Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1948 and Pf1N1B4_1949 are separated by 1 nucleotides Pf1N1B4_1949 and Pf1N1B4_1950 are separated by 11 nucleotides Pf1N1B4_1950 and Pf1N1B4_1951 overlap by 11 nucleotides
Pf1N1B4_1948 - Phosphatidate cytidylyltransferase (EC 2.7.7.41), at 2,229,314 to 2,230,246
_1948
Pf1N1B4_1949 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 2,230,248 to 2,230,868
_1949
Pf1N1B4_1950 - FIG00955422: hypothetical protein, at 2,230,880 to 2,231,335
_1950
Pf1N1B4_1951 - Ser/Thr and Tyr protein phosphatase (dual specificity), at 2,231,325 to 2,232,605
_1951
Position (kb)
2230
2231 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2229.311 kb on - strand at 2229.654 kb on + strand, within Pf1N1B4_1948 at 2229.654 kb on + strand, within Pf1N1B4_1948 at 2229.655 kb on - strand, within Pf1N1B4_1948 at 2229.655 kb on - strand, within Pf1N1B4_1948 at 2229.655 kb on - strand, within Pf1N1B4_1948 at 2229.655 kb on - strand, within Pf1N1B4_1948 at 2229.655 kb on - strand, within Pf1N1B4_1948 at 2229.655 kb on - strand, within Pf1N1B4_1948 at 2229.693 kb on + strand, within Pf1N1B4_1948 at 2229.869 kb on + strand, within Pf1N1B4_1948 at 2229.869 kb on + strand, within Pf1N1B4_1948 at 2229.869 kb on + strand, within Pf1N1B4_1948 at 2229.869 kb on + strand, within Pf1N1B4_1948 at 2229.869 kb on + strand, within Pf1N1B4_1948 at 2229.870 kb on - strand, within Pf1N1B4_1948 at 2229.870 kb on - strand, within Pf1N1B4_1948 at 2229.870 kb on - strand, within Pf1N1B4_1948 at 2229.873 kb on + strand, within Pf1N1B4_1948 at 2229.873 kb on + strand, within Pf1N1B4_1948 at 2229.874 kb on - strand at 2229.874 kb on - strand, within Pf1N1B4_1948 at 2229.874 kb on - strand, within Pf1N1B4_1948 at 2229.883 kb on + strand, within Pf1N1B4_1948 at 2229.883 kb on + strand, within Pf1N1B4_1948 at 2229.884 kb on - strand, within Pf1N1B4_1948 at 2229.884 kb on - strand, within Pf1N1B4_1948 at 2229.915 kb on + strand, within Pf1N1B4_1948 at 2229.915 kb on + strand, within Pf1N1B4_1948 at 2229.915 kb on + strand, within Pf1N1B4_1948 at 2229.915 kb on + strand, within Pf1N1B4_1948 at 2229.916 kb on - strand, within Pf1N1B4_1948 at 2229.927 kb on + strand, within Pf1N1B4_1948 at 2229.927 kb on + strand, within Pf1N1B4_1948 at 2229.927 kb on + strand, within Pf1N1B4_1948 at 2229.927 kb on + strand, within Pf1N1B4_1948 at 2229.928 kb on - strand, within Pf1N1B4_1948 at 2229.928 kb on - strand, within Pf1N1B4_1948 at 2229.928 kb on - strand, within Pf1N1B4_1948 at 2229.928 kb on - strand, within Pf1N1B4_1948 at 2229.928 kb on - strand, within Pf1N1B4_1948 at 2229.928 kb on - strand, within Pf1N1B4_1948 at 2229.949 kb on - strand, within Pf1N1B4_1948 at 2229.949 kb on - strand, within Pf1N1B4_1948 at 2230.020 kb on + strand, within Pf1N1B4_1948 at 2230.020 kb on + strand, within Pf1N1B4_1948 at 2230.020 kb on + strand, within Pf1N1B4_1948 at 2230.021 kb on - strand, within Pf1N1B4_1948 at 2230.021 kb on - strand, within Pf1N1B4_1948 at 2230.035 kb on + strand, within Pf1N1B4_1948 at 2230.036 kb on - strand, within Pf1N1B4_1948 at 2230.240 kb on + strand at 2230.355 kb on + strand, within Pf1N1B4_1949 at 2230.355 