Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36060 and RR42_RS36065 are separated by 160 nucleotides RR42_RS36065 and RR42_RS36070 are separated by 212 nucleotides RR42_RS36070 and RR42_RS36075 are separated by 33 nucleotides
RR42_RS36060: RR42_RS36060 - amidase, at 3,540,562 to 3,541,968
_RS36060
RR42_RS36065: RR42_RS36065 - ABC transporter substrate-binding protein, at 3,542,129 to 3,543,085
_RS36065
RR42_RS36070: RR42_RS36070 - hypothetical protein, at 3,543,298 to 3,543,549
_RS36070
RR42_RS36075: RR42_RS36075 - oxidoreductase, at 3,543,583 to 3,544,326
_RS36075
Position (kb)
3542
3543
3544 Strain fitness (log2 ratio)
-2
-1
0
1 at 3541.522 kb on + strand, within RR42_RS36060 at 3541.522 kb on + strand, within RR42_RS36060 at 3541.523 kb on - strand, within RR42_RS36060 at 3541.523 kb on - strand, within RR42_RS36060 at 3541.523 kb on - strand, within RR42_RS36060 at 3541.676 kb on - strand, within RR42_RS36060 at 3541.764 kb on + strand, within RR42_RS36060 at 3541.764 kb on + strand, within RR42_RS36060 at 3541.765 kb on - strand, within RR42_RS36060 at 3541.765 kb on - strand, within RR42_RS36060 at 3541.768 kb on + strand, within RR42_RS36060 at 3541.768 kb on + strand, within RR42_RS36060 at 3541.768 kb on + strand, within RR42_RS36060 at 3541.768 kb on + strand, within RR42_RS36060 at 3541.769 kb on - strand, within RR42_RS36060 at 3541.769 kb on - strand, within RR42_RS36060 at 3541.793 kb on - strand, within RR42_RS36060 at 3541.886 kb on - strand at 3541.886 kb on - strand at 3541.966 kb on + strand at 3541.966 kb on + strand at 3541.967 kb on - strand at 3542.345 kb on + strand, within RR42_RS36065 at 3542.373 kb on - strand, within RR42_RS36065 at 3542.374 kb on + strand, within RR42_RS36065 at 3542.375 kb on - strand, within RR42_RS36065 at 3542.586 kb on - strand, within RR42_RS36065 at 3542.658 kb on + strand, within RR42_RS36065 at 3542.659 kb on - strand, within RR42_RS36065 at 3542.659 kb on - strand, within RR42_RS36065 at 3542.993 kb on + strand at 3543.012 kb on - strand at 3543.218 kb on - strand at 3543.252 kb on + strand at 3543.252 kb on + strand at 3543.252 kb on + strand at 3543.252 kb on + strand at 3543.252 kb on + strand at 3543.252 kb on + strand at 3543.253 kb on - strand at 3543.253 kb on - strand at 3543.253 kb on - strand at 3543.260 kb on - strand at 3543.260 kb on - strand at 3543.260 kb on - strand at 3543.260 kb on - strand at 3543.264 kb on + strand at 3543.264 kb on + strand at 3543.265 kb on - strand at 3543.265 kb on - strand at 3543.624 kb on + strand at 3543.699 kb on - strand, within RR42_RS36075 at 3543.699 kb on - strand, within RR42_RS36075 at 3543.858 kb on - strand, within RR42_RS36075 at 3543.858 kb on - strand, within RR42_RS36075 at 3543.858 kb on - strand, within RR42_RS36075 at 3543.906 kb on + strand, within RR42_RS36075 at 3543.906 kb on + strand, within RR42_RS36075 at 3543.907 kb on - strand, within RR42_RS36075 at 3543.965 kb on + strand, within RR42_RS36075 at 3543.970 kb on + strand, within RR42_RS36075 at 3543.971 kb on - strand, within RR42_RS36075 at 3543.971 kb on - strand, within RR42_RS36075 at 3543.971 kb on - strand, within RR42_RS36075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 3,541,522 + RR42_RS36060 0.68 +0.1 3,541,522 + RR42_RS36060 0.68 +0.2 3,541,523 - RR42_RS36060 0.68 +0.1 3,541,523 - RR42_RS36060 0.68 -0.7 3,541,523 - RR42_RS36060 0.68 -0.4 3,541,676 - RR42_RS36060 0.79 -0.9 3,541,764 + RR42_RS36060 0.85 -0.1 3,541,764 + RR42_RS36060 0.85 -0.5 3,541,765 - RR42_RS36060 0.86 -0.5 3,541,765 - RR42_RS36060 0.86 -0.1 3,541,768 + RR42_RS36060 0.86 +0.2 3,541,768 + RR42_RS36060 0.86 -2.0 3,541,768 + RR42_RS36060 0.86 -1.0 3,541,768 + RR42_RS36060 0.86 +1.3 3,541,769 - RR42_RS36060 0.86 -0.1 3,541,769 - RR42_RS36060 0.86 -2.0 3,541,793 - RR42_RS36060 0.87 +0.3 3,541,886 - -0.4 3,541,886 - +0.3 3,541,966 + +0.3 3,541,966 + -0.7 3,541,967 - -2.2 3,542,345 + RR42_RS36065 0.23 -0.1 3,542,373 - RR42_RS36065 0.25 -0.5 3,542,374 + RR42_RS36065 0.26 +0.4 3,542,375 - RR42_RS36065 0.26 -0.3 3,542,586 - RR42_RS36065 0.48 -1.0 3,542,658 + RR42_RS36065 0.55 +0.5 3,542,659 - RR42_RS36065 0.55 -0.1 3,542,659 - RR42_RS36065 0.55 -0.1 3,542,993 + -0.1 3,543,012 - -0.3 3,543,218 - +0.1 3,543,252 + -0.1 3,543,252 + +0.4 3,543,252 + +0.3 3,543,252 + +0.4 3,543,252 + -0.1 3,543,252 + +0.6 3,543,253 - +0.3 3,543,253 - +0.3 3,543,253 - +0.5 3,543,260 - +1.6 3,543,260 - -0.5 3,543,260 - -0.3 3,543,260 - +0.1 3,543,264 + +0.1 3,543,264 + +0.2 3,543,265 - -0.1 3,543,265 - +0.2 3,543,624 + +0.6 3,543,699 - RR42_RS36075 0.16 +0.8 3,543,699 - RR42_RS36075 0.16 -1.1 3,543,858 - RR42_RS36075 0.37 -0.1 3,543,858 - RR42_RS36075 0.37 +0.6 3,543,858 - RR42_RS36075 0.37 -0.6 3,543,906 + RR42_RS36075 0.43 -0.2 3,543,906 + RR42_RS36075 0.43 -0.8 3,543,907 - RR42_RS36075 0.44 -0.9 3,543,965 + RR42_RS36075 0.51 -0.1 3,543,970 + RR42_RS36075 0.52 +0.2 3,543,971 - RR42_RS36075 0.52 -0.6 3,543,971 - RR42_RS36075 0.52 +0.1 3,543,971 - RR42_RS36075 0.52 -0.3
Or see this region's nucleotide sequence