Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01285 and RR42_RS01290 are separated by 461 nucleotides RR42_RS01290 and RR42_RS01295 are separated by 141 nucleotides
RR42_RS01285: RR42_RS01285 - acyl-CoA dehydrogenase, at 291,749 to 293,410
_RS01285
RR42_RS01290: RR42_RS01290 - thiamine biosynthesis protein ThiC, at 293,872 to 295,758
_RS01290
RR42_RS01295: RR42_RS01295 - cytochrome C biogenesis protein CcdA, at 295,900 to 297,078
_RS01295
Position (kb) 293
294
295
296 Strain fitness (log2 ratio) -2
-1
0
1
2 at 293.197 kb on + strand, within RR42_RS01285 at 293.197 kb on + strand, within RR42_RS01285 at 293.197 kb on + strand, within RR42_RS01285 at 293.198 kb on - strand, within RR42_RS01285 at 293.198 kb on - strand, within RR42_RS01285 at 293.277 kb on - strand at 293.485 kb on + strand at 293.485 kb on + strand at 293.486 kb on - strand at 293.527 kb on - strand at 293.527 kb on - strand at 293.593 kb on + strand at 293.594 kb on - strand at 293.594 kb on - strand at 293.594 kb on - strand at 294.340 kb on + strand, within RR42_RS01290 at 294.340 kb on + strand, within RR42_RS01290 at 294.394 kb on + strand, within RR42_RS01290 at 294.760 kb on + strand, within RR42_RS01290 at 294.856 kb on + strand, within RR42_RS01290 at 294.856 kb on + strand, within RR42_RS01290 at 294.889 kb on + strand, within RR42_RS01290 at 294.889 kb on + strand, within RR42_RS01290 at 295.209 kb on + strand, within RR42_RS01290 at 295.234 kb on + strand, within RR42_RS01290 at 295.234 kb on + strand, within RR42_RS01290 at 295.564 kb on + strand, within RR42_RS01290 at 295.750 kb on + strand at 295.750 kb on + strand at 295.756 kb on + strand at 295.763 kb on + strand at 295.808 kb on + strand at 295.844 kb on + strand at 295.844 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 293,197 + RR42_RS01285 0.87 +0.4 293,197 + RR42_RS01285 0.87 -0.1 293,197 + RR42_RS01285 0.87 +0.7 293,198 - RR42_RS01285 0.87 +0.9 293,198 - RR42_RS01285 0.87 +0.1 293,277 - -0.5 293,485 + +1.9 293,485 + -0.3 293,486 - +0.2 293,527 - -0.2 293,527 - +0.8 293,593 + -0.2 293,594 - -0.2 293,594 - -0.3 293,594 - +0.4 294,340 + RR42_RS01290 0.25 +0.0 294,340 + RR42_RS01290 0.25 +0.3 294,394 + RR42_RS01290 0.28 -0.4 294,760 + RR42_RS01290 0.47 +0.3 294,856 + RR42_RS01290 0.52 -0.5 294,856 + RR42_RS01290 0.52 -0.1 294,889 + RR42_RS01290 0.54 -0.1 294,889 + RR42_RS01290 0.54 -0.6 295,209 + RR42_RS01290 0.71 +0.5 295,234 + RR42_RS01290 0.72 -0.0 295,234 + RR42_RS01290 0.72 -0.8 295,564 + RR42_RS01290 0.90 -0.1 295,750 + -0.9 295,750 + -0.9 295,756 + -1.8 295,763 + +0.6 295,808 + +0.2 295,844 + +0.5 295,844 + +1.2
Or see this region's nucleotide sequence