Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1205 and Pf1N1B4_1206 overlap by 36 nucleotides Pf1N1B4_1206 and Pf1N1B4_1207 are separated by 13 nucleotides Pf1N1B4_1207 and Pf1N1B4_1208 are separated by 72 nucleotides Pf1N1B4_1208 and Pf1N1B4_1209 are separated by 132 nucleotides
Pf1N1B4_1205 - Sulfate permease, at 1,369,035 to 1,370,420
_1205
Pf1N1B4_1206 - hypothetical protein, at 1,370,385 to 1,370,672
_1206
Pf1N1B4_1207 - Transcriptional regulator, LysR family, at 1,370,686 to 1,371,597
_1207
Pf1N1B4_1208 - Modulator of drug activity B, at 1,371,670 to 1,372,260
_1208
Pf1N1B4_1209 - FIG00959330: hypothetical protein, at 1,372,393 to 1,372,737
_1209
Position (kb)
1370
1371
1372 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1369.814 kb on - strand, within Pf1N1B4_1205 at 1369.814 kb on - strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.047 kb on + strand, within Pf1N1B4_1205 at 1370.048 kb on - strand, within Pf1N1B4_1205 at 1370.048 kb on - strand at 1370.239 kb on + strand, within Pf1N1B4_1205 at 1370.239 kb on + strand, within Pf1N1B4_1205 at 1370.239 kb on + strand, within Pf1N1B4_1205 at 1370.239 kb on + strand, within Pf1N1B4_1205 at 1370.240 kb on - strand, within Pf1N1B4_1205 at 1370.240 kb on - strand, within Pf1N1B4_1205 at 1370.240 kb on - strand, within Pf1N1B4_1205 at 1370.240 kb on - strand, within Pf1N1B4_1205 at 1370.362 kb on + strand at 1370.362 kb on + strand at 1370.363 kb on - strand at 1370.486 kb on - strand, within Pf1N1B4_1206 at 1370.490 kb on - strand, within Pf1N1B4_1206 at 1370.712 kb on - strand at 1370.784 kb on - strand, within Pf1N1B4_1207 at 1370.784 kb on - strand, within Pf1N1B4_1207 at 1370.784 kb on - strand, within Pf1N1B4_1207 at 1370.981 kb on + strand, within Pf1N1B4_1207 at 1370.981 kb on + strand, within Pf1N1B4_1207 at 1370.982 kb on - strand at 1371.083 kb on + strand, within Pf1N1B4_1207 at 1371.084 kb on - strand, within Pf1N1B4_1207 at 1371.266 kb on + strand, within Pf1N1B4_1207 at 1371.266 kb on + strand, within Pf1N1B4_1207 at 1371.267 kb on - strand, within Pf1N1B4_1207 at 1371.404 kb on + strand, within Pf1N1B4_1207 at 1371.404 kb on + strand, within Pf1N1B4_1207 at 1371.404 kb on + strand, within Pf1N1B4_1207 at 1371.405 kb on - strand, within Pf1N1B4_1207 at 1371.405 kb on - strand, within Pf1N1B4_1207 at 1371.405 kb on - strand, within Pf1N1B4_1207 at 1371.655 kb on + strand at 1371.655 kb on + strand at 1371.655 kb on + strand at 1371.655 kb on + strand at 1371.659 kb on - strand at 1371.711 kb on - strand at 1371.711 kb on - strand at 1371.748 kb on + strand, within Pf1N1B4_1208 at 1371.748 kb on + strand, within Pf1N1B4_1208 at 1371.749 kb on - strand, within Pf1N1B4_1208 at 1371.749 kb on - strand, within Pf1N1B4_1208 at 1371.795 kb on + strand, within Pf1N1B4_1208 at 1371.796 kb on - strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.819 kb on - strand, within Pf1N1B4_1208 at 1371.819 kb on - strand, within Pf1N1B4_1208 at 1371.903 kb on + strand, within Pf1N1B4_1208 at 1371.903 kb on + strand, within Pf1N1B4_1208 at 1371.903 kb on + strand, within Pf1N1B4_1208 at 1371.903 kb on + strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.905 kb on + strand, within Pf1N1B4_1208 at 1371.905 kb on + strand, within Pf1N1B4_1208 at 1371.905 kb on + strand, within Pf1N1B4_1208 at 1371.905 kb on + strand, within Pf1N1B4_1208 at 1371.906 kb on - strand, within Pf1N1B4_1208 at 1371.906 kb on - strand, within Pf1N1B4_1208 at 1371.906 kb on - strand, within Pf1N1B4_1208 at 1371.938 kb on + strand, within Pf1N1B4_1208 at 1371.939 kb on - strand, within Pf1N1B4_1208 at 1372.019 kb on + strand, within Pf1N1B4_1208 at 1372.019 kb on + strand, within Pf1N1B4_1208 at 1372.020 kb on - strand, within Pf1N1B4_1208 at 1372.067 kb on + strand, within Pf1N1B4_1208 at 1372.067 kb on + strand, within Pf1N1B4_1208 at 1372.068 kb on - strand, within Pf1N1B4_1208 at 1372.068 kb on - strand, within Pf1N1B4_1208 at 1372.068 kb on - strand, within Pf1N1B4_1208 at 1372.144 kb on + strand, within Pf1N1B4_1208 at 1372.144 kb on + strand, within Pf1N1B4_1208 at 1372.144 kb on + strand, within Pf1N1B4_1208 at 1372.145 kb on - strand, within Pf1N1B4_1208 at 1372.145 kb on - strand, within Pf1N1B4_1208 at 1372.145 kb on - strand, within Pf1N1B4_1208 at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.324 kb on - strand at 1372.324 kb on - strand at 1372.324 kb on - strand at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.505 kb on - strand, within Pf1N1B4_1209 at 1372.505 kb on - strand, within Pf1N1B4_1209 at 1372.505 kb on - strand, within Pf1N1B4_1209 at 1372.505 kb on - strand, within Pf1N1B4_1209 at 1372.581 kb on + strand, within Pf1N1B4_1209 at 1372.581 kb on + strand, within Pf1N1B4_1209 at 1372.582 kb on - strand, within Pf1N1B4_1209 at 1372.588 kb on + strand at 1372.588 kb on + strand, within Pf1N1B4_1209 at 1372.589 kb on - strand, within Pf1N1B4_1209
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 1,369,814 - Pf1N1B4_1205 0.56 +2.2 1,369,814 - Pf1N1B4_1205 0.56 +2.2 1,370,047 + Pf1N1B4_1205 0.73 -0.8 1,370,047 + Pf1N1B4_1205 0.73 +0.9 1,370,047 + Pf1N1B4_1205 0.73 +0.6 1,370,047 + Pf1N1B4_1205 0.73 -1.4 1,370,047 + Pf1N1B4_1205 0.73 +1.8 1,370,047 + Pf1N1B4_1205 0.73 +0.1 1,370,048 - Pf1N1B4_1205 0.73 +1.8 1,370,048 - +0.2 1,370,239 + Pf1N1B4_1205 0.87 +1.9 1,370,239 + Pf1N1B4_1205 0.87 +0.2 1,370,239 + Pf1N1B4_1205 0.87 +2.0 1,370,239 + Pf1N1B4_1205 0.87 -0.8 1,370,240 - Pf1N1B4_1205 0.87 -0.8 1,370,240 - Pf1N1B4_1205 0.87 -1.5 1,370,240 - Pf1N1B4_1205 0.87 +0.8 1,370,240 - Pf1N1B4_1205 0.87 +0.0 1,370,362 + +1.2 1,370,362 + -0.4 1,370,363 - +0.2 1,370,486 - Pf1N1B4_1206 0.35 -1.0 1,370,490 - Pf1N1B4_1206 0.36 -1.8 1,370,712 - -1.1 1,370,784 - Pf1N1B4_1207 0.11 +1.8 1,370,784 - Pf1N1B4_1207 0.11 -1.6 1,370,784 - Pf1N1B4_1207 0.11 -0.4 1,370,981 + Pf1N1B4_1207 0.32 -2.8 1,370,981 + Pf1N1B4_1207 0.32 +1.5 1,370,982 - +1.8 1,371,083 + Pf1N1B4_1207 0.44 +1.2 1,371,084 - Pf1N1B4_1207 0.44 +0.2 1,371,266 + Pf1N1B4_1207 0.64 -0.2 1,371,266 + Pf1N1B4_1207 0.64 +1.2 1,371,267 - Pf1N1B4_1207 0.64 +1.3 1,371,404 + Pf1N1B4_1207 0.79 +0.4 1,371,404 + Pf1N1B4_1207 0.79 -0.8 1,371,404 + Pf1N1B4_1207 0.79 +0.2 1,371,405 - Pf1N1B4_1207 0.79 +2.5 1,371,405 - Pf1N1B4_1207 0.79 -0.4 1,371,405 - Pf1N1B4_1207 0.79 +1.2 1,371,655 + +0.2 1,371,655 + -1.1 1,371,655 + -0.3 1,371,655 + -0.1 1,371,659 - +0.8 1,371,711 - -0.2 1,371,711 - +0.1 1,371,748 + Pf1N1B4_1208 0.13 +0.9 1,371,748 + Pf1N1B4_1208 0.13 +1.8 1,371,749 - Pf1N1B4_1208 0.13 +1.4 1,371,749 - Pf1N1B4_1208 0.13 -2.6 1,371,795 + Pf1N1B4_1208 0.21 -1.1 1,371,796 - Pf1N1B4_1208 0.21 -0.4 1,371,818 + Pf1N1B4_1208 0.25 +1.0 1,371,818 + Pf1N1B4_1208 0.25 -0.8 1,371,818 + Pf1N1B4_1208 0.25 +2.8 1,371,818 + Pf1N1B4_1208 0.25 -0.5 1,371,818 + Pf1N1B4_1208 0.25 -0.0 1,371,818 + Pf1N1B4_1208 0.25 +1.2 1,371,818 + Pf1N1B4_1208 0.25 -0.6 1,371,819 - Pf1N1B4_1208 0.25 +1.8 1,371,819 - Pf1N1B4_1208 0.25 -1.4 1,371,903 + Pf1N1B4_1208 0.39 -0.6 1,371,903 + Pf1N1B4_1208 0.39 +1.0 1,371,903 + Pf1N1B4_1208 0.39 +1.8 1,371,903 + Pf1N1B4_1208 0.39 -0.8 1,371,904 - Pf1N1B4_1208 0.40 +0.1 1,371,904 - Pf1N1B4_1208 0.40 -0.8 1,371,904 - Pf1N1B4_1208 0.40 -1.4 1,371,904 - Pf1N1B4_1208 0.40 +0.2 1,371,904 - Pf1N1B4_1208 0.40 +0.8 1,371,904 - Pf1N1B4_1208 0.40 +0.6 1,371,904 - Pf1N1B4_1208 0.40 +1.0 1,371,904 - Pf1N1B4_1208 0.40 +0.3 1,371,904 - Pf1N1B4_1208 0.40 -0.1 1,371,905 + Pf1N1B4_1208 0.40 +1.4 1,371,905 + Pf1N1B4_1208 0.40 -0.8 1,371,905 + Pf1N1B4_1208 0.40 -2.1 1,371,905 + Pf1N1B4_1208 0.40 +1.5 1,371,906 - Pf1N1B4_1208 0.40 +0.2 1,371,906 - Pf1N1B4_1208 0.40 -0.8 1,371,906 - Pf1N1B4_1208 0.40 -1.8 1,371,938 + Pf1N1B4_1208 0.45 +0.2 1,371,939 - Pf1N1B4_1208 0.46 +0.6 1,372,019 + Pf1N1B4_1208 0.59 -0.6 1,372,019 + Pf1N1B4_1208 0.59 +0.2 1,372,020 - Pf1N1B4_1208 0.59 -1.8 1,372,067 + Pf1N1B4_1208 0.67 -0.2 1,372,067 + Pf1N1B4_1208 0.67 -1.4 1,372,068 - Pf1N1B4_1208 0.67 -0.0 1,372,068 - Pf1N1B4_1208 0.67 +0.3 1,372,068 - Pf1N1B4_1208 0.67 +0.6 1,372,144 + Pf1N1B4_1208 0.80 -0.7 1,372,144 + Pf1N1B4_1208 0.80 -1.4 1,372,144 + Pf1N1B4_1208 0.80 -1.4 1,372,145 - Pf1N1B4_1208 0.80 -1.8 1,372,145 - Pf1N1B4_1208 0.80 +1.2 1,372,145 - Pf1N1B4_1208 0.80 -0.4 1,372,323 + +0.2 1,372,323 + -0.4 1,372,323 + +0.6 1,372,323 + -0.1 1,372,323 + -2.8 1,372,323 + +1.0 1,372,323 + +0.2 1,372,324 - +0.4 1,372,324 - -1.4 1,372,324 - -2.0 1,372,504 + Pf1N1B4_1209 0.32 -1.5 1,372,504 + Pf1N1B4_1209 0.32 +0.5 1,372,504 + Pf1N1B4_1209 0.32 +1.2 1,372,504 + Pf1N1B4_1209 0.32 -0.5 1,372,504 + Pf1N1B4_1209 0.32 -0.8 1,372,504 + Pf1N1B4_1209 0.32 -0.3 1,372,504 + Pf1N1B4_1209 0.32 -1.2 1,372,504 + Pf1N1B4_1209 0.32 -0.1 1,372,504 + Pf1N1B4_1209 0.32 -0.5 1,372,504 + Pf1N1B4_1209 0.32 -1.1 1,372,505 - Pf1N1B4_1209 0.32 +0.8 1,372,505 - Pf1N1B4_1209 0.32 -1.4 1,372,505 - Pf1N1B4_1209 0.32 +0.2 1,372,505 - Pf1N1B4_1209 0.32 +1.2 1,372,581 + Pf1N1B4_1209 0.54 +0.7 1,372,581 + Pf1N1B4_1209 0.54 -0.8 1,372,582 - Pf1N1B4_1209 0.55 -0.2 1,372,588 + +1.5 1,372,588 + Pf1N1B4_1209 0.57 -1.8 1,372,589 - Pf1N1B4_1209 0.57 +1.2
Or see this region's nucleotide sequence