Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29645 and RR42_RS29650 are separated by 276 nucleotides RR42_RS29650 and RR42_RS29655 are separated by 3 nucleotides RR42_RS29655 and RR42_RS29660 are separated by 59 nucleotides
RR42_RS29645: RR42_RS29645 - ABC transporter substrate-binding protein, at 2,019,268 to 2,019,867
_RS29645
RR42_RS29650: RR42_RS29650 - histidine ABC transporter permease, at 2,020,144 to 2,020,833
_RS29650
RR42_RS29655: RR42_RS29655 - amino acid ABC transporter permease, at 2,020,837 to 2,021,550
_RS29655
RR42_RS29660: RR42_RS29660 - amino acid transporter, at 2,021,610 to 2,022,404
_RS29660
Position (kb)
2020
2021
2022 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2019.838 kb on + strand at 2019.838 kb on + strand at 2019.856 kb on + strand at 2019.857 kb on - strand at 2019.857 kb on - strand at 2019.857 kb on - strand at 2019.857 kb on - strand at 2019.862 kb on - strand at 2019.862 kb on - strand at 2019.938 kb on + strand at 2019.939 kb on - strand at 2019.939 kb on - strand at 2019.939 kb on - strand at 2019.973 kb on + strand at 2019.974 kb on - strand at 2019.974 kb on - strand at 2019.977 kb on + strand at 2019.977 kb on + strand at 2019.977 kb on + strand at 2019.978 kb on - strand at 2019.978 kb on - strand at 2019.978 kb on - strand at 2019.978 kb on - strand at 2019.978 kb on - strand at 2019.978 kb on - strand at 2019.985 kb on - strand at 2020.000 kb on + strand at 2020.014 kb on + strand at 2020.015 kb on - strand at 2020.015 kb on - strand at 2020.103 kb on + strand at 2020.104 kb on - strand at 2020.104 kb on - strand at 2020.154 kb on - strand at 2020.154 kb on - strand at 2020.172 kb on + strand at 2020.172 kb on + strand at 2020.188 kb on + strand at 2020.194 kb on + strand at 2020.195 kb on - strand at 2020.293 kb on + strand, within RR42_RS29650 at 2020.294 kb on - strand, within RR42_RS29650 at 2020.294 kb on - strand, within RR42_RS29650 at 2020.294 kb on - strand, within RR42_RS29650 at 2020.294 kb on - strand, within RR42_RS29650 at 2020.294 kb on - strand, within RR42_RS29650 at 2020.300 kb on + strand, within RR42_RS29650 at 2020.301 kb on - strand, within RR42_RS29650 at 2020.301 kb on - strand, within RR42_RS29650 at 2020.311 kb on + strand, within RR42_RS29650 at 2020.337 kb on + strand, within RR42_RS29650 at 2020.337 kb on + strand, within RR42_RS29650 at 2020.337 kb on + strand, within RR42_RS29650 at 2020.439 kb on + strand, within RR42_RS29650 at 2020.465 kb on + strand, within RR42_RS29650 at 2020.466 kb on - strand, within RR42_RS29650 at 2020.466 kb on - strand, within RR42_RS29650 at 2020.470 kb on + strand, within RR42_RS29650 at 2020.470 kb on + strand, within RR42_RS29650 at 2020.470 kb on + strand, within RR42_RS29650 at 2020.534 kb on + strand, within RR42_RS29650 at 2020.535 kb on - strand, within RR42_RS29650 at 2020.556 kb on + strand, within RR42_RS29650 at 2020.557 kb on - strand, within RR42_RS29650 at 2020.748 kb on - strand, within RR42_RS29650 