Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07530 and RR42_RS07535 are separated by 110 nucleotides RR42_RS07535 and RR42_RS07540 are separated by 4 nucleotides RR42_RS07540 and RR42_RS07545 overlap by 4 nucleotides RR42_RS07545 and RR42_RS07550 overlap by 4 nucleotides
RR42_RS07530: RR42_RS07530 - ABC transporter permease, at 1,609,529 to 1,610,710
_RS07530
RR42_RS07535: RR42_RS07535 - ABC transporter permease, at 1,610,821 to 1,611,687
_RS07535
RR42_RS07540: RR42_RS07540 - ABC transporter permease, at 1,611,692 to 1,612,717
_RS07540
RR42_RS07545: RR42_RS07545 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,612,714 to 1,613,562
_RS07545
RR42_RS07550: RR42_RS07550 - ABC transporter, at 1,613,559 to 1,614,278
_RS07550
Position (kb)
1611
1612
1613 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1610.716 kb on + strand at 1610.856 kb on + strand at 1610.856 kb on + strand at 1610.856 kb on + strand at 1610.866 kb on + strand at 1610.953 kb on + strand, within RR42_RS07535 at 1610.953 kb on + strand, within RR42_RS07535 at 1610.953 kb on + strand, within RR42_RS07535 at 1610.953 kb on + strand, within RR42_RS07535 at 1610.953 kb on + strand, within RR42_RS07535 at 1610.953 kb on + strand, within RR42_RS07535 at 1610.954 kb on - strand, within RR42_RS07535 at 1610.954 kb on - strand, within RR42_RS07535 at 1611.079 kb on + strand, within RR42_RS07535 at 1611.080 kb on - strand, within RR42_RS07535 at 1611.080 kb on - strand, within RR42_RS07535 at 1611.080 kb on - strand, within RR42_RS07535 at 1611.080 kb on - strand, within RR42_RS07535 at 1611.085 kb on + strand, within RR42_RS07535 at 1611.086 kb on - strand, within RR42_RS07535 at 1611.121 kb on + strand, within RR42_RS07535 at 1611.122 kb on - strand, within RR42_RS07535 at 1611.176 kb on + strand, within RR42_RS07535 at 1611.176 kb on + strand, within RR42_RS07535 at 1611.176 kb on + strand, within RR42_RS07535 at 1611.176 kb on + strand, within RR42_RS07535 at 1611.176 kb on + strand, within RR42_RS07535 at 1611.177 kb on - strand, within RR42_RS07535 at 1611.177 kb on - strand, within RR42_RS07535 at 1611.177 kb on - strand, within RR42_RS07535 at 1611.239 kb on - strand, within RR42_RS07535 at 1611.248 kb on - strand, within RR42_RS07535 at 1611.295 kb on + strand, within RR42_RS07535 at 1611.296 kb on - strand, within RR42_RS07535 at 1611.296 kb on - strand, within RR42_RS07535 at 1611.394 kb on + strand, within RR42_RS07535 at 1611.583 kb on - strand, within RR42_RS07535 at 1611.629 kb on - strand at 1611.704 kb on - strand at 1611.807 kb on + strand, within RR42_RS07540 at 1611.808 kb on - strand, within RR42_RS07540 at 1611.906 kb on - strand, within RR42_RS07540 at 1611.938 kb on + strand, within RR42_RS07540 at 1612.007 kb on - strand, within RR42_RS07540 at 1612.085 kb on + strand, within RR42_RS07540 at 1612.085 kb on + strand, within RR42_RS07540 at 1612.099 kb on + strand, within RR42_RS07540 at 1612.124 kb on + strand, within RR42_RS07540 at 1612.124 kb on + strand, within RR42_RS07540 at 1612.124 kb on + strand, within RR42_RS07540 at 1612.124 kb on - strand, within RR42_RS07540 at 1612.125 kb on - strand, within RR42_RS07540 at 1612.125 kb on - strand, within RR42_RS07540 at 1612.125 kb on - strand, within RR42_RS07540 at 1612.172 kb on + strand, within RR42_RS07540 at 1612.172 kb on + strand, within RR42_RS07540 at 1612.172 kb on + strand, within