb on + strand at 2097.320 kb on - strand at 2097.373 kb on + strand at 2097.571 kb on + strand at 2097.571 kb on + strand at 2097.572 kb on - strand at 2097.572 kb on - strand at 2097.629 kb on + strand, within RR42_RS30005 at 2097.629 kb on + strand, within RR42_RS30005 at 2097.629 kb on + strand, within RR42_RS30005 at 2097.629 kb on + strand, within RR42_RS30005 at 2097.629 kb on + strand, within RR42_RS30005 at 2097.629 kb on + strand, within RR42_RS30005 at 2097.629 kb on + strand, within RR42_RS30005 at 2097.630 kb on - strand, within RR42_RS30005 at 2097.630 kb on - strand, within RR42_RS30005 at 2097.630 kb on - strand, within RR42_RS30005 at 2097.630 kb on - strand, within RR42_RS30005 at 2097.670 kb on - strand, within RR42_RS30005 at 2097.713 kb on + strand, within RR42_RS30005 at 2097.714 kb on - strand, within RR42_RS30005 at 2097.827 kb on + strand, within RR42_RS30005 at 2097.827 kb on + strand, within RR42_RS30005 at 2097.827 kb on + strand, within RR42_RS30005 at 2097.935 kb on + strand, within RR42_RS30005 at 2097.936 kb on - strand, within RR42_RS30005 at 2097.936 kb on - strand, within RR42_RS30005 at 2097.941 kb on + strand, within RR42_RS30005 at 2097.941 kb on + strand, within RR42_RS30005 at 2097.941 kb on + strand, within RR42_RS30005 at 2097.942 kb on - strand, within RR42_RS30005 at 2097.942 kb on - strand, within RR42_RS30005 at 2098.009 kb on + strand, within RR42_RS30005 at 2098.010 kb on - strand, within RR42_RS30005 at 2098.072 kb on + strand, within RR42_RS30005 at 2098.073 kb on - strand, within RR42_RS30005 at 2098.082 kb on + strand, within RR42_RS30005 at 2098.082 kb on + strand, within RR42_RS30005 at 2098.082 kb on + strand, within RR42_RS30005 at 2098.082 kb on + strand, within RR42_RS30005 at 2098.083 kb on - strand, within RR42_RS30005 at 2098.451 kb on + strand at 2098.452 kb on - strand at 2098.452 kb on - strand at 2098.452 kb on - strand at 2098.452 kb on - strand at 2098.456 kb on + strand at 2098.456 kb on + strand at 2098.456 kb on + strand at 2098.456 kb on + strand at 2098.456 kb on + strand at 2098.456 kb on + strand at 2098.456 kb on + strand at 2098.457 kb on - strand at 2098.457 kb on - strand at 2098.473 kb on + strand at 2098.473 kb on + strand at 2098.526 kb on + strand at 2098.526 kb on + strand at 2098.622 kb on - strand at 2098.622 kb on - strand at 2098.624 kb on + strand at 2098.624 kb on + strand at 2098.624 kb on + strand at 2098.624 kb on + strand at 2098.624 kb on + strand at 2098.624 kb on + strand at 2098.624 kb on + strand at 2098.624 kb on + strand at 2098.625 kb on - strand at 2098.625 kb on - strand at 2098.625 kb on - strand at 2098.625 kb on - strand at 2098.625 kb on - strand at 2098.625 kb on - strand at 2098.742 kb on + strand, within RR42_RS30010 at 2098.742 kb on + strand, within RR42_RS30010 at 2098.743 kb on - strand, within RR42_RS30010 at 2098.765 kb on + strand, within RR42_RS30010 