Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5325 and Pf1N1B4_5326 are separated by 183 nucleotides Pf1N1B4_5326 and Pf1N1B4_5327 are separated by 90 nucleotides Pf1N1B4_5327 and Pf1N1B4_5328 are separated by 39 nucleotides Pf1N1B4_5328 and Pf1N1B4_5329 are separated by 219 nucleotides
Pf1N1B4_5325 - hypothetical protein, at 74,720 to 75,979
_5325
Pf1N1B4_5326 - hypothetical protein, at 76,163 to 76,324
_5326
Pf1N1B4_5327 - hypothetical protein, at 76,415 to 76,909
_5327
Pf1N1B4_5328 - hypothetical protein, at 76,949 to 77,134
_5328
Pf1N1B4_5329 - hypothetical protein, at 77,354 to 77,896
_5329
Position (kb)
76
77 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 75.437 kb on - strand, within Pf1N1B4_5325 at 75.446 kb on + strand, within Pf1N1B4_5325 at 75.447 kb on - strand, within Pf1N1B4_5325 at 75.449 kb on + strand, within Pf1N1B4_5325 at 75.458 kb on - strand, within Pf1N1B4_5325 at 75.496 kb on + strand at 75.497 kb on - strand, within Pf1N1B4_5325 at 75.568 kb on + strand at 75.690 kb on + strand, within Pf1N1B4_5325 at 75.690 kb on + strand, within Pf1N1B4_5325 at 75.800 kb on + strand, within Pf1N1B4_5325 at 75.801 kb on - strand, within Pf1N1B4_5325 at 75.827 kb on + strand, within Pf1N1B4_5325 at 75.828 kb on - strand, within Pf1N1B4_5325 at 75.828 kb on - strand, within Pf1N1B4_5325 at 75.859 kb on + strand at 75.859 kb on + strand at 75.860 kb on - strand at 75.923 kb on - strand at 75.924 kb on + strand at 75.924 kb on + strand at 75.924 kb on + strand at 75.924 kb on + strand at 75.924 kb on + strand at 75.924 kb on + strand at 75.925 kb on - strand at 75.925 kb on - strand at 75.949 kb on + strand at 75.950 kb on - strand at 75.979 kb on + strand at 75.986 kb on + strand at 75.986 kb on + strand at 76.001 kb on - strand at 76.001 kb on - strand at 76.015 kb on - strand at 76.040 kb on + strand at 76.040 kb on + strand at 76.040 kb on + strand at 76.044 kb on + strand at 76.044 kb on + strand at 76.044 kb on + strand at 76.044 kb on + strand at 76.427 kb on - strand at 76.427 kb on - strand at 76.427 kb on - strand at 76.560 kb on + strand, within Pf1N1B4_5327 at 76.561 kb on - strand, within Pf1N1B4_5327 at 76.561 kb on - strand, within Pf1N1B4_5327 at 76.561 kb on - strand, within Pf1N1B4_5327 at 76.648 kb on - strand, within Pf1N1B4_5327 at 76.732 kb on - strand, within Pf1N1B4_5327 at 76.732 kb on - strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.782 kb on + strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.783 kb on - strand, within Pf1N1B4_5327 at 76.830 kb on + strand, within Pf1N1B4_5327 at 76.830 kb on + strand, within Pf1N1B4_5327 at 76.830 kb on + strand, within Pf1N1B4_5327 at 76.830 kb on + strand, within Pf1N1B4_5327 at 76.831 kb on - strand, within Pf1N1B4_5327 at 76.876 kb on + strand at 76.877 kb on - strand at 76.946 kb on - strand at 76.950 kb on + strand at 76.950 kb on + strand at 76.950 kb on + strand at 76.951 kb on - strand at 76.951 kb on - strand at 76.954 kb on + strand at 76.954 kb on + strand at 76.955 kb on - strand at 76.955 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.204 kb on - strand at 77.249 kb on + strand at 77.249 kb on + strand at 77.254 kb on + strand