RR42_RS07540 at 1612.172 kb on + strand, within RR42_RS07540 at 1612.172 kb on + strand, within RR42_RS07540 at 1612.172 kb on + strand, within RR42_RS07540 at 1612.172 kb on + strand, within RR42_RS07540 at 1612.172 kb on + strand, within RR42_RS07540 at 1612.173 kb on - strand, within RR42_RS07540 at 1612.173 kb on - strand, within RR42_RS07540 at 1612.173 kb on - strand, within RR42_RS07540 at 1612.173 kb on - strand, within RR42_RS07540 at 1612.173 kb on - strand, within RR42_RS07540 at 1612.173 kb on - strand, within RR42_RS07540 at 1612.173 kb on - strand, within RR42_RS07540 at 1612.174 kb on + strand, within RR42_RS07540 at 1612.175 kb on - strand, within RR42_RS07540 at 1612.175 kb on - strand, within RR42_RS07540 at 1612.175 kb on - strand, within RR42_RS07540 at 1612.175 kb on - strand, within RR42_RS07540 at 1612.175 kb on - strand, within RR42_RS07540 at 1612.175 kb on - strand, within RR42_RS07540 at 1612.179 kb on - strand, within RR42_RS07540 at 1612.179 kb on - strand, within RR42_RS07540 at 1612.244 kb on + strand, within RR42_RS07540 at 1612.245 kb on - strand, within RR42_RS07540 at 1612.294 kb on - strand, within RR42_RS07540 at 1612.340 kb on - strand, within RR42_RS07540 at 1612.376 kb on + strand, within RR42_RS07540 at 1612.376 kb on + strand, within RR42_RS07540 at 1612.376 kb on + strand, within RR42_RS07540 at 1612.376 kb on + strand, within RR42_RS07540 at 1612.376 kb on + strand, within RR42_RS07540 at 1612.376 kb on + strand, within RR42_RS07540 at 1612.377 kb on - strand, within RR42_RS07540 at 1612.377 kb on - strand, within RR42_RS07540 at 1612.434 kb on - strand, within RR42_RS07540 at 1612.439 kb on + strand, within RR42_RS07540 at 1612.439 kb on + strand, within RR42_RS07540 at 1612.439 kb on + strand, within RR42_RS07540 at 1612.440 kb on - strand, within RR42_RS07540 at 1612.440 kb on - strand, within RR42_RS07540 at 1612.881 kb on + strand, within RR42_RS07545 at 1612.881 kb on + strand, within RR42_RS07545 at 1612.881 kb on + strand, within RR42_RS07545 at 1612.882 kb on - strand, within RR42_RS07545 at 1612.882 kb on - strand, within RR42_RS07545 at 1612.882 kb on - strand, within RR42_RS07545 at 1612.882 kb on - strand, within RR42_RS07545 at 1612.882 kb on - strand, within RR42_RS07545 at 1612.882 kb on - strand, within RR42_RS07545 at 1613.004 kb on + strand, within RR42_RS07545 at 1613.005 kb on - strand, within RR42_RS07545 at 1613.005 kb on - strand, within RR42_RS07545 at 1613.465 kb on - strand, within RR42_RS07545 at 1613.465 kb on - strand, within RR42_RS07545 at 1613.545 kb on - strand at 1613.607 kb on + strand at 1613.608 kb on - strand at 1613.608 kb on - strand at 1613.711 kb on + strand, within RR42_RS07550 at 1613.711 kb on + strand, within RR42_RS07550 at 1613.712 kb on - strand, within RR42_RS07550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 1,610,716 + -0.3 1,610,856 + +0.4 1,610,856 + -0.1 1,610,856 + -0.7 1,610,866 + +0.3 1,610,953 + RR42_RS07535 0.15 +0.8 1,610,953 + RR42_RS07535 0.15 +0.3 1,610,953 + RR42_RS07535 0.15 +0.1 1,610,953 + RR42_RS07535 0.15 +2.1 1,610,953 + RR42_RS07535 0.15 +0.1 1,610,953 + RR42_RS07535 0.15 +0.0 1,610,954 - RR42_RS07535 0.15 -0.1 1,610,954 - RR42_RS07535 0.15 -0.8 1,611,079 + RR42_RS07535 0.30 +0.2 1,611,080 - RR42_RS07535 0.30 +0.5 1,611,080 - RR42_RS07535 0.30 +0.1 1,611,080 - RR42_RS07535 0.30 -0.2 1,611,080 - RR42_RS07535 0.30 +0.3 