Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20935 and RR42_RS20940 are separated by 87 nucleotides RR42_RS20940 and RR42_RS20945 overlap by 4 nucleotides RR42_RS20945 and RR42_RS20950 are separated by 11 nucleotides
RR42_RS20935: RR42_RS20935 - hydrolase, at 22,122 to 23,990
_RS20935
RR42_RS20940: RR42_RS20940 - tyrosine protein kinase, at 24,078 to 26,321
_RS20940
RR42_RS20945: RR42_RS20945 - protein-tyrosine phosphatase, at 26,318 to 26,782
_RS20945
RR42_RS20950: RR42_RS20950 - multidrug MFS transporter, at 26,794 to 27,888
_RS20950
Position (kb) 24
25
26
27 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.241 kb on + strand, within RR42_RS20935 at 23.302 kb on - strand, within RR42_RS20935 at 23.470 kb on - strand, within RR42_RS20935 at 23.700 kb on + strand, within RR42_RS20935 at 23.865 kb on + strand at 23.865 kb on + strand at 23.866 kb on - strand at 23.969 kb on - strand at 24.115 kb on + strand at 24.115 kb on + strand at 24.116 kb on - strand at 24.130 kb on + strand at 24.130 kb on + strand at 24.131 kb on - strand at 24.146 kb on - strand at 24.364 kb on + strand, within RR42_RS20940 at 24.365 kb on - strand, within RR42_RS20940 at 24.564 kb on - strand, within RR42_RS20940 at 24.847 kb on + strand, within RR42_RS20940 at 24.847 kb on + strand, within RR42_RS20940 at 24.848 kb on - strand, within RR42_RS20940 at 24.848 kb on - strand, within RR42_RS20940 at 24.875 kb on + strand, within RR42_RS20940 at 24.974 kb on + strand, within RR42_RS20940 at 25.682 kb on + strand, within RR42_RS20940 at 25.856 kb on + strand, within RR42_RS20940 at 25.924 kb on + strand, within RR42_RS20940 at 25.924 kb on + strand, within RR42_RS20940 at 25.924 kb on + strand, within RR42_RS20940 at 25.925 kb on - strand, within RR42_RS20940 at 25.957 kb on + strand, within RR42_RS20940 at 25.958 kb on - strand, within RR42_RS20940 at 26.324 kb on + strand at 26.419 kb on + strand, within RR42_RS20945 at 26.419 kb on + strand, within RR42_RS20945 at 26.420 kb on - strand, within RR42_RS20945 at 26.420 kb on - strand, within RR42_RS20945 at 26.638 kb on + strand, within RR42_RS20945 at 26.892 kb on - strand at 26.967 kb on - strand, within RR42_RS20950 at 27.109 kb on + strand, within RR42_RS20950 at 27.109 kb on + strand, within RR42_RS20950 at 27.110 kb on - strand, within RR42_RS20950
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 23,241 + RR42_RS20935 0.60 -0.4 23,302 - RR42_RS20935 0.63 +0.3 23,470 - RR42_RS20935 0.72 -0.1 23,700 + RR42_RS20935 0.84 -0.8 23,865 + -1.0 23,865 + +1.9 23,866 - -1.4 23,969 - -0.4 24,115 + -0.1 24,115 + -0.1 24,116 - +0.0 24,130 + -1.5 24,130 + +0.0 24,131 - -0.6 24,146 - +1.0 24,364 + RR42_RS20940 0.13 +1.8 24,365 - RR42_RS20940 0.13 -1.0 24,564 - RR42_RS20940 0.22 -0.8 24,847 + RR42_RS20940 0.34 -0.2 24,847 + RR42_RS20940 0.34 +0.1 24,848 - RR42_RS20940 0.34 +0.0 24,848 - RR42_RS20940 0.34 -0.1 24,875 + RR42_RS20940 0.36 -1.6 24,974 + RR42_RS20940 0.40 +2.6 25,682 + RR42_RS20940 0.71 -0.2 25,856 + RR42_RS20940 0.79 -0.5 25,924 + RR42_RS20940 0.82 -1.4 25,924 + RR42_RS20940 0.82 -0.1 25,924 + RR42_RS20940 0.82 +0.5 25,925 - RR42_RS20940 0.82 +0.1 25,957 + RR42_RS20940 0.84 +0.1 25,958 - RR42_RS20940 0.84 -1.5 26,324 + -1.1 26,419 + RR42_RS20945 0.22 -1.4 26,419 + RR42_RS20945 0.22 +0.1 26,420 - RR42_RS20945 0.22 -0.2 26,420 - RR42_RS20945 0.22 +0.5 26,638 + RR42_RS20945 0.69 +1.4 26,892 - +1.0 26,967 - RR42_RS20950 0.16 +0.5 27,109 + RR42_RS20950 0.29 +0.2 27,109 + RR42_RS20950 0.29 -1.8 27,110 - RR42_RS20950 0.29 +0.2
Or see this region's nucleotide sequence