Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3476 and Pf1N1B4_3477 are separated by 126 nucleotides Pf1N1B4_3477 and Pf1N1B4_3478 are separated by 491 nucleotides Pf1N1B4_3478 and Pf1N1B4_3479 overlap by 4 nucleotides
Pf1N1B4_3476 - Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1), at 3,881,300 to 3,884,194
_3476
Pf1N1B4_3477 - hypothetical protein, at 3,884,321 to 3,884,437
_3477
Pf1N1B4_3478 - Transcriptional regulatory protein RstA, at 3,884,929 to 3,885,651
_3478
Pf1N1B4_3479 - Sensor histidine kinase, at 3,885,648 to 3,887,258
_3479
Position (kb)
3884
3885
3886 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3884.268 kb on - strand at 3884.291 kb on - strand at 3884.308 kb on - strand at 3884.320 kb on - strand at 3884.498 kb on - strand at 3884.563 kb on - strand at 3884.563 kb on - strand at 3884.563 kb on - strand at 3884.569 kb on - strand at 3884.569 kb on - strand at 3884.591 kb on - strand at 3884.598 kb on - strand at 3884.625 kb on - strand at 3884.628 kb on - strand at 3884.680 kb on + strand at 3884.692 kb on + strand at 3884.693 kb on - strand at 3884.799 kb on + strand at 3884.830 kb on - strand at 3884.831 kb on + strand at 3884.832 kb on - strand at 3884.832 kb on - strand at 3884.832 kb on - strand at 3884.859 kb on - strand at 3884.875 kb on - strand at 3884.900 kb on + strand at 3884.998 kb on + strand at 3884.998 kb on + strand at 3884.999 kb on - strand at 3885.086 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.151 kb on + strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.152 kb on - strand, within Pf1N1B4_3478 at 3885.232 kb on + strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.233 kb on - strand, within Pf1N1B4_3478 at 3885.457 kb on - strand, within Pf1N1B4_3478 at 3885.476 kb on + strand, within Pf1N1B4_3478 at 3885.476 kb on + strand, within Pf1N1B4_3478 at 3885.477 kb on - strand, within Pf1N1B4_3478 at 3885.477 kb on - strand, within Pf1N1B4_3478 at 3885.672 kb on + strand at 3885.672 kb on + strand at 3885.672 kb on + strand at 3885.673 kb on - strand at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.068 kb on + strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.069 kb on - strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479 at 3886.320 kb on + strand, within Pf1N1B4_3479
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 3,884,268 - -0.1 3,884,291 - -0.6 3,884,308 - -0.1 3,884,320 - +0.3 3,884,498 - -0.1 3,884,563 - -1.1 3,884,563 - -0.1 3,884,563 - +0.9 3,884,569 - -0.7 3,884,569 - -1.7 3,884,591 - +0.3 3,884,598 - -0.1 3,884,625 - +0.6 3,884,628 - -0.7 3,884,680 + -0.1 3,884,692 + +1.4 3,884,693 - -1.4 3,884,799 + +0.6 3,884,830 - +0.6 3,884,831 + +0.4 3,884,832 - -1.1 3,884,832 - -0.1 3,884,832 - -1.7 3,884,859 - -1.7 3,884,875 - -1.6 3,884,900 + -0.6 3,884,998 + +0.9 3,884,998 + -0.7 3,884,999 - -1.1 3,885,086 + Pf1N1B4_3478 0.22 -1.1 3,885,151 + Pf1N1B4_3478 0.31 +0.1 3,885,151 + Pf1N1B4_3478 0.31 -1.1 3,885,151 + Pf1N1B4_3478 0.31 -0.9 3,885,151 + Pf1N1B4_3478 0.31 -1.1 3,885,151 + Pf1N1B4_3478 0.31 -1.7 3,885,152 - Pf1N1B4_3478 0.31 -2.1 3,885,152 - Pf1N1B4_3478 0.31 -0.1 3,885,152 - Pf1N1B4_3478 0.31 -2.1 3,885,152 - Pf1N1B4_3478 0.31 -2.7 3,885,152 - Pf1N1B4_3478 0.31 -0.1 3,885,152 - Pf1N1B4_3478 0.31 -1.5 3,885,152 - Pf1N1B4_3478 0.31 -0.6 3,885,152 - Pf1N1B4_3478 0.31 -1.1 3,885,152 - Pf1N1B4_3478 0.31 -1.1 3,885,232 + Pf1N1B4_3478 0.42 -2.1 3,885,233 - Pf1N1B4_3478 0.42 +0.9 3,885,233 - Pf1N1B4_3478 0.42 +0.4 3,885,233 - Pf1N1B4_3478 0.42 -0.7 3,885,233 - Pf1N1B4_3478 0.42 -2.1 3,885,457 - Pf1N1B4_3478 0.73 -2.7 3,885,476 + Pf1N1B4_3478 0.76 -0.9 3,885,476 + Pf1N1B4_3478 0.76 -0.7 3,885,477 - Pf1N1B4_3478 0.76 -2.9 3,885,477 - Pf1N1B4_3478 0.76 -1.5 3,885,672 + +0.9 3,885,672 + -0.7 3,885,672 + -0.6 3,885,673 - -0.1 3,886,068 + Pf1N1B4_3479 0.26 -1.7 3,886,068 + Pf1N1B4_3479 0.26 +1.1 3,886,068 + Pf1N1B4_3479 0.26 +0.9 3,886,069 - Pf1N1B4_3479 0.26 -1.7 3,886,069 - Pf1N1B4_3479 0.26 -1.1 3,886,069 - Pf1N1B4_3479 0.26 -0.1 3,886,320 + Pf1N1B4_3479 0.42 -1.1 3,886,320 + Pf1N1B4_3479 0.42 -0.1 3,886,320 + Pf1N1B4_3479 0.42 -1.1
Or see this region's nucleotide sequence