Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_87 and Pf1N1B4_88 are separated by 298 nucleotides Pf1N1B4_88 and Pf1N1B4_89 are separated by 131 nucleotides Pf1N1B4_89 and Pf1N1B4_90 are separated by 245 nucleotides
Pf1N1B4_87 - DNA polymerase II (EC 2.7.7.7), at 89,800 to 92,187
_87
Pf1N1B4_88 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 92,486 to 92,926
_88
Pf1N1B4_89 - Uncharacterized GST-like protein yncG, at 93,058 to 93,678
_89
Pf1N1B4_90 - Putative outer membrane lipoprotein, at 93,924 to 95,018
_90
Position (kb)
93
94 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 92.152 kb on + strand at 92.181 kb on + strand at 92.182 kb on - strand at 92.198 kb on + strand at 92.198 kb on + strand at 92.347 kb on + strand at 92.347 kb on + strand at 92.370 kb on + strand at 92.370 kb on + strand at 92.446 kb on - strand at 92.545 kb on + strand, within Pf1N1B4_88 at 92.545 kb on + strand, within Pf1N1B4_88 at 92.546 kb on - strand, within Pf1N1B4_88 at 92.546 kb on - strand, within Pf1N1B4_88 at 92.546 kb on - strand at 92.546 kb on - strand, within Pf1N1B4_88 at 92.660 kb on + strand, within Pf1N1B4_88 at 92.661 kb on - strand, within Pf1N1B4_88 at 92.909 kb on + strand at 92.910 kb on - strand at 92.910 kb on - strand at 92.910 kb on - strand at 92.910 kb on - strand at 92.991 kb on + strand at 92.995 kb on + strand at 92.995 kb on + strand at 92.995 kb on + strand at 92.996 kb on - strand at 93.008 kb on - strand at 93.227 kb on + strand, within Pf1N1B4_89 at 93.389 kb on + strand, within Pf1N1B4_89 at 93.389 kb on + strand, within Pf1N1B4_89 at 93.389 kb on + strand, within Pf1N1B4_89 at 93.389 kb on + strand at 93.389 kb on + strand, within Pf1N1B4_89 at 93.389 kb on + strand, within Pf1N1B4_89 at 93.389 kb on + strand, within Pf1N1B4_89 at 93.389 kb on + strand, within Pf1N1B4_89 at 93.389 kb on + strand at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.390 kb on - strand, within Pf1N1B4_89 at 93.474 kb on + strand, within Pf1N1B4_89 at 93.475 kb on - strand at 93.475 kb on - strand at 93.674 kb on + strand at 93.674 kb on + strand at 93.674 kb on + strand at 93.674 kb on + strand at 93.674 kb on + strand at 93.674 kb on + strand at 93.675 kb on - strand at 93.675 kb on - strand at 93.675 kb on - strand at 93.675 kb on - strand at 93.682 kb on + strand at 93.682 kb on + strand at 93.682 kb on + strand at 93.682 kb on + strand at 93.683 kb on - strand at 93.683 kb on - strand at 93.709 kb on + strand at 93.736 kb on + strand at 93.750 kb on + strand at 93.750 kb on + strand at 93.750 kb on + strand at 93.751 kb on - strand at 93.760 kb on + strand at 93.830 kb on - strand at 