1,611,085 + RR42_RS07535 0.30 -0.0 1,611,086 - RR42_RS07535 0.31 -0.4 1,611,121 + RR42_RS07535 0.35 -0.1 1,611,122 - RR42_RS07535 0.35 -0.3 1,611,176 + RR42_RS07535 0.41 -0.2 1,611,176 + RR42_RS07535 0.41 +0.7 1,611,176 + RR42_RS07535 0.41 +1.1 1,611,176 + RR42_RS07535 0.41 -3.0 1,611,176 + RR42_RS07535 0.41 -0.2 1,611,177 - RR42_RS07535 0.41 +0.2 1,611,177 - RR42_RS07535 0.41 +0.3 1,611,177 - RR42_RS07535 0.41 -0.0 1,611,239 - RR42_RS07535 0.48 +0.6 1,611,248 - RR42_RS07535 0.49 +0.1 1,611,295 + RR42_RS07535 0.55 +1.6 1,611,296 - RR42_RS07535 0.55 -0.1 1,611,296 - RR42_RS07535 0.55 -1.2 1,611,394 + RR42_RS07535 0.66 +0.0 1,611,583 - RR42_RS07535 0.88 -0.4 1,611,629 - +0.4 1,611,704 - -0.8 1,611,807 + RR42_RS07540 0.11 -0.4 1,611,808 - RR42_RS07540 0.11 -0.4 1,611,906 - RR42_RS07540 0.21 -2.2 1,611,938 + RR42_RS07540 0.24 -0.1 1,612,007 - RR42_RS07540 0.31 -1.0 1,612,085 + RR42_RS07540 0.38 +0.0 1,612,085 + RR42_RS07540 0.38 -0.1 1,612,099 + RR42_RS07540 0.40 +0.4 1,612,124 + RR42_RS07540 0.42 +0.7 1,612,124 + RR42_RS07540 0.42 +0.7 1,612,124 + RR42_RS07540 0.42 -0.5 1,612,124 - RR42_RS07540 0.42 +0.7 1,612,125 - RR42_RS07540 0.42 -0.6 1,612,125 - RR42_RS07540 0.42 +1.1 1,612,125 - RR42_RS07540 0.42 -0.9 1,612,172 + RR42_RS07540 0.47 +0.2 1,612,172 + RR42_RS07540 0.47 +0.3 1,612,172 + RR42_RS07540 0.47 -0.6 1,612,172 + RR42_RS07540 0.47 +0.1 1,612,172 + RR42_RS07540 0.47 +1.0 1,612,172 + RR42_RS07540 0.47 -0.2 1,612,172 + RR42_RS07540 0.47 +0.0 1,612,172 + RR42_RS07540 0.47 -0.2 1,612,173 - RR42_RS07540 0.47 -0.5 1,612,173 - RR42_RS07540 0.47 -0.2 1,612,173 - RR42_RS07540 0.47 +1.0 1,612,173 - RR42_RS07540 0.47 +0.1 1,612,173 - RR42_RS07540 0.47 -0.2 1,612,173 - RR42_RS07540 0.47 -0.1 1,612,173 - RR42_RS07540 0.47 -0.8 1,612,174 + RR42_RS07540 0.47 +0.4 1,612,175 - RR42_RS07540 0.47 +0.5 1,612,175 - RR42_RS07540 0.47 +0.3 1,612,175 - RR42_RS07540 0.47 +0.4 1,612,175 - RR42_RS07540 0.47 +1.5 1,612,175 - RR42_RS07540 0.47 +0.1 1,612,175 - RR42_RS07540 0.47 +0.4 1,612,179 - RR42_RS07540 0.47 -0.4 1,612,179 - RR42_RS07540 0.47 -1.0 1,612,244 + RR42_RS07540 0.54 -0.1 1,612,245 - RR42_RS07540 0.54 -0.1 1,612,294 - RR42_RS07540 0.59 -0.1 1,612,340 - RR42_RS07540 0.63 -2.1 1,612,376 + RR42_RS07540 0.67 +0.7 1,612,376 + RR42_RS07540 0.67 +0.5 1,612,376 + RR42_RS07540 0.67 -0.0 1,612,376 + RR42_RS07540 0.67 -0.2 1,612,376 + RR42_RS07540 0.67 +1.0 1,612,376 + RR42_RS07540 0.67 +0.2 1,612,377 - RR42_RS07540 0.67 +0.6 1,612,377 - RR42_RS07540 0.67 -0.1 1,612,434 - RR42_RS07540 0.72 -0.6 1,612,439 + RR42_RS07540 0.73 -0.4 1,612,439 + RR42_RS07540 0.73 -0.6 1,612,439 + RR42_RS07540 0.73 -0.6 1,612,440 - RR42_RS07540 0.73 -0.3 1,612,440 - RR42_RS07540 0.73 -0.8 1,612,881 + RR42_RS07545 0.20 -0.5 1,612,881 + RR42_RS07545 0.20 -0.0 1,612,881 + RR42_RS07545 0.20 -0.5 1,612,882 - RR42_RS07545 0.20 -1.0 1,612,882 - RR42_RS07545 0.20 -1.6 1,612,882 - RR42_RS07545 0.20 +0.2 1,612,882 - RR42_RS07545 0.20 +0.2 1,612,882 - RR42_RS07545 0.20 +0.3 1,612,882 - RR42_RS07545 0.20 -1.3 1,613,004 + RR42_RS07545 0.34 +0.2 1,613,005 - RR42_RS07545 0.34 -1.5 1,613,005 - RR42_RS07545 0.34 +0.2 1,613,465 - RR42_RS07545 0.88 +0.5 1,613,465 - RR42_RS07545 0.88 +0.3 1,613,545 - -0.4 1,613,607 + -1.4 1,613,608 - -1.2 1,613,608 - +0.2 1,613,711 + RR42_RS07550 0.21 +1.5 1,613,711 + RR42_RS07550 0.21 +0.1 1,613,712 - RR42_RS07550 0.21 +1.8
Or see this region's nucleotide sequence