Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4093 and Pf1N1B4_4094 are separated by 225 nucleotides Pf1N1B4_4094 and Pf1N1B4_4095 are separated by 10 nucleotides Pf1N1B4_4095 and Pf1N1B4_4096 are separated by 201 nucleotides
Pf1N1B4_4093 - Alkyl hydroperoxide reductase subunit C-like protein, at 4,542,334 to 4,542,987
_4093
Pf1N1B4_4094 - Uncharacterized conserved protein, at 4,543,213 to 4,543,710
_4094
Pf1N1B4_4095 - Transcriptional regulator, LacI family, at 4,543,721 to 4,544,722
_4095
Pf1N1B4_4096 - Nucleoside-diphosphate-sugar epimerases, at 4,544,924 to 4,546,090
_4096
Position (kb) 4543
4544
4545 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.929 kb on - strand at 4542.958 kb on + strand at 4542.959 kb on - strand at 4543.021 kb on + strand at 4543.167 kb on + strand at 4543.167 kb on + strand at 4543.176 kb on - strand at 4543.176 kb on - strand at 4543.380 kb on - strand, within Pf1N1B4_4094 at 4543.445 kb on - strand, within Pf1N1B4_4094 at 4543.536 kb on - strand, within Pf1N1B4_4094 at 4543.928 kb on - strand, within Pf1N1B4_4095 at 4544.388 kb on + strand, within Pf1N1B4_4095 at 4544.639 kb on + strand at 4544.751 kb on + strand at 4544.751 kb on + strand at 4544.751 kb on + strand at 4544.752 kb on - strand at 4544.752 kb on - strand at 4544.752 kb on - strand at 4544.834 kb on - strand at 4544.946 kb on + strand at 4544.947 kb on - strand at 4545.113 kb on + strand, within Pf1N1B4_4096 at 4545.205 kb on - strand at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.348 kb on - strand, within Pf1N1B4_4096 at 4545.348 kb on - strand, within Pf1N1B4_4096 at 4545.382 kb on + strand, within Pf1N1B4_4096 at 4545.382 kb on + strand, within Pf1N1B4_4096 at 4545.431 kb on + strand, within Pf1N1B4_4096
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 4,542,770 - Pf1N1B4_4093 0.67 -1.0 4,542,770 - Pf1N1B4_4093 0.67 +1.6 4,542,929 - -0.4 4,542,958 + -0.4 4,542,959 - -2.4 4,543,021 + -2.4 4,543,167 + +0.6 4,543,167 + -3.0 4,543,176 - -1.6 4,543,176 - -0.4 4,543,380 - Pf1N1B4_4094 0.34 +1.4 4,543,445 - Pf1N1B4_4094 0.47 +1.9 4,543,536 - Pf1N1B4_4094 0.65 -0.3 4,543,928 - Pf1N1B4_4095 0.21 -1.4 4,544,388 + Pf1N1B4_4095 0.67 -0.7 4,544,639 + +1.6 4,544,751 + -2.4 4,544,751 + +0.6 4,544,751 + -0.4 4,544,752 - +1.9 4,544,752 - +1.6 4,544,752 - -0.5 4,544,834 - -2.0 4,544,946 + +1.6 4,544,947 - -0.1 4,545,113 + Pf1N1B4_4096 0.16 -0.4 4,545,205 - -0.4 4,545,347 + Pf1N1B4_4096 0.36 +0.4 4,545,347 + Pf1N1B4_4096 0.36 +0.2 4,545,347 + Pf1N1B4_4096 0.36 -0.4 4,545,348 - Pf1N1B4_4096 0.36 +1.2 4,545,348 - Pf1N1B4_4096 0.36 -1.0 4,545,382 + Pf1N1B4_4096 0.39 -0.4 4,545,382 + Pf1N1B4_4096 0.39 +1.5 4,545,431 + Pf1N1B4_4096 0.43 +0.3
Or see this region's nucleotide sequence