at 2020.748 kb on - strand, within RR42_RS29650 at 2020.756 kb on - strand, within RR42_RS29650 at 2020.801 kb on + strand at 2020.801 kb on + strand at 2020.802 kb on - strand at 2020.802 kb on - strand at 2020.831 kb on + strand at 2020.832 kb on - strand at 2020.845 kb on + strand at 2020.877 kb on - strand at 2020.951 kb on - strand, within RR42_RS29655 at 2020.980 kb on + strand, within RR42_RS29655 at 2020.980 kb on + strand, within RR42_RS29655 at 2020.981 kb on - strand, within RR42_RS29655 at 2021.021 kb on - strand, within RR42_RS29655 at 2021.021 kb on - strand, within RR42_RS29655 at 2021.026 kb on + strand, within RR42_RS29655 at 2021.026 kb on + strand, within RR42_RS29655 at 2021.026 kb on + strand, within RR42_RS29655 at 2021.026 kb on + strand, within RR42_RS29655 at 2021.027 kb on - strand, within RR42_RS29655 at 2021.027 kb on - strand, within RR42_RS29655 at 2021.027 kb on - strand, within RR42_RS29655 at 2021.031 kb on - strand, within RR42_RS29655 at 2021.031 kb on - strand, within RR42_RS29655 at 2021.072 kb on - strand, within RR42_RS29655 at 2021.072 kb on - strand, within RR42_RS29655 at 2021.140 kb on + strand, within RR42_RS29655 at 2021.143 kb on + strand, within RR42_RS29655 at 2021.144 kb on - strand, within RR42_RS29655 at 2021.144 kb on - strand, within RR42_RS29655 at 2021.182 kb on + strand, within RR42_RS29655 at 2021.276 kb on - strand, within RR42_RS29655 at 2021.323 kb on + strand, within RR42_RS29655 at 2021.324 kb on - strand, within RR42_RS29655 at 2021.355 kb on + strand, within RR42_RS29655 at 2021.355 kb on + strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.356 kb on - strand, within RR42_RS29655 at 2021.425 kb on + strand, within RR42_RS29655 at 2021.425 kb on + strand, within RR42_RS29655 at 2021.425 kb on + strand, within RR42_RS29655 at 2021.425 kb on + strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.426 kb on - strand, within RR42_RS29655 at 2021.464 kb on + strand, within RR42_RS29655 at 2021.465 kb on - strand, within RR42_RS29655 at 2021.528 kb on - strand at 2021.528 kb on - strand at 2021.528 kb on - strand at 2021.548 kb on + strand at 2021.548 kb on + strand at 2021.549 kb on - strand at 2021.549 kb on - strand at 2021.615 kb on - strand at 2021.615 kb on - strand at 2021.689 kb on + strand at 2021.689 kb on + strand at 2021.689 kb on + strand at 2021.726 kb on + strand, within RR42_RS29660 at 2021.732 kb on + strand, within RR42_RS29660 at 2021.732 kb on + strand, within RR42_RS29660 at 2021.732 kb on + strand, within RR42_RS29660 at 2022.033 kb on + strand, within RR42_RS29660 at 2022.033 kb on + strand, within RR42_RS29660 at 2022.034 kb on - strand, within RR42_RS29660 at 2022.075 kb on + strand, within RR42_RS29660 at 2022.076 kb on - strand, within RR42_RS29660 at 2022.076 kb on - strand, within RR42_RS29660 at 2022.076 kb on - strand, within RR42_RS29660 at 2022.167 kb on + strand, within RR42_RS29660 at 2022.167 kb on + strand, within RR42_RS29660 