at 77.255 kb on - strand at 77.255 kb on - strand at 77.336 kb on + strand at 77.336 kb on + strand at 77.337 kb on - strand at 77.362 kb on + strand at 77.371 kb on + strand at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.411 kb on - strand, within Pf1N1B4_5329 at 77.686 kb on + strand, within Pf1N1B4_5329 at 77.752 kb on - strand, within Pf1N1B4_5329 at 77.781 kb on + strand, within Pf1N1B4_5329 at 77.781 kb on + strand at 77.782 kb on - strand, within Pf1N1B4_5329 at 77.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 75,437 - Pf1N1B4_5325 0.57 +1.1 75,446 + Pf1N1B4_5325 0.58 -0.4 75,447 - Pf1N1B4_5325 0.58 -0.3 75,449 + Pf1N1B4_5325 0.58 -1.5 75,458 - Pf1N1B4_5325 0.59 +0.9 75,496 + +1.1 75,497 - Pf1N1B4_5325 0.62 -0.0 75,568 + -0.5 75,690 + Pf1N1B4_5325 0.77 -1.5 75,690 + Pf1N1B4_5325 0.77 +0.1 75,800 + Pf1N1B4_5325 0.86 -0.0 75,801 - Pf1N1B4_5325 0.86 +0.1 75,827 + Pf1N1B4_5325 0.88 +0.4 75,828 - Pf1N1B4_5325 0.88 +1.4 75,828 - Pf1N1B4_5325 0.88 +0.1 75,859 + -0.3 75,859 + +1.1 75,860 - -0.6 75,923 - +0.6 75,924 + +1.9 75,924 + +0.1 75,924 + -1.5 75,924 + -1.1 75,924 + -1.3 75,924 + +1.0 75,925 - +1.1 75,925 - -1.2 75,949 + +1.1 75,950 - +1.1 75,979 + -0.9 75,986 + -0.3 75,986 + +1.1 76,001 - +1.1 76,001 - +0.8 76,015 - +0.8 76,040 + -0.1 76,040 + +1.3 76,040 + -1.2 76,044 + +3.2 76,044 + +0.6 76,044 + +0.1 76,044 + +1.6 76,427 - +0.6 76,427 - +0.1 76,427 - +1.1 76,560 + Pf1N1B4_5327 0.29 -0.9 76,561 - Pf1N1B4_5327 0.29 +1.6 76,561 - Pf1N1B4_5327 0.29 +0.6 76,561 - Pf1N1B4_5327 0.29 +0.2 76,648 - Pf1N1B4_5327 0.47 -2.7 76,732 - Pf1N1B4_5327 0.64 -0.4 76,732 - Pf1N1B4_5327 0.64 -1.5 76,782 + Pf1N1B4_5327 0.74 +2.1 76,782 + Pf1N1B4_5327 0.74 +0.3 76,782 + Pf1N1B4_5327 0.74 +1.4 76,782 + Pf1N1B4_5327 0.74 -0.9 76,782 + Pf1N1B4_5327 0.74 -2.3 76,782 + Pf1N1B4_5327 0.74 -0.5 76,782 + Pf1N1B4_5327 0.74 +0.6 76,782 + Pf1N1B4_5327 0.74 +1.9 76,782 + Pf1N1B4_5327 0.74 +0.1 76,782 + Pf1N1B4_5327 0.74 -0.9 76,782 + Pf1N1B4_5327 0.74 +1.9 76,782 + Pf1N1B4_5327 0.74 +0.1 76,783 - Pf1N1B4_5327 0.74 +1.1 76,783 - Pf1N1B4_5327 0.74 -1.2 76,783 - Pf1N1B4_5327 0.74 +0.7 76,783 - Pf1N1B4_5327 0.74 +0.6 76,783 - Pf1N1B4_5327 0.74 +0.6 76,783 - Pf1N1B4_5327 0.74 -0.6 76,783 - Pf1N1B4_5327 0.74 +0.9 76,783 - Pf1N1B4_5327 0.74 -2.3 76,783 - Pf1N1B4_5327 0.74 +1.9 76,830 + Pf1N1B4_5327 0.84 +1.5 76,830 + Pf1N1B4_5327 0.84 +1.3 76,830 + Pf1N1B4_5327 0.84 +1.1 76,830 + Pf1N1B4_5327 0.84 +0.1 76,831 - Pf1N1B4_5327 0.84 +0.1 76,876 + -0.1 76,877 - +0.5 76,946 - -0.6 76,950 + -0.5 76,950 + +0.1 76,950 + -0.9 76,951 - +0.3 76,951 - -1.5 76,954 + -1.1 76,954 + +2.1 76,955 - -0.5 76,955 - +0.6 77,161 - -0.9 77,161 - +0.1 77,161 - +0.4 77,161 - +2.6 77,204 - -0.2 77,249 + -1.5 77,249 + -0.5 77,254 + -1.5 77,255 - +0.1 77,255 - +0.1 77,336 + -0.4 77,336 + +0.5 77,337 - +0.8 77,362 + -1.7 77,371 + -1.3 77,410 + Pf1N1B4_5329 0.10 +2.9 77,410 + Pf1N1B4_5329 0.10 +1.1 77,410 + Pf1N1B4_5329 0.10 -1.7 77,410 + Pf1N1B4_5329 0.10 +0.5 77,411 - Pf1N1B4_5329 0.10 +0.6 77,686 + Pf1N1B4_5329 0.61 -1.7 77,752 - Pf1N1B4_5329 0.73 +0.6 77,781 + Pf1N1B4_5329 0.79 -0.1 77,781 + +0.5 77,782 - Pf1N1B4_5329 0.79 +0.4 77,847 + +0.1
Or see this region's nucleotide sequence