Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4623 and Pf1N1B4_4624 are separated by 10 nucleotides Pf1N1B4_4624 and Pf1N1B4_4625 are separated by 199 nucleotides Pf1N1B4_4625 and Pf1N1B4_4626 overlap by 14 nucleotides
Pf1N1B4_4623 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data) , at 5,099,613 to 5,100,605
_4623
Pf1N1B4_4624 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26), at 5,100,616 to 5,102,196
_4624
Pf1N1B4_4625 - FIG00955242: hypothetical protein, at 5,102,396 to 5,102,692
_4625
Pf1N1B4_4626 - 3-carboxymuconate cyclase, at 5,102,679 to 5,103,896
_4626
Position (kb)
5100
5101
5102
5103 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5099.793 kb on + strand at 5100.030 kb on + strand, within Pf1N1B4_4623 at 5100.031 kb on - strand, within Pf1N1B4_4623 at 5100.031 kb on - strand, within Pf1N1B4_4623 at 5100.130 kb on - strand, within Pf1N1B4_4623 at 5100.217 kb on - strand, within Pf1N1B4_4623 at 5100.217 kb on - strand at 5100.223 kb on - strand, within Pf1N1B4_4623 at 5100.255 kb on + strand, within Pf1N1B4_4623 at 5100.411 kb on + strand, within Pf1N1B4_4623 at 5100.411 kb on + strand, within Pf1N1B4_4623 at 5100.640 kb on + strand at 5100.641 kb on - strand at 5100.641 kb on - strand at 5100.641 kb on - strand at 5100.912 kb on + strand, within Pf1N1B4_4624 at 5100.913 kb on - strand, within Pf1N1B4_4624 at 5101.020 kb on + strand, within Pf1N1B4_4624 at 5101.051 kb on + strand, within Pf1N1B4_4624 at 5101.064 kb on + strand, within Pf1N1B4_4624 at 5101.642 kb on + strand, within Pf1N1B4_4624 at 5101.642 kb on + strand, within Pf1N1B4_4624 at 5101.776 kb on - strand, within Pf1N1B4_4624 at 5102.140 kb on - strand at 5102.361 kb on - strand at 5102.361 kb on - strand at 5102.361 kb on - strand at 5102.782 kb on - strand at 5103.094 kb on - strand, within Pf1N1B4_4626 at 5103.094 kb on - strand at 5103.094 kb on - strand at 5103.157 kb on + strand at 5103.158 kb on - strand, within Pf1N1B4_4626
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 5,099,793 + +0.4 5,100,030 + Pf1N1B4_4623 0.42 +0.8 5,100,031 - Pf1N1B4_4623 0.42 -0.6 5,100,031 - Pf1N1B4_4623 0.42 -3.2 5,100,130 - Pf1N1B4_4623 0.52 +1.3 5,100,217 - Pf1N1B4_4623 0.61 -0.5 5,100,217 - -0.6 5,100,223 - Pf1N1B4_4623 0.61 -1.1 5,100,255 + Pf1N1B4_4623 0.65 -0.2 5,100,411 + Pf1N1B4_4623 0.80 -0.1 5,100,411 + Pf1N1B4_4623 0.80 +0.4 5,100,640 + -0.6 5,100,641 - -2.8 5,100,641 - +1.0 5,100,641 - -1.9 5,100,912 + Pf1N1B4_4624 0.19 -0.2 5,100,913 - Pf1N1B4_4624 0.19 -1.2 5,101,020 + Pf1N1B4_4624 0.26 -1.6 5,101,051 + Pf1N1B4_4624 0.28 -1.2 5,101,064 + Pf1N1B4_4624 0.28 -1.0 5,101,642 + Pf1N1B4_4624 0.65 +0.4 5,101,642 + Pf1N1B4_4624 0.65 -0.9 5,101,776 - Pf1N1B4_4624 0.73 -1.0 5,102,140 - -0.2 5,102,361 - +0.4 5,102,361 - -0.6 5,102,361 - -2.2 5,102,782 - -0.2 5,103,094 - Pf1N1B4_4626 0.34 -0.3 5,103,094 - -1.6 5,103,094 - -0.6 5,103,157 + -2.6 5,103,158 - Pf1N1B4_4626 0.39 -0.2
Or see this region's nucleotide sequence