Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_114 and Pf1N1B4_115 are separated by 169 nucleotides Pf1N1B4_115 and Pf1N1B4_116 are separated by 113 nucleotides
Pf1N1B4_114 - Diaminopimelate epimerase (EC 5.1.1.7), at 118,869 to 119,858
_114
Pf1N1B4_115 - Membrane protein mosC, at 120,028 to 121,179
_115
Pf1N1B4_116 - Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein, at 121,293 to 123,725
_116
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 119.164 kb on + strand, within Pf1N1B4_114 at 119.164 kb on + strand, within Pf1N1B4_114 at 119.165 kb on - strand, within Pf1N1B4_114 at 119.165 kb on - strand, within Pf1N1B4_114 at 119.165 kb on - strand, within Pf1N1B4_114 at 119.693 kb on + strand, within Pf1N1B4_114 at 119.694 kb on - strand, within Pf1N1B4_114 at 119.824 kb on + strand at 119.825 kb on - strand at 119.825 kb on - strand at 119.825 kb on - strand at 119.828 kb on + strand at 119.829 kb on - strand at 119.829 kb on - strand at 119.829 kb on - strand at 119.829 kb on - strand at 119.845 kb on + strand at 119.846 kb on - strand at 119.846 kb on - strand at 119.872 kb on - strand at 119.872 kb on - strand at 119.872 kb on - strand at 120.027 kb on + strand at 120.145 kb on + strand, within Pf1N1B4_115 at 120.330 kb on + strand, within Pf1N1B4_115 at 120.843 kb on + strand, within Pf1N1B4_115 at 120.979 kb on + strand, within Pf1N1B4_115 at 121.049 kb on - strand, within Pf1N1B4_115 at 121.177 kb on + strand at 121.178 kb on - strand at 121.205 kb on + strand at 121.205 kb on + strand at 121.210 kb on + strand at 121.210 kb on + strand at 121.281 kb on + strand at 121.342 kb on + strand at 121.342 kb on + strand at 121.342 kb on + strand at 121.342 kb on + strand at 121.342 kb on + strand at 121.343 kb on - strand at 121.343 kb on - strand at 121.343 kb on - strand at 121.378 kb on + strand at 121.378 kb on + strand at 121.379 kb on - strand at 121.522 kb on + strand at 121.522 kb on + strand at 121.523 kb on - strand at 121.523 kb on - strand at 121.604 kb on - strand, within Pf1N1B4_116 at 121.604 kb on - strand, within Pf1N1B4_116 at 121.604 kb on - strand, within Pf1N1B4_116 at 121.658 kb on - strand, within Pf1N1B4_116 at 121.658 kb on - strand, within Pf1N1B4_116 at 121.666 kb on - strand, within Pf1N1B4_116 at 121.718 kb on - strand, within Pf1N1B4_116 at 121.849 kb on + strand, within Pf1N1B4_116 at 121.850 kb on - strand, within Pf1N1B4_116 at 121.876 kb on + strand, within Pf1N1B4_116 at 121.876 kb on + strand, within Pf1N1B4_116 at 121.876 kb on + strand, within Pf1N1B4_116 at 121.915 kb on + strand, within Pf1N1B4_116 at 121.933 kb on + strand, within Pf1N1B4_116 at 121.934 kb on - strand, within Pf1N1B4_116 at 122.006 kb on - strand, within Pf1N1B4_116 at 122.068 kb on + strand, within Pf1N1B4_116 at 122.068 kb on + strand, within Pf1N1B4_116 at 122.068 kb on + strand, within Pf1N1B4_116 at 122.068 kb on + strand, within Pf1N1B4_116 at 122.068 kb on + strand, within Pf1N1B4_116 at 122.068 kb on + strand, within Pf1N1B4_116 at 122.069 kb on - strand, within Pf1N1B4_116 at 122.069 kb on - strand, within Pf1N1B4_116 at 122.069 kb on - strand, within Pf1N1B4_116 at 122.069 kb on - strand, within Pf1N1B4_116 at 122.069 kb on - strand, within Pf1N1B4_116
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 119,164 + Pf1N1B4_114 0.30 -0.0 119,164 + Pf1N1B4_114 0.30 -0.8 119,165 - Pf1N1B4_114 0.30 -2.3 119,165 - Pf1N1B4_114 0.30 +0.2 119,165 - Pf1N1B4_114 0.30 +0.7 119,693 + Pf1N1B4_114 0.83 -0.3 119,694 - Pf1N1B4_114 0.83 +2.1 119,824 + +0.6 119,825 - +0.7 119,825 - -0.6 119,825 - +1.0 119,828 + +1.3 119,829 - -0.4 119,829 - -0.3 119,829 - -2.4 119,829 - +0.3 119,845 + -0.3 119,846 - +0.2 119,846 - -0.3 119,872 - -0.3 119,872 - +1.3 119,872 - -1.3 120,027 + +1.3 120,145 + Pf1N1B4_115 0.10 -1.1 120,330 + Pf1N1B4_115 0.26 -0.3 120,843 + Pf1N1B4_115 0.71 -0.8 120,979 + Pf1N1B4_115 0.83 -0.3 121,049 - Pf1N1B4_115 0.89 -0.7 121,177 + -0.3 121,178 - -0.3 121,205 + +0.7 121,205 + -0.1 121,210 + +0.6 121,210 + -0.3 121,281 + +0.3 121,342 + -1.1 121,342 + -2.3 121,342 + +0.8 121,342 + +0.5 121,342 + +1.1 121,343 - -0.3 121,343 - -1.3 121,343 - +0.3 121,378 + +0.7 121,378 + +0.7 121,379 - -1.1 121,522 + -0.1 121,522 + -0.3 121,523 - +0.0 121,523 - -0.3 121,604 - Pf1N1B4_116 0.13 -0.3 121,604 - Pf1N1B4_116 0.13 -0.3 121,604 - Pf1N1B4_116 0.13 -0.3 121,658 - Pf1N1B4_116 0.15 +0.5 121,658 - Pf1N1B4_116 0.15 -0.2 121,666 - Pf1N1B4_116 0.15 -1.3 121,718 - Pf1N1B4_116 0.17 -1.8 121,849 + Pf1N1B4_116 0.23 -0.3 121,850 - Pf1N1B4_116 0.23 -0.5 121,876 + Pf1N1B4_116 0.24 +1.1 121,876 + Pf1N1B4_116 0.24 -2.3 121,876 + Pf1N1B4_116 0.24 -0.8 121,915 + Pf1N1B4_116 0.26 -1.0 121,933 + Pf1N1B4_116 0.26 +0.3 121,934 - Pf1N1B4_116 0.26 -0.6 122,006 - Pf1N1B4_116 0.29 -0.3 122,068 + Pf1N1B4_116 0.32 +0.7 122,068 + Pf1N1B4_116 0.32 +1.3 122,068 + Pf1N1B4_116 0.32 -0.3 122,068 + Pf1N1B4_116 0.32 +0.3 122,068 + Pf1N1B4_116 0.32 +2.1 122,068 + Pf1N1B4_116 0.32 -0.8 122,069 - Pf1N1B4_116 0.32 -0.7 122,069 - Pf1N1B4_116 0.32 +0.3 122,069 - Pf1N1B4_116 0.32 +1.0 122,069 - Pf1N1B4_116 0.32 -0.3 122,069 - Pf1N1B4_116 0.32 -0.4
Or see this region's nucleotide sequence