Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10670 and RR42_RS10675 are separated by 198 nucleotides RR42_RS10675 and RR42_RS10680 are separated by 46 nucleotides RR42_RS10680 and RR42_RS10685 overlap by 4 nucleotides RR42_RS10685 and RR42_RS10690 are separated by 652 nucleotides
RR42_RS10670: RR42_RS10670 - phosphopantetheine-binding protein, at 2,279,947 to 2,280,195
_RS10670
RR42_RS10675: RR42_RS10675 - hypothetical protein, at 2,280,394 to 2,280,699
_RS10675
RR42_RS10680: RR42_RS10680 - extradiol dioxygenase, at 2,280,746 to 2,281,162
_RS10680
RR42_RS10685: RR42_RS10685 - 3-oxoacyl-ACP reductase, at 2,281,159 to 2,281,626
_RS10685
RR42_RS10690: RR42_RS10690 - 3-oxoadipate enol-lactonase, at 2,282,279 to 2,283,058
_RS10690
Position (kb)
2281
2282 Strain fitness (log2 ratio)
-1
0
1
2 at 2280.170 kb on - strand, within RR42_RS10670 at 2280.172 kb on - strand at 2280.194 kb on - strand at 2280.194 kb on - strand at 2280.199 kb on + strand at 2280.199 kb on + strand at 2280.199 kb on + strand at 2280.200 kb on - strand at 2280.202 kb on + strand at 2280.202 kb on + strand at 2280.203 kb on - strand at 2280.203 kb on - strand at 2280.203 kb on - strand at 2280.314 kb on + strand at 2280.314 kb on + strand at 2280.315 kb on - strand at 2280.357 kb on + strand at 2280.357 kb on + strand at 2280.357 kb on + strand at 2280.439 kb on + strand, within RR42_RS10675 at 2280.576 kb on + strand, within RR42_RS10675 at 2280.577 kb on - strand, within RR42_RS10675 at 2280.577 kb on - strand, within RR42_RS10675 at 2280.772 kb on - strand at 2280.783 kb on - strand at 2280.790 kb on - strand, within RR42_RS10680 at 2280.822 kb on + strand, within RR42_RS10680 at 2280.823 kb on - strand, within RR42_RS10680 at 2280.834 kb on + strand, within RR42_RS10680 at 2280.895 kb on - strand, within RR42_RS10680 at 2280.961 kb on + strand, within RR42_RS10680 at 2280.984 kb on - strand, within RR42_RS10680 at 2281.047 kb on + strand, within RR42_RS10680 at 2281.047 kb on + strand, within RR42_RS10680 at 2281.092 kb on + strand, within RR42_RS10680 at 2281.093 kb on - strand, within RR42_RS10680 at 2281.093 kb on - strand, within RR42_RS10680 at 2281.093 kb on - strand, within RR42_RS10680 at 2281.093 kb on - strand, within RR42_RS10680 at 2281.169 kb on + strand at 2281.295 kb on - strand, within RR42_RS10685 at 2281.363 kb on - strand, within RR42_RS10685 at 2281.400 kb on + strand, within RR42_RS10685 at 2281.401 kb on - strand, within RR42_RS10685 at 2281.429 kb on - strand, within RR42_RS10685 at 2281.611 kb on - strand at 2281.619 kb on + strand at 2281.620 kb on - strand at 2281.645 kb on - strand at 2281.691 kb on - strand at 2281.696 kb on + strand at 2281.886 kb on - strand at 2281.984 kb on + strand at 2282.016 kb on + strand at 2282.083 kb on + strand at 2282.083 kb on + strand at 2282.084 kb on - strand at 2282.085 kb on + strand at 2282.085 kb on + strand at 2282.136 kb on - strand at 2282.158 kb on - strand at 2282.158 kb on - strand at 2282.259 kb on - strand at 2282.259 kb on - strand at 2282.395 kb on - strand, within RR42_RS10690 at 2282.395 kb on - strand, within RR42_RS10690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 2,280,170 - RR42_RS10670 0.90 +0.8 2,280,172 - +0.4 2,280,194 - -0.1 2,280,194 - -0.5 2,280,199 + -0.2 2,280,199 + -0.9 2,280,199 + -1.4 2,280,200 - -0.4 2,280,202 + -0.5 2,280,202 + -0.1 2,280,203 - +0.6 2,280,203 - -0.1 2,280,203 - -0.6 2,280,314 + -0.5 2,280,314 + +0.7 2,280,315 - +0.3 2,280,357 + +0.3 2,280,357 + +0.8 2,280,357 + +0.5 2,280,439 + RR42_RS10675 0.15 +0.2 2,280,576 + RR42_RS10675 0.59 -0.1 2,280,577 - RR42_RS10675 0.60 -0.5 2,280,577 - RR42_RS10675 0.60 -1.0 2,280,772 - +0.3 2,280,783 - -0.3 2,280,790 - RR42_RS10680 0.11 -1.0 2,280,822 + RR42_RS10680 0.18 +0.1 2,280,823 - RR42_RS10680 0.18 -0.5 2,280,834 + RR42_RS10680 0.21 -0.1 2,280,895 - RR42_RS10680 0.36 +0.5 2,280,961 + RR42_RS10680 0.52 -0.3 2,280,984 - RR42_RS10680 0.57 -0.0 2,281,047 + RR42_RS10680 0.72 -0.3 2,281,047 + RR42_RS10680 0.72 -0.4 2,281,092 + RR42_RS10680 0.83 -0.5 2,281,093 - RR42_RS10680 0.83 -0.9 2,281,093 - RR42_RS10680 0.83 -0.5 2,281,093 - RR42_RS10680 0.83 +0.3 2,281,093 - RR42_RS10680 0.83 -0.5 2,281,169 + -1.6 2,281,295 - RR42_RS10685 0.29 -0.8 2,281,363 - RR42_RS10685 0.44 +0.0 2,281,400 + RR42_RS10685 0.51 -1.1 2,281,401 - RR42_RS10685 0.52 -0.6 2,281,429 - RR42_RS10685 0.58 -1.1 2,281,611 - -0.7 2,281,619 + +0.3 2,281,620 - -0.8 2,281,645 - +0.6 2,281,691 - +0.4 2,281,696 + -0.7 2,281,886 - -0.1 2,281,984 + -0.6 2,282,016 + +0.4 2,282,083 + +0.1 2,282,083 + +0.1 2,282,084 - -1.0 2,282,085 + +0.2 2,282,085 + -0.7 2,282,136 - -0.7 2,282,158 - +0.0 2,282,158 - -0.1 2,282,259 - +2.7 2,282,259 - +0.1 2,282,395 - RR42_RS10690 0.15 +0.2 2,282,395 - RR42_RS10690 0.15 -0.2
Or see this region's nucleotide sequence