at 2098.819 kb on + strand, within RR42_RS30010 at 2098.845 kb on + strand, within RR42_RS30010 at 2098.873 kb on + strand, within RR42_RS30010 at 2098.930 kb on + strand, within RR42_RS30010 at 2098.931 kb on - strand, within RR42_RS30010 at 2099.009 kb on + strand, within RR42_RS30010 at 2099.020 kb on + strand, within RR42_RS30010 at 2099.029 kb on + strand, within RR42_RS30010 at 2099.029 kb on + strand, within RR42_RS30010 at 2099.188 kb on + strand, within RR42_RS30010 at 2099.188 kb on + strand, within RR42_RS30010 at 2099.188 kb on + strand, within RR42_RS30010 at 2099.188 kb on + strand, within RR42_RS30010 at 2099.189 kb on - strand, within RR42_RS30010 at 2099.189 kb on - strand, within RR42_RS30010 at 2099.265 kb on + strand, within RR42_RS30010 at 2099.296 kb on + strand, within RR42_RS30010 at 2099.296 kb on + strand, within RR42_RS30010 at 2099.296 kb on + strand, within RR42_RS30010 at 2099.296 kb on + strand, within RR42_RS30010 at 2099.304 kb on + strand, within RR42_RS30010 at 2099.335 kb on + strand, within RR42_RS30010 at 2099.335 kb on + strand, within RR42_RS30010 at 2099.335 kb on + strand, within RR42_RS30010 at 2099.336 kb on - strand, within RR42_RS30010 at 2099.336 kb on - strand, within RR42_RS30010 at 2099.336 kb on - strand, within RR42_RS30010 at 2099.342 kb on + strand, within RR42_RS30010 at 2099.342 kb on + strand, within RR42_RS30010 at 2099.342 kb on + strand, within RR42_RS30010 at 2099.343 kb on - strand, within RR42_RS30010 at 2099.392 kb on + strand, within RR42_RS30010 at 2099.393 kb on - strand, within RR42_RS30010 at 2099.393 kb on - strand, within RR42_RS30010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 2,096,535 + RR42_RS30000 0.64 -0.2 2,096,535 + RR42_RS30000 0.64 +0.8 2,096,624 - RR42_RS30000 0.73 +0.4 2,096,624 - RR42_RS30000 0.73 +0.0 2,096,740 + RR42_RS30000 0.84 -1.2 2,096,792 + RR42_RS30000 0.89 -1.0 2,096,792 + RR42_RS30000 0.89 +0.2 2,096,793 - RR42_RS30000 0.89 -0.2 2,096,864 - +0.0 2,096,866 + -0.6 2,096,867 - +0.7 2,096,879 + +0.4 2,096,879 + -1.0 2,096,945 + +0.0 2,096,945 + -0.5 2,096,945 + -0.5 2,096,946 - -0.0 2,096,946 - +0.4 2,096,946 - +0.1 2,096,974 + -0.1 2,097,114 + -0.4 2,097,203 - -0.5 2,097,203 - -0.2 2,097,216 + -0.1 2,097,216 + -0.6 2,097,216 + -0.9 2,097,217 - -0.3 2,097,217 - -0.3 2,097,217 - -1.2 2,097,217 - -1.6 2,097,217 - -0.6 2,097,217 - -1.4 2,097,253 + +0.4 2,097,304 + -0.3 2,097,304 + +0.3 2,097,304 + -0.2 2,097,315 + -0.2 2,097,316 - -0.3 2,097,316 - -0.1 2,097,319 + -0.3 2,097,319 + -0.1 2,097,319 + +1.4 2,097,319 + -0.1 2,097,319 + -0.8 2,097,319 + +0.5 2,097,320 - +0.5 2,097,373 + +0.1 2,097,571 + +1.1 2,097,571 + -0.1 2,097,572 - -0.5 2,097,572 - +0.3 2,097,629 + RR42_RS30005 0.16 -0.6 2,097,629 + RR42_RS30005 0.16 +0.0 2,097,629 + RR42_RS30005 0.16 +0.1 2,097,629 + RR42_RS30005 0.16 +0.3 2,097,629 + RR42_RS30005 0.16 -0.1 2,097,629 + RR42_RS30005 0.16 +0.4 2,097,629 + RR42_RS30005 0.16 -0.6 2,097,630 - RR42_RS30005 0.16 +0.8 2,097,630 - RR42_RS30005 0.16 -0.7 2,097,630 - RR42_RS30005 0.16 -0.7 2,097,630 - RR42_RS30005 0.16 -0.9 2,097,670 - RR42_RS30005 0.20 +0.3 2,097,713 + RR42_RS30005 0.25 -1.5 2,097,714 - RR42_RS30005 0.25 +0.6 2,097,827 + RR42_RS30005 0.37 -0.7 2,097,827 + RR42_RS30005 0.37 -0.2 2,097,827 + RR42_RS30005 0.37 -0.8 2,097,935 + RR42_RS30005 0.49 -1.2 2,097,936 - RR42_RS30005 0.49 -0.3 2,097,936 - RR42_RS30005 0.49 +1.0 2,097,941 + RR42_RS30005 0.50 +0.2 2,097,941 + RR42_RS30005 0.50 -0.1 2,097,941 + RR42_RS30005 0.50 -0.9 2,097,942 - RR42_RS30005 0.50 -0.1 2,097,942 - RR42_RS30005 0.50 -0.1 2,098,009 + RR42_RS30005 0.57 -0.9 2,098,010 - RR42_RS30005 0.57 -0.1 2,098,072 + RR42_RS30005 0.64 -0.9 2,098,073 - RR42_RS30005 0.64 -0.2 2,098,082 + RR42_RS30005 0.65 -0.7 2,098,082 + RR42_RS30005 0.65 -0.1 2,098,082 + RR42_RS30005 0.65 +0.6 2,098,082 + RR42_RS30005 0.65 -0.2 2,098,083 - RR42_RS30005 0.65 -0.7 2,098,451 + -0.6 2,098,452 - -0.2 2,098,452 - -0.7 2,098,452 - -1.2 2,098,452 - +0.5 2,098,456 + -0.8 2,098,456 + -0.1 2,098,456 + -0.5 2,098,456 + -0.1 2,098,456 + +0.4 2,098,456 + +0.4 2,098,456 + -0.6 2,098,457 - +1.0 2,098,457 - +1.1 2,098,473 + +0.5 2,098,473 + +0.6 2,098,526 + +0.7 2,098,526 + -0.5 2,098,622 - -1.9 2,098,622 - +1.0 2,098,624 + +0.4 2,098,624 + -0.3 2,098,624 + -0.2 2,098,624 + +1.1 2,098,624 + -1.0 2,098,624 + -0.2 2,098,624 + +0.1 2,098,624 + -0.6 2,098,625 - +0.1 2,098,625 - -1.1 2,098,625 - -0.2 2,098,625 - -0.7 2,098,625 - +0.4 2,098,625 - -0.1 2,098,742 + RR42_RS30010 0.15 -0.9 2,098,742 + RR42_RS30010 0.15 +0.1 2,098,743 - RR42_RS30010 0.15 -0.2 2,098,765 + RR42_RS30010 0.17 +0.8 2,098,819 + RR42_RS30010 0.22 +1.6 2,098,845 + RR42_RS30010 0.24 +0.5 2,098,873 + RR42_RS30010 0.27 -0.5 2,098,930 + RR42_RS30010 0.32 +0.3 2,098,931 - RR42_RS30010 0.32 -0.8 2,099,009 + RR42_RS30010 0.39 +0.9 2,099,020 + RR42_RS30010 0.40 -0.8 2,099,029 + RR42_RS30010 0.40 +0.2 2,099,029 + RR42_RS30010 0.40 +1.4 2,099,188 + RR42_RS30010 0.54 +1.4 2,099,188 + RR42_RS30010 0.54 -0.7 2,099,188 + RR42_RS30010 0.54 -1.2 2,099,188 + RR42_RS30010 0.54 +3.7 2,099,189 - RR42_RS30010 0.54 -0.5 2,099,189 - RR42_RS30010 0.54 -2.3 2,099,265 + RR42_RS30010 0.61 +0.6 2,099,296 + RR42_RS30010 0.64 +0.5 2,099,296 + RR42_RS30010 0.64 -0.7 2,099,296 + RR42_RS30010 0.64 +0.8 2,099,296 + RR42_RS30010 0.64 -0.7 2,099,304 + RR42_RS30010 0.64 +0.0 2,099,335 + RR42_RS30010 0.67 +1.7 2,099,335 + RR42_RS30010 0.67 +0.1 2,099,335 + RR42_RS30010 0.67 +0.1 2,099,336 - RR42_RS30010 0.67 +0.3 2,099,336 - RR42_RS30010 0.67 +0.7 2,099,336 - RR42_RS30010 0.67 +0.2 2,099,342 + RR42_RS30010 0.68 -0.0 2,099,342 + RR42_RS30010 0.68 +0.3 2,099,342 + RR42_RS30010 0.68 +0.8 2,099,343 - RR42_RS30010 0.68 -0.1 2,099,392 + RR42_RS30010 0.72 +0.6 2,099,393 - RR42_RS30010 0.72 +0.7 2,099,393 - RR42_RS30010 0.72 -2.3
Or see this region's nucleotide sequence