kb on + strand, within Pf1N1B4_1949 at 2230.356 kb on - strand, within Pf1N1B4_1949 at 2230.356 kb on - strand, within Pf1N1B4_1949 at 2230.462 kb on + strand, within Pf1N1B4_1949 at 2230.463 kb on - strand, within Pf1N1B4_1949 at 2230.637 kb on - strand, within Pf1N1B4_1949 at 2230.637 kb on - strand, within Pf1N1B4_1949 at 2230.637 kb on - strand, within Pf1N1B4_1949 at 2230.637 kb on - strand, within Pf1N1B4_1949 at 2230.645 kb on - strand, within Pf1N1B4_1949 at 2230.663 kb on + strand, within Pf1N1B4_1949 at 2230.688 kb on + strand, within Pf1N1B4_1949 at 2230.762 kb on + strand, within Pf1N1B4_1949 at 2230.762 kb on + strand, within Pf1N1B4_1949 at 2230.762 kb on + strand, within Pf1N1B4_1949 at 2230.763 kb on - strand, within Pf1N1B4_1949 at 2230.763 kb on - strand, within Pf1N1B4_1949 at 2230.763 kb on - strand, within Pf1N1B4_1949 at 2230.763 kb on - strand, within Pf1N1B4_1949 at 2231.107 kb on + strand, within Pf1N1B4_1950 at 2231.145 kb on + strand, within Pf1N1B4_1950 at 2231.336 kb on - strand at 2231.336 kb on - strand at 2231.600 kb on - strand, within Pf1N1B4_1951 at 2231.637 kb on + strand, within Pf1N1B4_1951 at 2231.637 kb on + strand, within Pf1N1B4_1951 at 2231.637 kb on + strand, within Pf1N1B4_1951 at 2231.637 kb on + strand, within Pf1N1B4_1951 at 2231.637 kb on + strand, within Pf1N1B4_1951 at 2231.638 kb on - strand, within Pf1N1B4_1951 at 2231.638 kb on - strand, within Pf1N1B4_1951 at 2231.638 kb on - strand, within Pf1N1B4_1951 at 2231.638 kb on - strand, within Pf1N1B4_1951 at 2231.773 kb on + strand, within Pf1N1B4_1951 at 2231.773 kb on + strand, within Pf1N1B4_1951 at 2231.773 kb on + strand, within Pf1N1B4_1951 at 2231.773 kb on + strand, within Pf1N1B4_1951 at 2231.774 kb on - strand, within Pf1N1B4_1951 at 2231.774 kb on - strand at 2231.774 kb on - strand, within Pf1N1B4_1951 at 2231.774 kb on - strand, within Pf1N1B4_1951 at 2231.784 kb on + strand, within Pf1N1B4_1951 at 2231.784 kb on + strand, within Pf1N1B4_1951 at 2231.785 kb on - strand, within Pf1N1B4_1951 at 2231.785 kb on - strand, within Pf1N1B4_1951 at 2231.785 kb on - strand, within Pf1N1B4_1951 at 2231.854 kb on + strand, within Pf1N1B4_1951 at 2231.854 kb on + strand, within Pf1N1B4_1951 at 2231.854 kb on + strand, within Pf1N1B4_1951 at 2231.854 kb on + strand at 2231.855 kb on - strand, within Pf1N1B4_1951 at 2231.855 kb on - strand, within Pf1N1B4_1951 at 2231.855 kb on - strand, within Pf1N1B4_1951 at 2231.855 kb on - strand at 2231.855 kb on - strand, within Pf1N1B4_1951 at 2231.855 kb on - strand, within Pf1N1B4_1951
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 2,229,311 - +1.5 2,229,654 + Pf1N1B4_1948 0.36 -0.5 2,229,654 + Pf1N1B4_1948 0.36 +0.5 2,229,655 - Pf1N1B4_1948 0.37 +1.5 2,229,655 - Pf1N1B4_1948 0.37 +0.5 2,229,655 - Pf1N1B4_1948 0.37 +1.5 2,229,655 - Pf1N1B4_1948 0.37 +0.3 2,229,655 - Pf1N1B4_1948 0.37 +1.1 2,229,655 - Pf1N1B4_1948 0.37 -1.5 2,229,693 + Pf1N1B4_1948 0.41 -0.4 2,229,869 + Pf1N1B4_1948 0.59 -0.3 2,229,869 + Pf1N1B4_1948 0.59 -0.8 2,229,869 + Pf1N1B4_1948 0.59 +0.7 2,229,869 + Pf1N1B4_1948 0.59 -0.1 2,229,869 + Pf1N1B4_1948 0.59 +0.5 2,229,870 - Pf1N1B4_1948 0.60 +0.5 