Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09080 and RR42_RS09085 overlap by 1 nucleotides RR42_RS09085 and RR42_RS09090 are separated by 223 nucleotides RR42_RS09090 and RR42_RS09095 are separated by 150 nucleotides
RR42_RS09080: RR42_RS09080 - C4-dicarboxylate ABC transporter permease, at 1,910,423 to 1,911,733
_RS09080
RR42_RS09085: RR42_RS09085 - C4-dicarboxylate ABC transporter permease, at 1,911,733 to 1,912,284
_RS09085
RR42_RS09090: RR42_RS09090 - C4-dicarboxylate ABC transporter substrate-binding protein, at 1,912,508 to 1,913,467
_RS09090
RR42_RS09095: RR42_RS09095 - amidase, at 1,913,618 to 1,914,961
_RS09095
Position (kb)
1912
1913
1914 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.557 kb on - strand, within RR42_RS09080 at 1912.094 kb on - strand, within RR42_RS09085 at 1912.152 kb on + strand, within RR42_RS09085 at 1912.392 kb on + strand at 1912.393 kb on - strand at 1912.413 kb on + strand at 1912.425 kb on + strand at 1912.461 kb on + strand at 1912.474 kb on - strand at 1912.519 kb on - strand at 1912.663 kb on - strand, within RR42_RS09090 at 1912.815 kb on + strand, within RR42_RS09090 at 1912.815 kb on + strand, within RR42_RS09090 at 1912.816 kb on - strand, within RR42_RS09090 at 1912.980 kb on + strand, within RR42_RS09090 at 1912.980 kb on + strand, within RR42_RS09090 at 1912.981 kb on - strand, within RR42_RS09090 at 1912.981 kb on - strand, within RR42_RS09090 at 1912.981 kb on - strand, within RR42_RS09090 at 1913.038 kb on - strand, within RR42_RS09090 at 1913.068 kb on - strand, within RR42_RS09090 at 1913.068 kb on - strand, within RR42_RS09090 at 1913.136 kb on + strand, within RR42_RS09090 at 1913.136 kb on + strand, within RR42_RS09090 at 1913.137 kb on - strand, within RR42_RS09090 at 1913.137 kb on - strand, within RR42_RS09090 at 1913.148 kb on + strand, within RR42_RS09090 at 1913.186 kb on + strand, within RR42_RS09090 at 1913.779 kb on + strand, within RR42_RS09095 at 1913.779 kb on + strand, within RR42_RS09095 at 1913.950 kb on - strand, within RR42_RS09095 at 1913.950 kb on - strand, within RR42_RS09095 at 1913.950 kb on - strand, within RR42_RS09095 at 1914.007 kb on - strand, within RR42_RS09095 at 1914.341 kb on - strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 1,911,516 - RR42_RS09080 0.83 +0.2 1,911,516 - RR42_RS09080 0.83 +0.6 1,911,516 - RR42_RS09080 0.83 -0.9 1,911,557 - RR42_RS09080 0.86 -0.6 1,912,094 - RR42_RS09085 0.65 -0.3 1,912,152 + RR42_RS09085 0.76 -1.6 1,912,392 + +0.6 1,912,393 - +0.2 1,912,413 + +0.3 1,912,425 + +0.8 1,912,461 + -0.6 1,912,474 - -0.1 1,912,519 - +0.5 1,912,663 - RR42_RS09090 0.16 +0.9 1,912,815 + RR42_RS09090 0.32 -0.7 1,912,815 + RR42_RS09090 0.32 -0.4 1,912,816 - RR42_RS09090 0.32 +0.0 1,912,980 + RR42_RS09090 0.49 +0.7 1,912,980 + RR42_RS09090 0.49 -0.4 1,912,981 - RR42_RS09090 0.49 +2.3 1,912,981 - RR42_RS09090 0.49 +0.1 1,912,981 - RR42_RS09090 0.49 +0.6 1,913,038 - RR42_RS09090 0.55 +0.0 1,913,068 - RR42_RS09090 0.58 +0.3 1,913,068 - RR42_RS09090 0.58 +1.2 1,913,136 + RR42_RS09090 0.65 +0.2 1,913,136 + RR42_RS09090 0.65 +1.4 1,913,137 - RR42_RS09090 0.66 +1.8 1,913,137 - RR42_RS09090 0.66 +0.4 1,913,148 + RR42_RS09090 0.67 -0.8 1,913,186 + RR42_RS09090 0.71 -0.9 1,913,779 + RR42_RS09095 0.12 +1.8 1,913,779 + RR42_RS09095 0.12 -0.4 1,913,950 - RR42_RS09095 0.25 +0.8 1,913,950 - RR42_RS09095 0.25 -0.2 1,913,950 - RR42_RS09095 0.25 -0.9 1,914,007 - RR42_RS09095 0.29 +0.2 1,914,341 - RR42_RS09095 0.54 +0.5 1,914,342 + RR42_RS09095 0.54 -1.2 1,914,342 + RR42_RS09095 0.54 +0.3 1,914,342 + RR42_RS09095 0.54 -2.2
Or see this region's nucleotide sequence