Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08855 and RR42_RS08860 are separated by 43 nucleotides RR42_RS08860 and RR42_RS08865 are separated by 190 nucleotides RR42_RS08865 and RR42_RS08870 are separated by 50 nucleotides
RR42_RS08855: RR42_RS08855 - hypothetical protein, at 1,861,007 to 1,861,207
_RS08855
RR42_RS08860: RR42_RS08860 - radical SAM protein, at 1,861,251 to 1,861,910
_RS08860
RR42_RS08865: RR42_RS08865 - hydroxylase, at 1,862,101 to 1,862,388
_RS08865
RR42_RS08870: RR42_RS08870 - hypothetical protein, at 1,862,439 to 1,863,524
_RS08870
Position (kb)
1862
1863 Strain fitness (log2 ratio)
-2
-1
0
1 at 1861.115 kb on + strand, within RR42_RS08855 at 1861.116 kb on - strand, within RR42_RS08855 at 1861.210 kb on + strand at 1861.210 kb on + strand at 1861.211 kb on - strand at 1861.347 kb on + strand, within RR42_RS08860 at 1861.348 kb on - strand, within RR42_RS08860 at 1861.348 kb on - strand, within RR42_RS08860 at 1861.554 kb on + strand, within RR42_RS08860 at 1861.854 kb on + strand at 1861.931 kb on + strand at 1861.931 kb on + strand at 1862.015 kb on + strand at 1862.016 kb on - strand at 1862.016 kb on - strand at 1862.016 kb on - strand at 1862.177 kb on - strand, within RR42_RS08865 at 1862.177 kb on - strand, within RR42_RS08865 at 1862.321 kb on + strand, within RR42_RS08865 at 1862.532 kb on + strand at 1863.048 kb on + strand, within RR42_RS08870 at 1863.048 kb on + strand, within RR42_RS08870 at 1863.049 kb on - strand, within RR42_RS08870 at 1863.049 kb on - strand, within RR42_RS08870 at 1863.136 kb on - strand, within RR42_RS08870 at 1863.136 kb on - strand, within RR42_RS08870 at 1863.311 kb on + strand, within RR42_RS08870 at 1863.339 kb on + strand, within RR42_RS08870 at 1863.339 kb on + strand, within RR42_RS08870 at 1863.340 kb on - strand, within RR42_RS08870 at 1863.340 kb on - strand, within RR42_RS08870
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 1,861,115 + RR42_RS08855 0.54 -0.2 1,861,116 - RR42_RS08855 0.54 -0.3 1,861,210 + -0.1 1,861,210 + +0.1 1,861,211 - -0.5 1,861,347 + RR42_RS08860 0.15 +0.0 1,861,348 - RR42_RS08860 0.15 -0.1 1,861,348 - RR42_RS08860 0.15 -0.0 1,861,554 + RR42_RS08860 0.46 -0.8 1,861,854 + -1.3 1,861,931 + -0.0 1,861,931 + -0.9 1,862,015 + +0.4 1,862,016 - +0.9 1,862,016 - +0.8 1,862,016 - -1.9 1,862,177 - RR42_RS08865 0.26 -0.8 1,862,177 - RR42_RS08865 0.26 +0.3 1,862,321 + RR42_RS08865 0.76 -0.8 1,862,532 + +0.4 1,863,048 + RR42_RS08870 0.56 -0.8 1,863,048 + RR42_RS08870 0.56 +0.0 1,863,049 - RR42_RS08870 0.56 +1.2 1,863,049 - RR42_RS08870 0.56 +0.6 1,863,136 - RR42_RS08870 0.64 -0.1 1,863,136 - RR42_RS08870 0.64 +0.4 1,863,311 + RR42_RS08870 0.80 +0.0 1,863,339 + RR42_RS08870 0.83 +1.1 1,863,339 + RR42_RS08870 0.83 +0.0 1,863,340 - RR42_RS08870 0.83 -0.4 1,863,340 - RR42_RS08870 0.83 +0.1
Or see this region's nucleotide sequence