2,229,870 - Pf1N1B4_1948 0.60 +0.5 2,229,870 - Pf1N1B4_1948 0.60 -2.3 2,229,873 + Pf1N1B4_1948 0.60 +1.7 2,229,873 + Pf1N1B4_1948 0.60 +0.3 2,229,874 - -1.1 2,229,874 - Pf1N1B4_1948 0.60 +0.8 2,229,874 - Pf1N1B4_1948 0.60 +2.1 2,229,883 + Pf1N1B4_1948 0.61 +0.6 2,229,883 + Pf1N1B4_1948 0.61 +0.5 2,229,884 - Pf1N1B4_1948 0.61 +0.9 2,229,884 - Pf1N1B4_1948 0.61 -0.1 2,229,915 + Pf1N1B4_1948 0.64 +0.1 2,229,915 + Pf1N1B4_1948 0.64 -1.7 2,229,915 + Pf1N1B4_1948 0.64 +0.5 2,229,915 + Pf1N1B4_1948 0.64 -0.5 2,229,916 - Pf1N1B4_1948 0.65 +1.1 2,229,927 + Pf1N1B4_1948 0.66 -0.7 2,229,927 + Pf1N1B4_1948 0.66 +0.0 2,229,927 + Pf1N1B4_1948 0.66 -0.5 2,229,927 + Pf1N1B4_1948 0.66 -0.3 2,229,928 - Pf1N1B4_1948 0.66 -0.1 2,229,928 - Pf1N1B4_1948 0.66 +1.1 2,229,928 - Pf1N1B4_1948 0.66 -0.2 2,229,928 - Pf1N1B4_1948 0.66 -0.2 2,229,928 - Pf1N1B4_1948 0.66 +0.2 2,229,928 - Pf1N1B4_1948 0.66 +0.0 2,229,949 - Pf1N1B4_1948 0.68 +2.5 2,229,949 - Pf1N1B4_1948 0.68 -1.0 2,230,020 + Pf1N1B4_1948 0.76 -1.0 2,230,020 + Pf1N1B4_1948 0.76 -0.5 2,230,020 + Pf1N1B4_1948 0.76 -0.2 2,230,021 - Pf1N1B4_1948 0.76 -0.8 2,230,021 - Pf1N1B4_1948 0.76 -0.3 2,230,035 + Pf1N1B4_1948 0.77 +1.5 2,230,036 - Pf1N1B4_1948 0.77 +1.1 2,230,240 + -1.1 2,230,355 + Pf1N1B4_1949 0.17 -0.3 2,230,355 + Pf1N1B4_1949 0.17 +1.5 2,230,356 - Pf1N1B4_1949 0.17 -0.3 2,230,356 - Pf1N1B4_1949 0.17 +0.5 2,230,462 + Pf1N1B4_1949 0.34 -0.1 2,230,463 - Pf1N1B4_1949 0.35 +1.0 2,230,637 - Pf1N1B4_1949 0.63 -1.1 2,230,637 - Pf1N1B4_1949 0.63 -1.8 2,230,637 - Pf1N1B4_1949 0.63 +1.0 2,230,637 - Pf1N1B4_1949 0.63 -0.1 2,230,645 - Pf1N1B4_1949 0.64 -0.6 2,230,663 + Pf1N1B4_1949 0.67 +0.2 2,230,688 + Pf1N1B4_1949 0.71 -1.0 2,230,762 + Pf1N1B4_1949 0.83 -0.3 2,230,762 + Pf1N1B4_1949 0.83 -0.1 2,230,762 + Pf1N1B4_1949 0.83 -0.9 2,230,763 - Pf1N1B4_1949 0.83 +0.1 2,230,763 - Pf1N1B4_1949 0.83 -0.5 2,230,763 - Pf1N1B4_1949 0.83 +0.5 2,230,763 - Pf1N1B4_1949 0.83 -0.2 2,231,107 + Pf1N1B4_1950 0.50 -0.2 2,231,145 + Pf1N1B4_1950 0.58 +1.8 2,231,336 - +0.5 2,231,336 - -0.3 2,231,600 - Pf1N1B4_1951 0.21 -0.8 2,231,637 + Pf1N1B4_1951 0.24 +2.1 2,231,637 + Pf1N1B4_1951 0.24 +2.1 2,231,637 + Pf1N1B4_1951 0.24 -1.5 2,231,637 + Pf1N1B4_1951 0.24 +0.5 2,231,637 + Pf1N1B4_1951 0.24 -1.2 2,231,638 - Pf1N1B4_1951 0.24 -1.1 2,231,638 - Pf1N1B4_1951 0.24 +1.1 2,231,638 - Pf1N1B4_1951 0.24 -0.1 2,231,638 - Pf1N1B4_1951 0.24 +1.3 2,231,773 + Pf1N1B4_1951 0.35 -0.3 2,231,773 + Pf1N1B4_1951 0.35 -0.4 2,231,773 + Pf1N1B4_1951 0.35 -0.5 2,231,773 + Pf1N1B4_1951 0.35 +0.5 2,231,774 - Pf1N1B4_1951 0.35 -1.2 2,231,774 - +1.5 2,231,774 - Pf1N1B4_1951 0.35 -0.0 2,231,774 - Pf1N1B4_1951 0.35 -0.5 2,231,784 + Pf1N1B4_1951 0.36 +0.9 2,231,784 + Pf1N1B4_1951 0.36 -1.5 2,231,785 - Pf1N1B4_1951 0.36 -0.5 2,231,785 - Pf1N1B4_1951 0.36 -0.7 2,231,785 - Pf1N1B4_1951 0.36 -0.1 2,231,854 + Pf1N1B4_1951 0.41 -0.7 2,231,854 + Pf1N1B4_1951 0.41 -0.5 2,231,854 + Pf1N1B4_1951 0.41 -0.5 2,231,854 + +1.5 2,231,855 - Pf1N1B4_1951 0.41 +1.8 2,231,855 - Pf1N1B4_1951 0.41 +0.7 2,231,855 - Pf1N1B4_1951 0.41 +0.5 2,231,855 - +1.5 2,231,855 - Pf1N1B4_1951 0.41 +0.5 2,231,855 - Pf1N1B4_1951 0.41 -0.5
Or see this region's nucleotide sequence