Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2843 and Pf1N1B4_2844 are separated by 317 nucleotides Pf1N1B4_2844 and Pf1N1B4_2845 are separated by 204 nucleotides Pf1N1B4_2845 and Pf1N1B4_2846 overlap by 4 nucleotides
Pf1N1B4_2843 - Type IV pilin PilA, at 3,188,418 to 3,188,840
_2843
Pf1N1B4_2844 - Osmotically inducible protein OsmY, at 3,189,158 to 3,189,514
_2844
Pf1N1B4_2845 - FIG00953066: hypothetical protein, at 3,189,719 to 3,190,018
_2845
Pf1N1B4_2846 - putative exported protein, at 3,190,015 to 3,192,960
_2846
Position (kb)
3189
3190
3191 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3188.726 kb on - strand, within Pf1N1B4_2843 at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.799 kb on + strand at 3188.800 kb on - strand at 3188.800 kb on - strand at 3188.800 kb on - strand at 3188.807 kb on + strand at 3188.808 kb on - strand at 3188.834 kb on + strand at 3188.834 kb on + strand at 3188.983 kb on - strand at 3189.142 kb on + strand at 3189.143 kb on - strand at 3189.143 kb on - strand at 3189.151 kb on + strand at 3189.151 kb on + strand at 3189.152 kb on - strand at 3189.157 kb on + strand at 3189.158 kb on - strand at 3189.513 kb on - strand at 3189.552 kb on - strand at 3189.567 kb on + strand at 3189.567 kb on + strand at 3189.567 kb on + strand at 3189.568 kb on - strand at 3189.568 kb on - strand at 3189.568 kb on - strand at 3189.579 kb on + strand at 3189.580 kb on - strand at 3189.652 kb on + strand at 3189.652 kb on + strand at 3189.652 kb on + strand at 3189.653 kb on - strand at 3189.653 kb on - strand at 3189.663 kb on - strand at 3189.709 kb on + strand at 3189.709 kb on + strand at 3189.709 kb on - strand at 3189.710 kb on - strand at 3189.710 kb on - strand at 3189.724 kb on + strand at 3189.725 kb on - strand at 3189.725 kb on - strand at 3189.725 kb on - strand at 3189.775 kb on - strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand, within Pf1N1B4_2845 at 3189.780 kb on + strand at 3189.781 kb on - strand, within Pf1N1B4_2845 at 3189.781 kb on - strand, within Pf1N1B4_2845 at 3189.802 kb on - strand, within Pf1N1B4_2845 at 3189.802 kb on - strand, within Pf1N1B4_2845 at 3189.802 kb on - strand, within Pf1N1B4_2845 at 3189.802 kb on - strand, within Pf1N1B4_2845 at 3189.853 kb on - strand, within Pf1N1B4_2845 at 3189.949 kb on + strand, within Pf1N1B4_2845 at 3189.949 kb on + strand, within Pf1N1B4_2845 at 3189.950 kb on - strand, within Pf1N1B4_2845 at 3189.950 kb on - strand, within Pf1N1B4_2845 at 3189.950 kb on - strand, within Pf1N1B4_2845 at 3190.091 kb on + strand at 3190.091 kb on + strand at 3190.092 kb on - strand at 3190.092 kb on - strand at 3190.130 kb on + strand at 3190.130 kb on + strand at 3190.131 kb on - strand at 3190.131 