Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27740 and RR42_RS27745 are separated by 21 nucleotides RR42_RS27745 and RR42_RS27750 are separated by 127 nucleotides RR42_RS27750 and RR42_RS27755 are separated by 31 nucleotides
RR42_RS27740: RR42_RS27740 - fumarate hydratase, at 1,586,773 to 1,588,266
_RS27740
RR42_RS27745: RR42_RS27745 - hypothetical protein, at 1,588,288 to 1,588,515
_RS27745
RR42_RS27750: RR42_RS27750 - membrane protein, at 1,588,643 to 1,589,107
_RS27750
RR42_RS27755: RR42_RS27755 - rhombosortase, at 1,589,139 to 1,589,663
_RS27755
Position (kb)
1588
1589 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1587.426 kb on + strand, within RR42_RS27740 at 1587.426 kb on + strand, within RR42_RS27740 at 1587.427 kb on - strand, within RR42_RS27740 at 1587.560 kb on + strand, within RR42_RS27740 at 1587.560 kb on + strand, within RR42_RS27740 at 1587.848 kb on + strand, within RR42_RS27740 at 1587.849 kb on - strand, within RR42_RS27740 at 1587.904 kb on + strand, within RR42_RS27740 at 1587.904 kb on + strand, within RR42_RS27740 at 1587.904 kb on + strand, within RR42_RS27740 at 1587.905 kb on - strand, within RR42_RS27740 at 1587.907 kb on + strand, within RR42_RS27740 at 1587.907 kb on + strand, within RR42_RS27740 at 1587.908 kb on - strand, within RR42_RS27740 at 1587.960 kb on + strand, within RR42_RS27740 at 1587.961 kb on - strand, within RR42_RS27740 at 1588.087 kb on + strand, within RR42_RS27740 at 1588.114 kb on + strand, within RR42_RS27740 at 1588.183 kb on + strand at 1588.183 kb on + strand at 1588.183 kb on + strand at 1588.184 kb on - strand at 1588.184 kb on - strand at 1588.203 kb on + strand at 1588.204 kb on - strand at 1588.245 kb on - strand at 1588.245 kb on - strand at 1588.264 kb on + strand at 1588.265 kb on - strand at 1588.265 kb on - strand at 1588.289 kb on + strand at 1588.290 kb on - strand at 1588.290 kb on - strand at 1588.290 kb on - strand at 1588.424 kb on + strand, within RR42_RS27745 at 1588.425 kb on - strand, within RR42_RS27745 at 1588.425 kb on - strand, within RR42_RS27745 at 1588.460 kb on + strand, within RR42_RS27745 at 1588.460 kb on + strand, within RR42_RS27745 at 1588.461 kb on - strand, within RR42_RS27745 at 1588.561 kb on + strand at 1588.561 kb on + strand at 1588.562 kb on - strand at 1588.562 kb on - strand at 1588.892 kb on + strand, within RR42_RS27750 at 1588.892 kb on + strand, within RR42_RS27750 at 1588.892 kb on + strand, within RR42_RS27750 at 1588.892 kb on + strand, within RR42_RS27750 at 1588.893 kb on - strand, within RR42_RS27750 at 1588.893 kb on - strand, within RR42_RS27750 at 1588.919 kb on + strand, within RR42_RS27750 at 1588.919 kb on + strand, within RR42_RS27750 at 1588.962 kb on + strand, within RR42_RS27750 at 1588.962 kb on + strand, within RR42_RS27750 at 1588.963 kb on - strand, within RR42_RS27750 at 1588.979 kb on + strand, within RR42_RS27750 at 1588.997 kb on + strand, within RR42_RS27750 at 1588.998 kb on - strand, within RR42_RS27750 at 1589.052 kb on + strand, within RR42_RS27750 at 1589.053 kb on - strand, within RR42_RS27750 at 1589.053 kb on - strand, within RR42_RS27750 at 1589.066 kb on + strand at 1589.067 kb on - strand at 1589.106 kb on - strand at 1589.329 kb on + strand, within RR42_RS27755 at 1589.329 kb on + strand, within RR42_RS27755 at 1589.329 kb on + strand, within RR42_RS27755
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 1,587,426 + RR42_RS27740 0.44 +0.2 1,587,426 + RR42_RS27740 0.44 -1.4 1,587,427 - RR42_RS27740 0.44 +0.1 1,587,560 + RR42_RS27740 0.53 -0.3 1,587,560 + RR42_RS27740 0.53 +0.2 1,587,848 + RR42_RS27740 0.72 -0.7 1,587,849 - RR42_RS27740 0.72 +0.5 1,587,904 + RR42_RS27740 0.76 -0.5 1,587,904 + RR42_RS27740 0.76 -0.5 1,587,904 + RR42_RS27740 0.76 -0.4 1,587,905 - RR42_RS27740 0.76 +0.1 1,587,907 + RR42_RS27740 0.76 +0.0 1,587,907 + RR42_RS27740 0.76 +0.1 1,587,908 - RR42_RS27740 0.76 +0.2 1,587,960 + RR42_RS27740 0.79 -0.1 1,587,961 - RR42_RS27740 0.80 +0.1 1,588,087 + RR42_RS27740 0.88 +0.4 1,588,114 + RR42_RS27740 0.90 -0.0 1,588,183 + +0.3 1,588,183 + -0.0 1,588,183 + -0.2 1,588,184 - +0.4 1,588,184 - -1.0 1,588,203 + -0.5 1,588,204 - -0.4 1,588,245 - -0.5 1,588,245 - +0.4 1,588,264 + +1.3 1,588,265 - +0.3 1,588,265 - -0.1 1,588,289 + +0.3 1,588,290 - +0.3 1,588,290 - -1.8 1,588,290 - +0.0 1,588,424 + RR42_RS27745 0.60 +0.5 1,588,425 - RR42_RS27745 0.60 -0.0 1,588,425 - RR42_RS27745 0.60 +0.8 1,588,460 + RR42_RS27745 0.75 +0.4 1,588,460 + RR42_RS27745 0.75 +0.4 1,588,461 - RR42_RS27745 0.76 +0.5 1,588,561 + +0.0 1,588,561 + +0.2 1,588,562 - +2.5 1,588,562 - -1.0 1,588,892 + RR42_RS27750 0.54 +0.1 1,588,892 + RR42_RS27750 0.54 +0.0 1,588,892 + RR42_RS27750 0.54 +0.4 1,588,892 + RR42_RS27750 0.54 -0.3 1,588,893 - RR42_RS27750 0.54 +0.4 1,588,893 - RR42_RS27750 0.54 +0.1 1,588,919 + RR42_RS27750 0.59 -1.2 1,588,919 + RR42_RS27750 0.59 +0.1 1,588,962 + RR42_RS27750 0.69 -0.2 1,588,962 + RR42_RS27750 0.69 -0.6 1,588,963 - RR42_RS27750 0.69 -0.0 1,588,979 + RR42_RS27750 0.72 +0.7 1,588,997 + RR42_RS27750 0.76 -0.4 1,588,998 - RR42_RS27750 0.76 +0.1 1,589,052 + RR42_RS27750 0.88 -0.9 1,589,053 - RR42_RS27750 0.88 -0.1 1,589,053 - RR42_RS27750 0.88 -0.5 1,589,066 + -0.0 1,589,067 - +0.7 1,589,106 - -0.1 1,589,329 + RR42_RS27755 0.36 -0.1 1,589,329 + RR42_RS27755 0.36 -0.4 1,589,329 + RR42_RS27755 0.36 +0.9
Or see this region's nucleotide sequence