at 2022.167 kb on + strand, within RR42_RS29660 at 2022.168 kb on - strand, within RR42_RS29660 at 2022.168 kb on - strand, within RR42_RS29660 at 2022.168 kb on - strand, within RR42_RS29660 at 2022.205 kb on + strand, within RR42_RS29660 at 2022.206 kb on - strand, within RR42_RS29660 at 2022.206 kb on - strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.250 kb on + strand, within RR42_RS29660 at 2022.251 kb on - strand, within RR42_RS29660 at 2022.251 kb on - strand, within RR42_RS29660 at 2022.251 kb on - strand, within RR42_RS29660 at 2022.251 kb on - strand, within RR42_RS29660 at 2022.254 kb on + strand, within RR42_RS29660 at 2022.304 kb on + strand, within RR42_RS29660 at 2022.305 kb on - strand, within RR42_RS29660 at 2022.318 kb on - strand, within RR42_RS29660 at 2022.402 kb on + strand at 2022.403 kb on - strand at 2022.414 kb on + strand at 2022.414 kb on + strand at 2022.414 kb on + strand at 2022.468 kb on - strand at 2022.494 kb on + strand at 2022.529 kb on + strand at 2022.530 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 2,019,838 + -2.1 2,019,838 + +1.1 2,019,856 + -0.3 2,019,857 - +0.2 2,019,857 - +0.8 2,019,857 - -0.2 2,019,857 - -1.1 2,019,862 - +0.3 2,019,862 - -0.1 2,019,938 + -0.5 2,019,939 - +0.6 2,019,939 - -0.6 2,019,939 - +0.1 2,019,973 + +1.8 2,019,974 - -0.4 2,019,974 - +0.8 2,019,977 + +0.0 2,019,977 + -0.6 2,019,977 + +0.3 2,019,978 - +0.3 2,019,978 - -0.9 2,019,978 - +0.3 2,019,978 - -0.1 2,019,978 - -0.1 2,019,978 - +0.4 2,019,985 - -1.0 2,020,000 + -0.0 2,020,014 + +0.0 2,020,015 - +0.2 2,020,015 - +1.0 2,020,103 + +0.3 2,020,104 - -1.7 2,020,104 - +0.2 2,020,154 - -0.1 2,020,154 - -0.7 2,020,172 + +1.2 2,020,172 + -0.4 2,020,188 + +0.3 2,020,194 + -1.1 2,020,195 - -0.3 2,020,293 + RR42_RS29650 0.22 +0.1 2,020,294 - RR42_RS29650 0.22 -0.3 2,020,294 - RR42_RS29650 0.22 -0.8 2,020,294 - RR42_RS29650 0.22 +0.3 2,020,294 - RR42_RS29650 0.22 -0.2 2,020,294 - RR42_RS29650 0.22 -0.8 2,020,300 + RR42_RS29650 0.23 -0.8 2,020,301 - RR42_RS29650 0.23 +0.1 2,020,301 - RR42_RS29650 0.23 +0.3 2,020,311 + RR42_RS29650 0.24 +0.4 2,020,337 + RR42_RS29650 0.28 +0.4 2,020,337 + RR42_RS29650 0.28 +0.7 2,020,337 + RR42_RS29650 0.28 -0.5 2,020,439 + RR42_RS29650 0.43 +0.9 2,020,465 + RR42_RS29650 0.47 -0.1 2,020,466 - RR42_RS29650 0.47 -0.0 2,020,466 - RR42_RS29650 0.47 -1.1 2,020,470 + RR42_RS29650 0.47 +0.0 2,020,470 + RR42_RS29650 0.47 -1.6 2,020,470 + RR42_RS29650 0.47 +0.5 2,020,534 + RR42_RS29650 0.57 +0.9 2,020,535 - RR42_RS29650 0.57 -0.1 2,020,556 + RR42_RS29650 0.60 +0.1 2,020,557 - RR42_RS29650 0.60 -0.5 2,020,748 - RR42_RS29650 0.88 -0.5 2,020,748 - RR42_RS29650 0.88 -0.5 2,020,756 - RR42_RS29650 0.89 +0.3 2,020,801 + +0.0 2,020,801 + +0.8 2,020,802 - -0.8 2,020,802 - +0.4 2,020,831 + -0.0 2,020,832 - +0.7 2,020,845 + -0.1 2,020,877 - +0.4 2,020,951 - RR42_RS29655 0.16 +0.5 2,020,980 + RR42_RS29655 0.20 -0.9 2,020,980 + RR42_RS29655 0.20 +0.3 2,020,981 - RR42_RS29655 0.20 -0.5 