Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31725 and RR42_RS31730 are separated by 41 nucleotides RR42_RS31730 and RR42_RS31735 overlap by 4 nucleotides RR42_RS31735 and RR42_RS31740 overlap by 4 nucleotides RR42_RS31740 and RR42_RS31745 are separated by 14 nucleotides
RR42_RS31725: RR42_RS31725 - regulator, at 2,494,731 to 2,496,032
_RS31725
RR42_RS31730: RR42_RS31730 - formimidoylglutamase, at 2,496,074 to 2,497,015
_RS31730
RR42_RS31735: RR42_RS31735 - ABC transporter ATP-binding protein, at 2,497,012 to 2,497,773
_RS31735
RR42_RS31740: RR42_RS31740 - ABC transporter permease, at 2,497,770 to 2,498,438
_RS31740
RR42_RS31745: RR42_RS31745 - ABC transporter permease, at 2,498,453 to 2,499,202
_RS31745
Position (kb)
2497
2498 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2496.030 kb on + strand at 2496.030 kb on + strand at 2496.135 kb on + strand at 2496.147 kb on + strand at 2496.148 kb on - strand at 2496.148 kb on - strand at 2496.148 kb on - strand at 2496.212 kb on + strand, within RR42_RS31730 at 2496.297 kb on + strand, within RR42_RS31730 at 2496.297 kb on + strand, within RR42_RS31730 at 2496.297 kb on + strand, within RR42_RS31730 at 2496.298 kb on - strand, within RR42_RS31730 at 2496.298 kb on - strand, within RR42_RS31730 at 2496.344 kb on - strand, within RR42_RS31730 at 2496.384 kb on + strand, within RR42_RS31730 at 2496.384 kb on + strand, within RR42_RS31730 at 2496.384 kb on + strand at 2496.385 kb on - strand, within RR42_RS31730 at 2496.427 kb on + strand, within RR42_RS31730 at 2496.444 kb on - strand, within RR42_RS31730 at 2496.459 kb on + strand, within RR42_RS31730 at 2496.459 kb on + strand, within RR42_RS31730 at 2496.459 kb on + strand, within RR42_RS31730 at 2496.459 kb on + strand, within RR42_RS31730 at 2496.460 kb on - strand, within RR42_RS31730 at 2496.460 kb on - strand, within RR42_RS31730 at 2496.765 kb on + strand, within RR42_RS31730 at 2496.766 kb on - strand, within RR42_RS31730 at 2497.101 kb on + strand, within RR42_RS31735 at 2497.255 kb on - strand, within RR42_RS31735 at 2497.255 kb on - strand, within RR42_RS31735 at 2497.791 kb on - strand at 2497.849 kb on + strand, within RR42_RS31740 at 2497.849 kb on + strand, within RR42_RS31740 at 2497.850 kb on - strand, within RR42_RS31740 at 2497.850 kb on - strand, within RR42_RS31740 at 2497.871 kb on - strand, within RR42_RS31740 at 2497.948 kb on - strand, within RR42_RS31740 at 2497.948 kb on - strand, within RR42_RS31740 at 2498.132 kb on - strand, within RR42_RS31740 at 2498.282 kb on - strand, within RR42_RS31740 at 2498.282 kb on - strand, within RR42_RS31740 at 2498.455 kb on - strand at 2498.455 kb on - strand at 2498.455 kb on - strand at 2498.458 kb on + strand at 2498.458 kb on + strand at 2498.458 kb on + strand at 2498.458 kb on + strand at 2498.497 kb on - strand at 2498.581 kb on - strand, within RR42_RS31745 at 2498.637 kb on + strand, within RR42_RS31745 at 2498.637 kb on + strand, within RR42_RS31745 at 2498.637 kb on + strand, within RR42_RS31745 at 2498.638 kb on - strand, within RR42_RS31745 at 2498.638 kb on - strand, within RR42_RS31745 at 2498.746 kb on - strand, within RR42_RS31745 at 2498.746 kb on - strand, within RR42_RS31745 at 2498.746 kb on - strand, within RR42_RS31745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 2,496,030 + +0.0 2,496,030 + -1.6 2,496,135 + +0.5 2,496,147 + -1.1 2,496,148 - -0.5 2,496,148 - -1.1 2,496,148 - -0.3 2,496,212 + RR42_RS31730 0.15 -1.0 2,496,297 + RR42_RS31730 0.24 -0.1 2,496,297 + RR42_RS31730 0.24 +0.7 2,496,297 + RR42_RS31730 0.24 +0.2 2,496,298 - RR42_RS31730 0.24 -1.3 2,496,298 - RR42_RS31730 0.24 -0.0 2,496,344 - RR42_RS31730 0.29 -0.5 2,496,384 + RR42_RS31730 0.33 -0.1 2,496,384 + RR42_RS31730 0.33 -0.2 2,496,384 + +2.7 2,496,385 - RR42_RS31730 0.33 -0.5 2,496,427 + RR42_RS31730 0.37 -0.4 2,496,444 - RR42_RS31730 0.39 +0.6 2,496,459 + RR42_RS31730 0.41 +0.2 2,496,459 + RR42_RS31730 0.41 +0.1 2,496,459 + RR42_RS31730 0.41 +0.0 2,496,459 + RR42_RS31730 0.41 -0.4 2,496,460 - RR42_RS31730 0.41 +1.1 2,496,460 - RR42_RS31730 0.41 +0.1 2,496,765 + RR42_RS31730 0.73 +0.4 2,496,766 - RR42_RS31730 0.73 +1.0 2,497,101 + RR42_RS31735 0.12 +0.0 2,497,255 - RR42_RS31735 0.32 +0.4 2,497,255 - RR42_RS31735 0.32 +0.4 2,497,791 - +0.4 2,497,849 + RR42_RS31740 0.12 -1.1 2,497,849 + RR42_RS31740 0.12 -1.9 2,497,850 - RR42_RS31740 0.12 +0.9 2,497,850 - RR42_RS31740 0.12 -0.6 2,497,871 - RR42_RS31740 0.15 -2.1 2,497,948 - RR42_RS31740 0.27 +0.1 2,497,948 - RR42_RS31740 0.27 +0.1 2,498,132 - RR42_RS31740 0.54 +0.1 2,498,282 - RR42_RS31740 0.77 -1.0 2,498,282 - RR42_RS31740 0.77 +0.7 2,498,455 - +0.7 2,498,455 - -0.4 2,498,455 - +0.5 2,498,458 + -0.5 2,498,458 + -1.2 2,498,458 + +0.7 2,498,458 + +1.0 2,498,497 - -1.0 2,498,581 - RR42_RS31745 0.17 +0.2 2,498,637 + RR42_RS31745 0.25 +0.5 2,498,637 + RR42_RS31745 0.25 -1.0 2,498,637 + RR42_RS31745 0.25 -0.8 2,498,638 - RR42_RS31745 0.25 -0.7 2,498,638 - RR42_RS31745 0.25 -1.5 2,498,746 - RR42_RS31745 0.39 +0.9 2,498,746 - RR42_RS31745 0.39 -0.0 2,498,746 - RR42_RS31745 0.39 +0.5
Or see this region's nucleotide sequence