Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3129 and Ac3H11_3130 are separated by 13 nucleotides Ac3H11_3130 and Ac3H11_3131 overlap by 43 nucleotides Ac3H11_3131 and Ac3H11_3132 are separated by 18 nucleotides Ac3H11_3132 and Ac3H11_3133 are separated by 18 nucleotides Ac3H11_3133 and Ac3H11_3134 overlap by 4 nucleotides
Ac3H11_3129 - SAM-dependent methyltransferase (EC 2.1.1.-), at 31,476 to 32,111
_3129
Ac3H11_3130 - Ferredoxin, at 32,125 to 32,421
_3130
Ac3H11_3131 - hypothetical protein, at 32,379 to 32,789
_3131
Ac3H11_3132 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 32,808 to 33,929
_3132
Ac3H11_3133 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 33,948 to 34,412
_3133
Ac3H11_3134 - Transcription termination protein NusB, at 34,409 to 34,951
_3134
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-1
0
1 at 31.899 kb on + strand, within Ac3H11_3129 at 31.899 kb on + strand, within Ac3H11_3129 at 31.899 kb on + strand, within Ac3H11_3129 at 31.899 kb on + strand, within Ac3H11_3129 at 31.899 kb on + strand, within Ac3H11_3129 at 31.900 kb on - strand, within Ac3H11_3129 at 31.900 kb on - strand, within Ac3H11_3129 at 31.900 kb on - strand, within Ac3H11_3129 at 31.900 kb on - strand, within Ac3H11_3129 at 31.900 kb on - strand, within Ac3H11_3129 at 31.900 kb on - strand, within Ac3H11_3129 at 31.900 kb on - strand, within Ac3H11_3129 at 31.900 kb on - strand, within Ac3H11_3129 at 31.909 kb on - strand, within Ac3H11_3129 at 32.180 kb on + strand, within Ac3H11_3130 at 32.180 kb on + strand, within Ac3H11_3130 at 32.180 kb on + strand, within Ac3H11_3130 at 32.180 kb on + strand, within Ac3H11_3130 at 32.180 kb on + strand, within Ac3H11_3130 at 32.180 kb on + strand, within Ac3H11_3130 at 32.180 kb on + strand, within Ac3H11_3130 at 32.181 kb on - strand, within Ac3H11_3130 at 32.181 kb on - strand, within Ac3H11_3130 at 32.181 kb on - strand, within Ac3H11_3130 at 32.181 kb on - strand, within Ac3H11_3130 at 32.311 kb on + strand, within Ac3H11_3130 at 32.312 kb on - strand, within Ac3H11_3130 at 32.312 kb on - strand, within Ac3H11_3130 at 32.471 kb on + strand, within Ac3H11_3131 at 32.577 kb on + strand, within Ac3H11_3131 at 32.577 kb on + strand, within Ac3H11_3131 at 32.577 kb on + strand, within Ac3H11_3131 at 32.644 kb on + strand, within Ac3H11_3131 at 32.644 kb on + strand, within Ac3H11_3131 at 32.644 kb on + strand, within Ac3H11_3131 at 32.644 kb on + strand, within Ac3H11_3131 at 33.069 kb on + strand, within Ac3H11_3132 at 33.069 kb on + strand, within Ac3H11_3132 at 33.666 kb on + strand, within Ac3H11_3132 at 33.666 kb on + strand, within Ac3H11_3132 at 33.666 kb on + strand, within Ac3H11_3132 at 33.801 kb on + strand, within Ac3H11_3132 at 33.801 kb on + strand, within Ac3H11_3132 at 33.941 kb on + strand at 33.947 kb on + strand at 34.218 kb on + strand, within Ac3H11_3133 at 34.218 kb on + strand, within Ac3H11_3133 at 34.218 kb on + strand, within Ac3H11_3133 at 34.278 kb on + strand, within Ac3H11_3133
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 31,899 + Ac3H11_3129 0.67 +0.6 31,899 + Ac3H11_3129 0.67 +0.7 31,899 + Ac3H11_3129 0.67 -0.5 31,899 + Ac3H11_3129 0.67 -0.3 31,899 + Ac3H11_3129 0.67 +0.0 31,900 - Ac3H11_3129 0.67 -0.4 31,900 - Ac3H11_3129 0.67 -0.6 31,900 - Ac3H11_3129 0.67 -1.0 31,900 - Ac3H11_3129 0.67 +0.4 31,900 - Ac3H11_3129 0.67 +0.1 31,900 - Ac3H11_3129 0.67 -1.2 31,900 - Ac3H11_3129 0.67 -1.3 31,900 - Ac3H11_3129 0.67 -0.2 31,909 - Ac3H11_3129 0.68 -0.3 32,180 + Ac3H11_3130 0.19 -0.8 32,180 + Ac3H11_3130 0.19 +0.2 32,180 + Ac3H11_3130 0.19 -0.5 32,180 + Ac3H11_3130 0.19 +0.1 32,180 + Ac3H11_3130 0.19 -0.4 32,180 + Ac3H11_3130 0.19 -0.7 32,180 + Ac3H11_3130 0.19 -0.2 32,181 - Ac3H11_3130 0.19 -0.3 32,181 - Ac3H11_3130 0.19 -0.3 32,181 - Ac3H11_3130 0.19 -0.2 32,181 - Ac3H11_3130 0.19 +0.0 32,311 + Ac3H11_3130 0.63 -0.5 32,312 - Ac3H11_3130 0.63 -0.1 32,312 - Ac3H11_3130 0.63 +0.1 32,471 + Ac3H11_3131 0.22 -0.1 32,577 + Ac3H11_3131 0.48 +0.3 32,577 + Ac3H11_3131 0.48 -0.2 32,577 + Ac3H11_3131 0.48 -0.1 32,644 + Ac3H11_3131 0.64 -0.5 32,644 + Ac3H11_3131 0.64 -0.0 32,644 + Ac3H11_3131 0.64 +0.3 32,644 + Ac3H11_3131 0.64 +0.9 33,069 + Ac3H11_3132 0.23 +0.6 33,069 + Ac3H11_3132 0.23 +0.2 33,666 + Ac3H11_3132 0.76 +0.3 33,666 + Ac3H11_3132 0.76 +0.1 33,666 + Ac3H11_3132 0.76 -0.3 33,801 + Ac3H11_3132 0.89 +0.2 33,801 + Ac3H11_3132 0.89 -0.9 33,941 + +0.0 33,947 + -0.0 34,218 + Ac3H11_3133 0.58 -0.7 34,218 + Ac3H11_3133 0.58 -0.1 34,218 + Ac3H11_3133 0.58 +0.1 34,278 + Ac3H11_3133 0.71 -0.9
Or see this region's nucleotide sequence