Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3993 and Pf1N1B4_3994 are separated by 142 nucleotides Pf1N1B4_3994 and Pf1N1B4_3995 are separated by 196 nucleotides
Pf1N1B4_3993 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 4,437,190 to 4,438,419
_3993
Pf1N1B4_3994 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 4,438,562 to 4,439,908
_3994
Pf1N1B4_3995 - Exodeoxyribonuclease III (EC 3.1.11.2), at 4,440,105 to 4,440,917
_3995
Position (kb)
4438
4439
4440 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4438.147 kb on + strand, within Pf1N1B4_3993 at 4438.148 kb on - strand, within Pf1N1B4_3993 at 4438.294 kb on + strand at 4438.294 kb on + strand at 4438.294 kb on + strand, within Pf1N1B4_3993 at 4438.294 kb on + strand, within Pf1N1B4_3993 at 4438.294 kb on + strand, within Pf1N1B4_3993 at 4438.295 kb on - strand, within Pf1N1B4_3993 at 4438.295 kb on - strand, within Pf1N1B4_3993 at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.300 kb on + strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.301 kb on - strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.384 kb on + strand at 4438.385 kb on - strand at 4438.385 kb on - strand at 4438.546 kb on + strand at 4438.547 kb on - strand at 4438.547 kb on - strand at 4438.555 kb on - strand at 4438.555 kb on - strand at 4438.555 kb on - strand at 4438.555 kb on - strand at 4438.566 kb on + strand at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.802 kb on + strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.803 kb on - strand, within Pf1N1B4_3994 at 4438.805 kb on - strand, within Pf1N1B4_3994 at 4438.805 kb on - strand, within Pf1N1B4_3994 at 4438.805 kb on - strand, within Pf1N1B4_3994 at 4438.805 kb on - strand, within Pf1N1B4_3994 at 4438.806 kb on + strand, within Pf1N1B4_3994 at 4438.806 kb on + strand at 4438.806 kb on + strand, within Pf1N1B4_3994 at 4438.807 kb on - strand, within Pf1N1B4_3994 at 4438.807 kb on - strand at 4438.807 kb on - strand, within Pf1N1B4_3994 at 4438.807 kb on - strand, within Pf1N1B4_3994 at 4439.070 kb on + strand, within Pf1N1B4_3994 at 4439.071 kb on - strand, within Pf1N1B4_3994 at 4439.184 kb on + strand, within Pf1N1B4_3994 at 4439.184 kb on + strand at 4439.184 kb on + strand, within Pf1N1B4_3994 at 4439.185 kb on - strand, within Pf1N1B4_3994 at 4439.185 kb on - strand, within Pf1N1B4_3994 at 4439.185 kb on - strand, within Pf1N1B4_3994 at 4439.322 kb on + strand, within Pf1N1B4_3994 at 4439.322 kb on + strand at 4439.349 kb on + strand, within Pf1N1B4_3994 at 4439.349 kb on + strand, within Pf1N1B4_3994 at 4439.349 kb on + strand, within Pf1N1B4_3994 at 4439.349 kb on + strand, within Pf1N1B4_3994 at 4439.350 kb on - strand, within Pf1N1B4_3994 at 4439.919 kb on - strand at 4439.919 kb on - strand at 4439.919 kb on - strand at 4439.919 kb on - strand at 4439.919 kb on - strand at 4439.947 kb on + strand at 4439.947 kb on + strand at 4439.947 kb on + strand at 4439.948 kb on - strand at 4440.167 kb on - strand at 4440.688 kb on + strand, within Pf1N1B4_3995 at 4440.688 