kb on - strand at 3190.131 kb on - strand at 3190.131 kb on - strand at 3190.131 kb on - strand at 3190.289 kb on + strand at 3190.289 kb on + strand at 3190.289 kb on + strand at 3190.290 kb on - strand at 3190.355 kb on - strand, within Pf1N1B4_2846 at 3190.385 kb on + strand, within Pf1N1B4_2846 at 3190.385 kb on + strand, within Pf1N1B4_2846 at 3190.385 kb on + strand, within Pf1N1B4_2846 at 3190.386 kb on - strand, within Pf1N1B4_2846 at 3190.386 kb on - strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.388 kb on + strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.389 kb on - strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.394 kb on + strand, within Pf1N1B4_2846 at 3190.395 kb on - strand, within Pf1N1B4_2846 at 3190.400 kb on + strand, within Pf1N1B4_2846 at 3190.401 kb on - strand, within Pf1N1B4_2846 at 3190.401 kb on - strand, within Pf1N1B4_2846 at 3190.671 kb on + strand, within Pf1N1B4_2846 at 3190.671 kb on + strand, within Pf1N1B4_2846 at 3190.671 kb on + strand, within Pf1N1B4_2846 at 3190.671 kb on + strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.672 kb on - strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.674 kb on + strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.675 kb on - strand, within Pf1N1B4_2846 at 3190.877 kb on + strand, within Pf1N1B4_2846 at 3190.877 kb on + strand, within Pf1N1B4_2846 at 3190.877 kb on + strand, within Pf1N1B4_2846 at 3190.878 kb on - strand, within Pf1N1B4_2846 at 3190.878 kb on - strand, within Pf1N1B4_2846 at 3190.878 kb on - strand, within Pf1N1B4_2846 at 3190.937 kb on + strand, within Pf1N1B4_2846 at 3190.937 kb on + strand, within Pf1N1B4_2846 at 3190.938 kb on - strand, within Pf1N1B4_2846
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 3,188,726 - Pf1N1B4_2843 0.73 -1.1 3,188,799 + -1.8 3,188,799 + -1.6 3,188,799 + -0.6 3,188,799 + +0.6 3,188,799 + -0.4 3,188,799 + +0.2 3,188,799 + +0.8 3,188,800 - +1.2 3,188,800 - +0.2 3,188,800 - +2.5 3,188,807 + +0.2 3,188,808 - +0.5 3,188,834 + -0.8 3,188,834 + -0.9 3,188,983 - +2.2 3,189,142 + -0.7 3,189,143 - +0.6 3,189,143 - +0.2 3,189,151 + -0.9 3,189,151 + +0.2 3,189,152 - +0.8 3,189,157 + -0.4 3,189,158 - +0.8 3,189,513 - -0.8 3,189,552 - +0.2 3,189,567 + +1.2 3,189,567 + -0.1 3,189,567 + -1.4 3,189,568 - +0.2 3,189,568 - -1.4 3,189,568 - +1.0 3,189,579 + -0.4 3,189,580 - -0.4 3,189,652 + +0.5 3,189,652 + -2.4 3,189,652 + +0.2 3,189,653 - -2.1 3,189,653 - +0.2 3,189,663 - -0.1 3,189,709 + +0.6 3,189,709 + +1.2 3,189,709 - +1.2 3,189,710 - -0.3 3,189,710 - +0.2 3,189,724 + -2.1 3,189,725 - +0.4 3,189,725 - -0.2 3,189,725 - -0.4 3,189,775 - Pf1N1B4_2845 0.19 -1.4 3,189,780 + Pf1N1B4_2845 0.20 +0.2 3,189,780 + Pf1N1B4_2845 0.20 +0.2 3,189,780 + Pf1N1B4_2845 0.20 -2.6 3,189,780 + Pf1N1B4_2845 0.20 +2.2 3,189,780 + Pf1N1B4_2845 0.20 -1.6 3,189,780 + +1.8 3,189,781 - Pf1N1B4_2845 0.21 -0.8 3,189,781 - Pf1N1B4_2845 0.21 -0.8 3,189,802 - Pf1N1B4_2845 0.28 -2.1 3,189,802 - Pf1N1B4_2845 0.28 -0.8 3,189,802 - Pf1N1B4_2845 0.28 +0.8 3,189,802 - Pf1N1B4_2845 0.28 +0.2 3,189,853 - Pf1N1B4_2845 0.45 +1.2 3,189,949 + Pf1N1B4_2845 0.77 -0.7 3,189,949 + Pf1N1B4_2845 0.77 -2.0 3,189,950 - Pf1N1B4_2845 0.77 +2.8 3,189,950 - Pf1N1B4_2845 0.77 -2.4 3,189,950 - Pf1N1B4_2845 0.77 -1.9 3,190,091 + -0.7 3,190,091 + -0.8 3,190,092 - -0.1 3,190,092 - -1.4 3,190,130 + -0.1 3,190,130 + +1.8 3,190,131 - -0.4 3,190,131 - -2.1 3,190,131 - +0.2 3,190,131 - -0.3 3,190,131 - +1.2 3,190,289 + +0.2 3,190,289 + +0.9 3,190,289 + -2.1 3,190,290 - -1.8 3,190,355 - Pf1N1B4_2846 0.12 -1.8 3,190,385 + Pf1N1B4_2846 0.13 -0.4 3,190,385 + Pf1N1B4_2846 0.13 +1.4 3,190,385 + Pf1N1B4_2846 0.13 -0.8 3,190,386 - Pf1N1B4_2846 0.13 -0.2 3,190,386 - Pf1N1B4_2846 0.13 +0.8 3,190,388 + Pf1N1B4_2846 0.13 -0.2 3,190,388 + Pf1N1B4_2846 0.13 +1.2 3,190,388 + Pf1N1B4_2846 0.13 +1.4 3,190,388 + Pf1N1B4_2846 0.13 +2.2 3,190,388 + Pf1N1B4_2846 0.13 +0.2 3,190,389 - Pf1N1B4_2846 0.13 +0.9 3,190,389 - Pf1N1B4_2846 0.13 -0.1 3,190,389 - Pf1N1B4_2846 0.13 -1.1 3,190,389 - Pf1N1B4_2846 0.13 +0.2 3,190,389 - Pf1N1B4_2846 0.13 +1.8 3,190,389 - Pf1N1B4_2846 0.13 +0.1 3,190,394 + Pf1N1B4_2846 0.13 +0.2 3,190,394 + Pf1N1B4_2846 0.13 +0.8 3,190,394 + Pf1N1B4_2846 0.13 +0.3 3,190,394 + Pf1N1B4_2846 0.13 +1.9 3,190,394 + Pf1N1B4_2846 0.13 -0.4 3,190,394 + Pf1N1B4_2846 0.13 +0.2 3,190,395 - Pf1N1B4_2846 0.13 -0.2 3,190,400 + Pf1N1B4_2846 0.13 -0.2 3,190,401 - Pf1N1B4_2846 0.13 -1.2 3,190,401 - Pf1N1B4_2846 0.13 +0.5 3,190,671 + Pf1N1B4_2846 0.22 -0.8 3,190,671 + Pf1N1B4_2846 0.22 -0.2 3,190,671 + Pf1N1B4_2846 0.22 -1.8 3,190,671 + Pf1N1B4_2846 0.22 -0.2 3,190,672 - Pf1N1B4_2846 0.22 -0.2 3,190,672 - Pf1N1B4_2846 0.22 +1.5 3,190,672 - Pf1N1B4_2846 0.22 +0.2 3,190,672 - Pf1N1B4_2846 0.22 -2.6 3,190,672 - Pf1N1B4_2846 0.22 +0.8 3,190,674 + Pf1N1B4_2846 0.22 -1.4 3,190,674 + Pf1N1B4_2846 0.22 -0.5 3,190,674 + Pf1N1B4_2846 0.22 +0.2 3,190,674 + Pf1N1B4_2846 0.22 +1.8 3,190,674 + Pf1N1B4_2846 0.22 -2.6 3,190,675 - Pf1N1B4_2846 0.22 +2.0 3,190,675 - Pf1N1B4_2846 0.22 -2.1 3,190,675 - Pf1N1B4_2846 0.22 +2.5 3,190,675 - Pf1N1B4_2846 0.22 +2.2 3,190,675 - Pf1N1B4_2846 0.22 +1.2 3,190,877 + Pf1N1B4_2846 0.29 +1.1 3,190,877 + Pf1N1B4_2846 0.29 +0.2 3,190,877 + Pf1N1B4_2846 0.29 -0.1 3,190,878 - Pf1N1B4_2846 0.29 +0.2 3,190,878 - Pf1N1B4_2846 0.29 +0.9 3,190,878 - Pf1N1B4_2846 0.29 +0.6 3,190,937 + Pf1N1B4_2846 0.31 -0.1 3,190,937 + Pf1N1B4_2846 0.31 -0.5 3,190,938 - Pf1N1B4_2846 0.31 -1.0
Or see this region's nucleotide sequence