Experiment: R2A with sodium fluoride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00615 and RR42_RS00620 overlap by 4 nucleotides RR42_RS00620 and RR42_RS00625 are separated by 230 nucleotides RR42_RS00625 and RR42_RS00630 are separated by 177 nucleotides
RR42_RS00615: RR42_RS00615 - recombinase XerC, at 161,286 to 162,410
_RS00615
RR42_RS00620: RR42_RS00620 - XRE family transcriptional regulator, at 162,407 to 163,366
_RS00620
RR42_RS00625: RR42_RS00625 - hypothetical protein, at 163,597 to 163,956
_RS00625
RR42_RS00630: RR42_RS00630 - lipoate--protein ligase, at 164,134 to 164,865
_RS00630
Position (kb) 162
163
164 Strain fitness (log2 ratio) -2
-1
0
1
2 at 161.422 kb on - strand, within RR42_RS00615 at 161.505 kb on - strand, within RR42_RS00615 at 161.772 kb on + strand, within RR42_RS00615 at 161.772 kb on + strand, within RR42_RS00615 at 161.820 kb on + strand, within RR42_RS00615 at 161.839 kb on - strand, within RR42_RS00615 at 162.051 kb on - strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.220 kb on - strand, within RR42_RS00615 at 162.220 kb on - strand, within RR42_RS00615 at 162.255 kb on + strand, within RR42_RS00615 at 162.255 kb on + strand, within RR42_RS00615 at 162.479 kb on + strand at 162.479 kb on + strand at 162.479 kb on + strand at 162.480 kb on - strand at 162.480 kb on - strand at 162.519 kb on + strand, within RR42_RS00620 at 162.519 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 163.413 kb on + strand at 163.444 kb on + strand at 163.445 kb on - strand at 163.445 kb on - strand at 163.445 kb on - strand at 163.490 kb on + strand at 163.509 kb on + strand at 163.742 kb on - strand, within RR42_RS00625 at 164.122 kb on + strand at 164.145 kb on - strand at 164.145 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 100 mM remove 161,422 - RR42_RS00615 0.12 -1.3 161,505 - RR42_RS00615 0.19 -1.5 161,772 + RR42_RS00615 0.43 -1.7 161,772 + RR42_RS00615 0.43 -1.6 161,820 + RR42_RS00615 0.47 -1.1 161,839 - RR42_RS00615 0.49 -1.2 162,051 - RR42_RS00615 0.68 -1.3 162,219 + RR42_RS00615 0.83 -1.9 162,219 + RR42_RS00615 0.83 -0.8 162,219 + RR42_RS00615 0.83 -1.5 162,219 + RR42_RS00615 0.83 +0.2 162,220 - RR42_RS00615 0.83 -1.6 162,220 - RR42_RS00615 0.83 -1.6 162,255 + RR42_RS00615 0.86 +1.3 162,255 + RR42_RS00615 0.86 +0.4 162,479 + -0.3 162,479 + -0.1 162,479 + +0.7 162,480 - -2.1 162,480 - +0.9 162,519 + RR42_RS00620 0.12 +1.4 162,519 + RR42_RS00620 0.12 -0.1 162,538 + RR42_RS00620 0.14 -0.2 162,538 + RR42_RS00620 0.14 -0.6 162,538 + RR42_RS00620 0.14 +1.3 162,538 + RR42_RS00620 0.14 -1.1 162,545 + RR42_RS00620 0.14 -0.2 162,545 + RR42_RS00620 0.14 -1.0 162,545 + RR42_RS00620 0.14 +0.4 162,545 + RR42_RS00620 0.14 -0.5 162,546 - RR42_RS00620 0.14 +0.8 162,546 - RR42_RS00620 0.14 -2.5 162,546 - RR42_RS00620 0.14 +0.2 162,546 - RR42_RS00620 0.14 +0.0 162,588 + RR42_RS00620 0.19 +0.9 162,588 + RR42_RS00620 0.19 -0.1 162,588 + RR42_RS00620 0.19 -0.8 162,849 + RR42_RS00620 0.46 +0.3 162,849 + RR42_RS00620 0.46 +0.3 162,849 + RR42_RS00620 0.46 +0.9 162,849 + RR42_RS00620 0.46 +0.2 162,850 - RR42_RS00620 0.46 -0.4 162,850 - RR42_RS00620 0.46 -0.9 162,850 - RR42_RS00620 0.46 +0.8 162,850 - RR42_RS00620 0.46 -0.8 162,850 - RR42_RS00620 0.46 +1.4 163,413 + -0.3 163,444 + -0.7 163,445 - +1.3 163,445 - -0.7 163,445 - +0.4 163,490 + -0.7 163,509 + +1.0 163,742 - RR42_RS00625 0.40 +0.0 164,122 + +2.3 164,145 - -0.1 164,145 - +0.1
Or see this region's nucleotide sequence