kb on + strand, within Pf1N1B4_3995 at 4440.689 kb on - strand, within Pf1N1B4_3995 at 4440.843 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 4,438,147 + Pf1N1B4_3993 0.78 -0.6 4,438,148 - Pf1N1B4_3993 0.78 -0.3 4,438,294 + -0.3 4,438,294 + +0.3 4,438,294 + Pf1N1B4_3993 0.90 -0.4 4,438,294 + Pf1N1B4_3993 0.90 +2.3 4,438,294 + Pf1N1B4_3993 0.90 -1.5 4,438,295 - Pf1N1B4_3993 0.90 -1.9 4,438,295 - Pf1N1B4_3993 0.90 -0.3 4,438,300 + -0.3 4,438,300 + +1.0 4,438,300 + +0.3 4,438,300 + -0.1 4,438,300 + -0.3 4,438,300 + -0.3 4,438,300 + -0.7 4,438,300 + +0.5 4,438,300 + -0.7 4,438,300 + -0.3 4,438,300 + -0.3 4,438,300 + -1.3 4,438,300 + -1.3 4,438,300 + +0.7 4,438,300 + -0.3 4,438,300 + -1.0 4,438,301 - -0.7 4,438,301 - +0.1 4,438,301 - +0.1 4,438,301 - +0.7 4,438,301 - +1.3 4,438,301 - -0.3 4,438,301 - +1.3 4,438,301 - -0.6 4,438,384 + -1.3 4,438,384 + -0.3 4,438,384 + +2.7 4,438,384 + -0.3 4,438,384 + +0.7 4,438,384 + -0.6 4,438,384 + +1.0 4,438,384 + +2.0 4,438,384 + -0.7 4,438,384 + -0.3 4,438,384 + +0.3 4,438,385 - +0.7 4,438,385 - -2.3 4,438,546 + +0.3 4,438,547 - +0.9 4,438,547 - +0.7 4,438,555 - +0.3 4,438,555 - -1.3 4,438,555 - -2.3 4,438,555 - -2.3 4,438,566 + -0.6 4,438,802 + Pf1N1B4_3994 0.18 -0.8 4,438,802 + Pf1N1B4_3994 0.18 -0.3 4,438,802 + Pf1N1B4_3994 0.18 +0.0 4,438,802 + Pf1N1B4_3994 0.18 -0.3 4,438,802 + Pf1N1B4_3994 0.18 +0.1 4,438,802 + Pf1N1B4_3994 0.18 -0.3 4,438,802 + Pf1N1B4_3994 0.18 -0.3 4,438,802 + Pf1N1B4_3994 0.18 -0.9 4,438,802 + Pf1N1B4_3994 0.18 -1.3 4,438,802 + Pf1N1B4_3994 0.18 +0.8 4,438,802 + Pf1N1B4_3994 0.18 +0.3 4,438,802 + Pf1N1B4_3994 0.18 -0.9 4,438,803 - Pf1N1B4_3994 0.18 -1.1 4,438,803 - Pf1N1B4_3994 0.18 +0.7 4,438,803 - Pf1N1B4_3994 0.18 -1.9 4,438,803 - Pf1N1B4_3994 0.18 +0.7 4,438,803 - Pf1N1B4_3994 0.18 -0.1 4,438,803 - Pf1N1B4_3994 0.18 +0.0 4,438,803 - Pf1N1B4_3994 0.18 +0.0 4,438,803 - Pf1N1B4_3994 0.18 +1.3 4,438,805 - Pf1N1B4_3994 0.18 +1.3 4,438,805 - Pf1N1B4_3994 0.18 +0.7 4,438,805 - Pf1N1B4_3994 0.18 +2.3 4,438,805 - Pf1N1B4_3994 0.18 +0.3 4,438,806 + Pf1N1B4_3994 0.18 -0.7 4,438,806 + -1.3 4,438,806 + Pf1N1B4_3994 0.18 -1.9 4,438,807 - Pf1N1B4_3994 0.18 -0.6 4,438,807 - +0.7 4,438,807 - Pf1N1B4_3994 0.18 +2.9 4,438,807 - Pf1N1B4_3994 0.18 -1.9 4,439,070 + Pf1N1B4_3994 0.38 -0.3 4,439,071 - Pf1N1B4_3994 0.38 -0.9 4,439,184 + Pf1N1B4_3994 0.46 +0.7 4,439,184 + +0.3 4,439,184 + Pf1N1B4_3994 0.46 -0.3 4,439,185 - Pf1N1B4_3994 0.46 -0.3 4,439,185 - Pf1N1B4_3994 0.46 +0.0 4,439,185 - Pf1N1B4_3994 0.46 -0.9 4,439,322 + Pf1N1B4_3994 0.56 +1.1 4,439,322 + +0.7 4,439,349 + Pf1N1B4_3994 0.58 -1.9 4,439,349 + Pf1N1B4_3994 0.58 +1.7 4,439,349 + Pf1N1B4_3994 0.58 -0.2 4,439,349 + Pf1N1B4_3994 0.58 -0.3 4,439,350 - Pf1N1B4_3994 0.59 -0.3 4,439,919 - +0.7 4,439,919 - +2.0 4,439,919 - -1.3 4,439,919 - -0.3 4,439,919 - -0.3 4,439,947 + +0.7 4,439,947 + -0.3 4,439,947 + -0.1 4,439,948 - -0.6 4,440,167 - -1.3 4,440,688 + Pf1N1B4_3995 0.72 +0.1 4,440,688 + Pf1N1B4_3995 0.72 +1.6 4,440,689 - Pf1N1B4_3995 0.72 +0.4 4,440,843 - -0.8
Or see this region's nucleotide sequence