Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17390 and RR42_RS17395 are separated by 184 nucleotides RR42_RS17395 and RR42_RS17400 are separated by 40 nucleotides
RR42_RS17390: RR42_RS17390 - ATP-dependent DNA helicase RecG, at 3,762,702 to 3,764,903
_RS17390
RR42_RS17395: RR42_RS17395 - S-adenosylmethionine tRNA ribosyltransferase, at 3,765,088 to 3,766,140
_RS17395
RR42_RS17400: RR42_RS17400 - queuine tRNA-ribosyltransferase, at 3,766,181 to 3,767,332
_RS17400
Position (kb)
3765
3766
3767 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3764.344 kb on + strand, within RR42_RS17390 at 3764.757 kb on - strand at 3764.975 kb on + strand at 3765.012 kb on + strand at 3765.013 kb on + strand at 3765.013 kb on + strand at 3765.013 kb on + strand at 3765.013 kb on + strand at 3765.014 kb on - strand at 3765.014 kb on - strand at 3765.014 kb on - strand at 3765.014 kb on - strand at 3765.036 kb on + strand at 3765.036 kb on + strand at 3765.037 kb on - strand at 3765.114 kb on + strand at 3765.115 kb on - strand at 3765.247 kb on + strand, within RR42_RS17395 at 3765.248 kb on - strand, within RR42_RS17395 at 3765.248 kb on - strand, within RR42_RS17395 at 3765.386 kb on + strand, within RR42_RS17395 at 3765.386 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.544 kb on + strand, within RR42_RS17395 at 3765.545 kb on - strand, within RR42_RS17395 at 3765.545 kb on - strand, within RR42_RS17395 at 3765.545 kb on - strand, within RR42_RS17395 at 3765.545 kb on - strand, within RR42_RS17395 at 3765.778 kb on + strand, within RR42_RS17395 at 3766.093 kb on + strand at 3766.093 kb on + strand at 3766.286 kb on + strand at 3766.286 kb on + strand at 3766.286 kb on + strand at 3766.286 kb on + strand at 3766.286 kb on + strand at 3766.287 kb on - strand at 3766.287 kb on - strand at 3766.826 kb on + strand, within RR42_RS17400 at 3767.111 kb on + strand, within RR42_RS17400 at 3767.111 kb on + strand, within RR42_RS17400 at 3767.112 kb on - strand, within RR42_RS17400 at 3767.112 kb on - strand, within RR42_RS17400 at 3767.112 kb on - strand, within RR42_RS17400 at 3767.113 kb on + strand, within RR42_RS17400 at 3767.113 kb on + strand, within RR42_RS17400 at 3767.114 kb on - strand, within RR42_RS17400 at 3767.114 kb on - strand, within RR42_RS17400 at 3767.138 kb on + strand, within RR42_RS17400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 3,764,344 + RR42_RS17390 0.75 -2.7 3,764,757 - -0.8 3,764,975 + -0.2 3,765,012 + -0.4 3,765,013 + +0.5 3,765,013 + -1.8 3,765,013 + +0.1 3,765,013 + +0.1 3,765,014 - +0.5 3,765,014 - +0.5 3,765,014 - -0.5 3,765,014 - -0.1 3,765,036 + -0.4 3,765,036 + -0.7 3,765,037 - -2.8 3,765,114 + -1.4 3,765,115 - -0.3 3,765,247 + RR42_RS17395 0.15 -0.7 3,765,248 - RR42_RS17395 0.15 -2.3 3,765,248 - RR42_RS17395 0.15 -1.6 3,765,386 + RR42_RS17395 0.28 -1.7 3,765,386 + RR42_RS17395 0.28 +0.3 3,765,544 + RR42_RS17395 0.43 -0.3 3,765,544 + RR42_RS17395 0.43 -0.4 3,765,544 + RR42_RS17395 0.43 -1.0 3,765,544 + RR42_RS17395 0.43 -0.2 3,765,544 + RR42_RS17395 0.43 +0.2 3,765,544 + RR42_RS17395 0.43 -0.7 3,765,545 - RR42_RS17395 0.43 +1.1 3,765,545 - RR42_RS17395 0.43 -1.6 3,765,545 - RR42_RS17395 0.43 -1.0 3,765,545 - RR42_RS17395 0.43 -1.5 3,765,778 + RR42_RS17395 0.66 -1.0 3,766,093 + -0.4 3,766,093 + -0.6 3,766,286 + -0.6 3,766,286 + -2.0 3,766,286 + -1.1 3,766,286 + -0.5 3,766,286 + -2.2 3,766,287 - -1.3 3,766,287 - -0.2 3,766,826 + RR42_RS17400 0.56 +0.5 3,767,111 + RR42_RS17400 0.81 -0.8 3,767,111 + RR42_RS17400 0.81 -0.1 3,767,112 - RR42_RS17400 0.81 -0.1 3,767,112 - RR42_RS17400 0.81 -1.3 3,767,112 - RR42_RS17400 0.81 +0.7 3,767,113 + RR42_RS17400 0.81 -0.8 3,767,113 + RR42_RS17400 0.81 -0.4 3,767,114 - RR42_RS17400 0.81 +0.4 3,767,114 - RR42_RS17400 0.81 -1.3 3,767,138 + RR42_RS17400 0.83 -1.0
Or see this region's nucleotide sequence