Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3919 and Pf1N1B4_3920 are separated by 42 nucleotides Pf1N1B4_3920 and Pf1N1B4_3921 are separated by 14 nucleotides Pf1N1B4_3921 and Pf1N1B4_3922 are separated by 160 nucleotides
Pf1N1B4_3919 - Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1), at 4,357,535 to 4,358,929
_3919
Pf1N1B4_3920 - Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46), at 4,358,972 to 4,359,694
_3920
Pf1N1B4_3921 - FIG00953243: hypothetical protein, at 4,359,709 to 4,360,290
_3921
Pf1N1B4_3922 - Lipoprotein releasing system transmembrane protein LolC, at 4,360,451 to 4,361,635
_3922
Position (kb)
4358
4359
4360 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4358.021 kb on + strand at 4358.446 kb on + strand, within Pf1N1B4_3919 at 4358.579 kb on + strand, within Pf1N1B4_3919 at 4358.579 kb on + strand, within Pf1N1B4_3919 at 4358.580 kb on - strand at 4358.843 kb on + strand at 4359.016 kb on + strand at 4359.169 kb on - strand, within Pf1N1B4_3920 at 4359.169 kb on - strand, within Pf1N1B4_3920 at 4359.204 kb on + strand, within Pf1N1B4_3920 at 4359.474 kb on + strand, within Pf1N1B4_3920 at 4359.496 kb on + strand, within Pf1N1B4_3920 at 4359.507 kb on - strand, within Pf1N1B4_3920 at 4359.507 kb on - strand, within Pf1N1B4_3920 at 4359.605 kb on + strand, within Pf1N1B4_3920 at 4359.681 kb on + strand at 4359.682 kb on - strand at 4359.686 kb on - strand at 4359.705 kb on + strand at 4359.705 kb on + strand at 4359.706 kb on - strand at 4359.710 kb on + strand at 4359.774 kb on - strand at 4359.833 kb on + strand, within Pf1N1B4_3921 at 4360.279 kb on + strand at 4360.279 kb on + strand at 4360.279 kb on + strand at 4360.279 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 4,358,021 + -0.9 4,358,446 + Pf1N1B4_3919 0.65 +0.7 4,358,579 + Pf1N1B4_3919 0.75 -0.3 4,358,579 + Pf1N1B4_3919 0.75 -0.3 4,358,580 - -1.3 4,358,843 + -1.6 4,359,016 + +1.1 4,359,169 - Pf1N1B4_3920 0.27 +0.5 4,359,169 - Pf1N1B4_3920 0.27 +0.1 4,359,204 + Pf1N1B4_3920 0.32 +1.1 4,359,474 + Pf1N1B4_3920 0.69 +0.7 4,359,496 + Pf1N1B4_3920 0.72 -0.5 4,359,507 - Pf1N1B4_3920 0.74 -0.9 4,359,507 - Pf1N1B4_3920 0.74 +1.0 4,359,605 + Pf1N1B4_3920 0.88 +1.9 4,359,681 + +0.3 4,359,682 - +0.1 4,359,686 - -0.6 4,359,705 + -1.4 4,359,705 + -0.0 4,359,706 - -0.7 4,359,710 + -1.1 4,359,774 - -0.3 4,359,833 + Pf1N1B4_3921 0.21 -0.3 4,360,279 + +0.3 4,360,279 + +0.3 4,360,279 + -0.9 4,360,279 + -2.6
Or see this region's nucleotide sequence