2,021,021 - RR42_RS29655 0.26 -0.8 2,021,021 - RR42_RS29655 0.26 +0.2 2,021,026 + RR42_RS29655 0.26 +0.4 2,021,026 + RR42_RS29655 0.26 +0.9 2,021,026 + RR42_RS29655 0.26 +0.1 2,021,026 + RR42_RS29655 0.26 -0.8 2,021,027 - RR42_RS29655 0.27 +1.6 2,021,027 - RR42_RS29655 0.27 -0.5 2,021,027 - RR42_RS29655 0.27 -0.4 2,021,031 - RR42_RS29655 0.27 +0.6 2,021,031 - RR42_RS29655 0.27 -0.9 2,021,072 - RR42_RS29655 0.33 -0.2 2,021,072 - RR42_RS29655 0.33 -0.3 2,021,140 + RR42_RS29655 0.42 +0.0 2,021,143 + RR42_RS29655 0.43 +0.7 2,021,144 - RR42_RS29655 0.43 +0.4 2,021,144 - RR42_RS29655 0.43 -1.2 2,021,182 + RR42_RS29655 0.48 -0.5 2,021,276 - RR42_RS29655 0.61 -1.3 2,021,323 + RR42_RS29655 0.68 +0.5 2,021,324 - RR42_RS29655 0.68 +0.3 2,021,355 + RR42_RS29655 0.73 +0.1 2,021,355 + RR42_RS29655 0.73 +0.1 2,021,356 - RR42_RS29655 0.73 -0.4 2,021,356 - RR42_RS29655 0.73 +1.5 2,021,356 - RR42_RS29655 0.73 -0.5 2,021,356 - RR42_RS29655 0.73 -0.6 2,021,356 - RR42_RS29655 0.73 +1.2 2,021,425 + RR42_RS29655 0.82 +0.4 2,021,425 + RR42_RS29655 0.82 +0.7 2,021,425 + RR42_RS29655 0.82 -0.0 2,021,425 + RR42_RS29655 0.82 +1.4 2,021,426 - RR42_RS29655 0.82 -0.9 2,021,426 - RR42_RS29655 0.82 -0.0 2,021,426 - RR42_RS29655 0.82 +0.0 2,021,426 - RR42_RS29655 0.82 -2.0 2,021,426 - RR42_RS29655 0.82 +0.7 2,021,426 - RR42_RS29655 0.82 -1.1 2,021,464 + RR42_RS29655 0.88 +0.2 2,021,465 - RR42_RS29655 0.88 +0.0 2,021,528 - +0.0 2,021,528 - +0.7 2,021,528 - +1.3 2,021,548 + +0.3 2,021,548 + -1.0 2,021,549 - -0.1 2,021,549 - +0.1 2,021,615 - -2.1 2,021,615 - -0.6 2,021,689 + -2.0 2,021,689 + -0.3 2,021,689 + -0.2 2,021,726 + RR42_RS29660 0.15 +0.5 2,021,732 + RR42_RS29660 0.15 -0.9 2,021,732 + RR42_RS29660 0.15 -0.3 2,021,732 + RR42_RS29660 0.15 -1.2 2,022,033 + RR42_RS29660 0.53 +0.2 2,022,033 + RR42_RS29660 0.53 -0.9 2,022,034 - RR42_RS29660 0.53 +0.2 2,022,075 + RR42_RS29660 0.58 -0.3 2,022,076 - RR42_RS29660 0.59 -0.6 2,022,076 - RR42_RS29660 0.59 +0.4 2,022,076 - RR42_RS29660 0.59 +0.1 2,022,167 + RR42_RS29660 0.70 -0.6 2,022,167 + RR42_RS29660 0.70 -2.0 2,022,167 + RR42_RS29660 0.70 +0.3 2,022,168 - RR42_RS29660 0.70 +0.7 2,022,168 - RR42_RS29660 0.70 -0.0 2,022,168 - RR42_RS29660 0.70 +0.1 2,022,205 + RR42_RS29660 0.75 +0.9 2,022,206 - RR42_RS29660 0.75 +0.4 2,022,206 - RR42_RS29660 0.75 +0.0 2,022,250 + RR42_RS29660 0.81 -0.8 2,022,250 + RR42_RS29660 0.81 -0.2 2,022,250 + RR42_RS29660 0.81 +0.9 2,022,250 + RR42_RS29660 0.81 +0.2 2,022,250 + RR42_RS29660 0.81 -0.2 2,022,250 + RR42_RS29660 0.81 -0.3 2,022,251 - RR42_RS29660 0.81 +0.1 2,022,251 - RR42_RS29660 0.81 +1.0 2,022,251 - RR42_RS29660 0.81 -0.8 2,022,251 - RR42_RS29660 0.81 -0.2 2,022,254 + RR42_RS29660 0.81 -0.2 2,022,304 + RR42_RS29660 0.87 -0.4 2,022,305 - RR42_RS29660 0.87 +0.5 2,022,318 - RR42_RS29660 0.89 -0.4 2,022,402 + -0.6 2,022,403 - -1.3 2,022,414 + -0.6 2,022,414 + +0.9 2,022,414 + +0.9 2,022,468 - +0.7 2,022,494 + +0.8 2,022,529 + +1.1 2,022,530 - +0.4
Or see this region's nucleotide sequence