93.830 kb on - strand at 93.875 kb on + strand at 93.876 kb on - strand at 93.944 kb on + strand at 93.945 kb on - strand at 93.945 kb on - strand at 93.945 kb on - strand at 93.945 kb on - strand at 94.050 kb on + strand, within Pf1N1B4_90 at 94.051 kb on - strand, within Pf1N1B4_90 at 94.127 kb on + strand, within Pf1N1B4_90 at 94.128 kb on - strand, within Pf1N1B4_90 at 94.396 kb on + strand, within Pf1N1B4_90 at 94.396 kb on + strand, within Pf1N1B4_90 at 94.397 kb on - strand, within Pf1N1B4_90 at 94.457 kb on - strand, within Pf1N1B4_90 at 94.657 kb on - strand, within Pf1N1B4_90
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 92,152 + -0.3 92,181 + -0.6 92,182 - +0.2 92,198 + +2.6 92,198 + -0.3 92,347 + -0.5 92,347 + -0.8 92,370 + +1.6 92,370 + -0.4 92,446 - -1.6 92,545 + Pf1N1B4_88 0.13 -0.7 92,545 + Pf1N1B4_88 0.13 +2.9 92,546 - Pf1N1B4_88 0.14 -0.6 92,546 - Pf1N1B4_88 0.14 -0.4 92,546 - +0.3 92,546 - Pf1N1B4_88 0.14 -1.8 92,660 + Pf1N1B4_88 0.39 +0.2 92,661 - Pf1N1B4_88 0.40 -0.3 92,909 + +0.1 92,910 - -3.1 92,910 - +1.7 92,910 - +0.7 92,910 - -0.1 92,991 + -0.8 92,995 + -0.8 92,995 + -2.3 92,995 + +0.3 92,996 - -0.0 93,008 - +0.2 93,227 + Pf1N1B4_89 0.27 +0.1 93,389 + Pf1N1B4_89 0.53 +1.3 93,389 + Pf1N1B4_89 0.53 -0.4 93,389 + Pf1N1B4_89 0.53 -1.3 93,389 + -0.3 93,389 + Pf1N1B4_89 0.53 +2.6 93,389 + Pf1N1B4_89 0.53 +0.3 93,389 + Pf1N1B4_89 0.53 +1.0 93,389 + Pf1N1B4_89 0.53 +1.2 93,389 + +0.3 93,390 - Pf1N1B4_89 0.53 +1.7 93,390 - Pf1N1B4_89 0.53 -1.3 93,390 - Pf1N1B4_89 0.53 +0.7 93,390 - Pf1N1B4_89 0.53 +0.6 93,390 - Pf1N1B4_89 0.53 -1.6 93,390 - -1.3 93,390 - Pf1N1B4_89 0.53 +0.7 93,390 - Pf1N1B4_89 0.53 +1.1 93,390 - Pf1N1B4_89 0.53 +2.3 93,390 - Pf1N1B4_89 0.53 +0.3 93,390 - Pf1N1B4_89 0.53 +0.4 93,390 - Pf1N1B4_89 0.53 -0.6 93,390 - Pf1N1B4_89 0.53 +0.3 93,390 - Pf1N1B4_89 0.53 +1.0 93,474 + Pf1N1B4_89 0.67 +1.6 93,475 - +1.3 93,475 - +1.7 93,674 + -1.3 93,674 + -0.8 93,674 + +0.9 93,674 + +1.2 93,674 + +1.3 93,674 + -0.3 93,675 - +0.3 93,675 - -0.7 93,675 - +0.7 93,675 - -0.6 93,682 + -0.8 93,682 + -1.3 93,682 + -2.8 93,682 + -1.8 93,683 - -1.4 93,683 - +0.7 93,709 + -1.8 93,736 + -0.3 93,750 + +1.3 93,750 + +0.7 93,750 + +0.7 93,751 - -0.5 93,760 + +0.9 93,830 - -0.3 93,830 - -0.0 93,875 + -0.3 93,876 - +0.6 93,944 + -0.4 93,945 - -1.3 93,945 - -2.8 93,945 - -0.5 93,945 - -0.3 94,050 + Pf1N1B4_90 0.12 -0.6 94,051 - Pf1N1B4_90 0.12 +0.3 94,127 + Pf1N1B4_90 0.19 -0.6 94,128 - Pf1N1B4_90 0.19 +0.5 94,396 + Pf1N1B4_90 0.43 +0.2 94,396 + Pf1N1B4_90 0.43 -1.8 94,397 - Pf1N1B4_90 0.43 -0.9 94,457 - Pf1N1B4_90 0.49 -0.4 94,657 - Pf1N1B4_90 0.67 -1.3
Or see this region's nucleotide sequence