Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5656 and Pf1N1B4_5657 overlap by 4 nucleotides Pf1N1B4_5657 and Pf1N1B4_5658 are separated by 82 nucleotides Pf1N1B4_5658 and Pf1N1B4_5659 are separated by 183 nucleotides Pf1N1B4_5659 and Pf1N1B4_5660 are separated by 213 nucleotides
Pf1N1B4_5656 - Predicted cobalt transporter CbtA, at 87,675 to 88,382
_5656
Pf1N1B4_5657 - Cobalt-precorrin-3b C17-methyltransferase, at 88,379 to 88,792
_5657
Pf1N1B4_5658 - Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133), at 88,875 to 89,621
_5658
Pf1N1B4_5659 - NfuA Fe-S protein maturation, at 89,805 to 90,389
_5659
Pf1N1B4_5660 - cis/trans isomerase, at 90,603 to 92,888
_5660
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 87.986 kb on + strand at 87.986 kb on + strand, within Pf1N1B4_5656 at 88.372 kb on - strand at 88.884 kb on + strand at 88.885 kb on - strand at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.969 kb on - strand at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.997 kb on - strand, within Pf1N1B4_5658 at 89.131 kb on - strand, within Pf1N1B4_5658 at 89.586 kb on + strand at 89.807 kb on - strand at 89.809 kb on + strand at 89.809 kb on + strand at 89.809 kb on + strand at 89.809 kb on + strand at 89.809 kb on + strand at 89.809 kb on + strand at 89.810 kb on - strand at 89.810 kb on - strand at 89.810 kb on - strand at 89.810 kb on - strand at 89.810 kb on - strand at 89.812 kb on + strand at 89.813 kb on - strand at 89.871 kb on - strand, within Pf1N1B4_5659 at 89.871 kb on - strand, within Pf1N1B4_5659 at 89.871 kb on - strand, within Pf1N1B4_5659 at 89.871 kb on - strand, within Pf1N1B4_5659 at 89.871 kb on - strand, within Pf1N1B4_5659 at 89.871 kb on - strand, within Pf1N1B4_5659 at 89.871 kb on - strand, within Pf1N1B4_5659 at 90.040 kb on + strand, within Pf1N1B4_5659 at 90.040 kb on + strand, within Pf1N1B4_5659 at 90.040 kb on + strand, within Pf1N1B4_5659 at 90.040 kb on + strand, within Pf1N1B4_5659 at 90.040 kb on + strand, within Pf1N1B4_5659 at 90.041 kb on - strand, within Pf1N1B4_5659 at 90.041 kb on - strand, within Pf1N1B4_5659 at 90.041 kb on - strand, within Pf1N1B4_5659 at 90.041 kb on - strand, within Pf1N1B4_5659 at 90.041 kb on - strand, within Pf1N1B4_5659 at 90.041 kb on - strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.043 kb on + strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.044 kb on - strand, within Pf1N1B4_5659 at 90.087 kb on + strand, within Pf1N1B4_5659 at 90.087 kb on + strand, within Pf1N1B4_5659 at 90.088 kb on - strand, within Pf1N1B4_5659 at 90.088 kb on - strand, within Pf1N1B4_5659 at 90.088 kb on - strand, within Pf1N1B4_5659 at 90.088 kb on - strand, within Pf1N1B4_5659 at 90.088 kb on - strand, within Pf1N1B4_5659 at 90.142 kb on + strand, within Pf1N1B4_5659 at 90.190 kb on + strand, within Pf1N1B4_5659 at 90.190 kb on + strand, within Pf1N1B4_5659 at 90.190 kb on + strand, within Pf1N1B4_5659 at 90.190 kb on + strand, within Pf1N1B4_5659 at 90.191 kb on - strand, within Pf1N1B4_5659 at 90.191 kb on - strand, within Pf1N1B4_5659 at 90.191 kb on - strand, within Pf1N1B4_5659 at 90.191 kb on - strand at 90.191 kb on - strand, within Pf1N1B4_5659 at 90.191 kb on - strand, within Pf1N1B4_5659 at 90.253 kb on + strand, within Pf1N1B4_5659 at 90.254 kb on - strand, within Pf1N1B4_5659 at 90.274 kb on + strand, within Pf1N1B4_5659 at 90.296 kb on + strand, within Pf1N1B4_5659 at 90.296 kb on + strand, within Pf1N1B4_5659 at 90.296 kb on + strand, within Pf1N1B4_5659 at 90.296 kb on + strand, within Pf1N1B4_5659 at 90.297 kb on - strand, within Pf1N1B4_5659 at 90.352 kb on + strand at 90.353 kb on - strand at 90.353 kb on - strand at 90.353 kb on - strand at 90.377 kb on + strand at 90.377 kb on + strand at 90.377 kb on + strand at 90.377 kb on + strand at 90.378 kb on - strand at 90.378 kb on - strand at 90.378 kb on - strand at 90.378 kb on - strand at 90.378 kb on - strand at 90.378 kb on - strand at 90.378 kb on - strand at 90.378 kb on - strand at 90.380 kb on + strand at 90.380 kb on + strand at 90.381 kb on - strand at 90.419 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 87,986 + -1.5 87,986 + Pf1N1B4_5656 0.44 -1.6 88,372 - -0.7 88,884 + +0.0 88,885 - -2.0 88,968 + Pf1N1B4_5658 0.12 -0.5 88,968 + Pf1N1B4_5658 0.12 +1.1 88,968 + Pf1N1B4_5658 0.12 +0.5 88,968 + Pf1N1B4_5658 0.12 -0.9 88,968 + Pf1N1B4_5658 0.12 -1.2 88,969 - -1.5 88,969 - Pf1N1B4_5658 0.13 -0.1 88,969 - Pf1N1B4_5658 0.13 -1.0 88,969 - Pf1N1B4_5658 0.13 -1.6 88,969 - Pf1N1B4_5658 0.13 -2.8 88,997 - Pf1N1B4_5658 0.16 -1.2 89,131 - Pf1N1B4_5658 0.34 -1.0 89,586 + -1.3 89,807 - -0.5 89,809 + -1.5 89,809 + -2.0 89,809 + +1.1 89,809 + +0.4 89,809 + +0.1 89,809 + -1.2 89,810 - +0.5 89,810 - +0.9 89,810 - +0.3 89,810 - +1.7 89,810 - -0.1 89,812 + +0.7 89,813 - +0.1 89,871 - Pf1N1B4_5659 0.11 -0.0 89,871 - Pf1N1B4_5659 0.11 -0.8 89,871 - Pf1N1B4_5659 0.11 +0.1 89,871 - Pf1N1B4_5659 0.11 -1.5 89,871 - Pf1N1B4_5659 0.11 -0.8 89,871 - Pf1N1B4_5659 0.11 +0.7 89,871 - Pf1N1B4_5659 0.11 +1.5 90,040 + Pf1N1B4_5659 0.40 -0.5 90,040 + Pf1N1B4_5659 0.40 +0.4 90,040 + Pf1N1B4_5659 0.40 -0.2 90,040 + Pf1N1B4_5659 0.40 +0.1 90,040 + Pf1N1B4_5659 0.40 -0.1 90,041 - Pf1N1B4_5659 0.40 +0.1 90,041 - Pf1N1B4_5659 0.40 -1.5 90,041 - Pf1N1B4_5659 0.40 -0.5 90,041 - Pf1N1B4_5659 0.40 +0.5 90,041 - Pf1N1B4_5659 0.40 -0.7 90,041 - Pf1N1B4_5659 0.40 -1.8 90,043 + Pf1N1B4_5659 0.41 -0.8 90,043 + Pf1N1B4_5659 0.41 +0.1 90,043 + Pf1N1B4_5659 0.41 +1.9 90,043 + Pf1N1B4_5659 0.41 -1.5 90,043 + Pf1N1B4_5659 0.41 -0.9 90,043 + Pf1N1B4_5659 0.41 -0.3 90,043 + Pf1N1B4_5659 0.41 -0.8 90,043 + Pf1N1B4_5659 0.41 -0.5 90,043 + Pf1N1B4_5659 0.41 +0.4 90,044 - Pf1N1B4_5659 0.41 +0.1 90,044 - Pf1N1B4_5659 0.41 +0.3 90,044 - Pf1N1B4_5659 0.41 -0.3 90,044 - Pf1N1B4_5659 0.41 -0.4 90,044 - Pf1N1B4_5659 0.41 +0.9 90,044 - Pf1N1B4_5659 0.41 -0.2 90,044 - Pf1N1B4_5659 0.41 -0.1 90,044 - Pf1N1B4_5659 0.41 -0.1 90,044 - Pf1N1B4_5659 0.41 -0.8 90,044 - Pf1N1B4_5659 0.41 +0.1 90,044 - Pf1N1B4_5659 0.41 -0.5 90,044 - Pf1N1B4_5659 0.41 -0.5 90,044 - Pf1N1B4_5659 0.41 -1.8 90,044 - Pf1N1B4_5659 0.41 -1.0 90,087 + Pf1N1B4_5659 0.48 -0.0 90,087 + Pf1N1B4_5659 0.48 +1.5 90,088 - Pf1N1B4_5659 0.48 -1.0 90,088 - Pf1N1B4_5659 0.48 -0.2 90,088 - Pf1N1B4_5659 0.48 -0.2 90,088 - Pf1N1B4_5659 0.48 -0.1 90,088 - Pf1N1B4_5659 0.48 +1.5 90,142 + Pf1N1B4_5659 0.58 +0.1 90,190 + Pf1N1B4_5659 0.66 -0.5 90,190 + Pf1N1B4_5659 0.66 +0.1 90,190 + Pf1N1B4_5659 0.66 +2.1 90,190 + Pf1N1B4_5659 0.66 -0.2 90,191 - Pf1N1B4_5659 0.66 -1.1 90,191 - Pf1N1B4_5659 0.66 -0.7 90,191 - Pf1N1B4_5659 0.66 -0.9 90,191 - -1.5 90,191 - Pf1N1B4_5659 0.66 -0.8 90,191 - Pf1N1B4_5659 0.66 -0.7 90,253 + Pf1N1B4_5659 0.77 -1.0 90,254 - Pf1N1B4_5659 0.77 -0.5 90,274 + Pf1N1B4_5659 0.80 +0.0 90,296 + Pf1N1B4_5659 0.84 +0.1 90,296 + Pf1N1B4_5659 0.84 -0.2 90,296 + Pf1N1B4_5659 0.84 +0.1 90,296 + Pf1N1B4_5659 0.84 -0.5 90,297 - Pf1N1B4_5659 0.84 +1.3 90,352 + +0.2 90,353 - -0.9 90,353 - -1.3 90,353 - +0.2 90,377 + -0.5 90,377 + -0.2 90,377 + -0.5 90,377 + +0.5 90,378 - -2.0 90,378 - -0.2 90,378 - +0.4 90,378 - +0.4 90,378 - -0.1 90,378 - -0.9 90,378 - +0.5 90,378 - +0.1 90,380 + -1.8 90,380 + +0.1 90,381 - +0.5 90,419 + -0.1
Or see this region's nucleotide sequence