Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27115 and RR42_RS27120 overlap by 20 nucleotides RR42_RS27120 and RR42_RS27125 overlap by 4 nucleotides RR42_RS27125 and RR42_RS27130 are separated by 161 nucleotides
RR42_RS27115: RR42_RS27115 - amino acid ABC transporter ATP-binding protein, at 1,435,985 to 1,436,713
_RS27115
RR42_RS27120: RR42_RS27120 - amino acid ABC transporter permease, at 1,436,694 to 1,437,350
_RS27120
RR42_RS27125: RR42_RS27125 - polar amino acid ABC transporter permease, at 1,437,347 to 1,438,012
_RS27125
RR42_RS27130: RR42_RS27130 - amino acid ABC transporter substrate-binding protein, at 1,438,174 to 1,438,956
_RS27130
Position (kb)
1437
1438
1439 Strain fitness (log2 ratio)
-2
-1
0
1 at 1436.536 kb on + strand, within RR42_RS27115 at 1436.569 kb on + strand, within RR42_RS27115 at 1436.954 kb on - strand, within RR42_RS27120 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.421 kb on - strand, within RR42_RS27125 at 1437.834 kb on + strand, within RR42_RS27125 at 1437.835 kb on - strand, within RR42_RS27125 at 1438.005 kb on + strand at 1438.005 kb on + strand at 1438.073 kb on - strand at 1438.073 kb on - strand at 1438.172 kb on + strand at 1438.454 kb on + strand, within RR42_RS27130 at 1438.454 kb on + strand, within RR42_RS27130 at 1438.593 kb on + strand, within RR42_RS27130 at 1438.595 kb on + strand, within RR42_RS27130 at 1438.608 kb on - strand, within RR42_RS27130 at 1438.608 kb on - strand, within RR42_RS27130 at 1438.694 kb on + strand, within RR42_RS27130 at 1438.957 kb on - strand at 1438.957 kb on - strand at 1438.969 kb on + strand at 1438.969 kb on + strand at 1438.970 kb on - strand at 1438.970 kb on - strand at 1439.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 1,436,536 + RR42_RS27115 0.76 +1.3 1,436,569 + RR42_RS27115 0.80 -1.0 1,436,954 - RR42_RS27120 0.40 -0.9 1,437,420 + RR42_RS27125 0.11 -0.8 1,437,420 + RR42_RS27125 0.11 -0.0 1,437,420 + RR42_RS27125 0.11 +0.9 1,437,421 - RR42_RS27125 0.11 -1.6 1,437,834 + RR42_RS27125 0.73 +0.2 1,437,835 - RR42_RS27125 0.73 +0.2 1,438,005 + +0.2 1,438,005 + -0.1 1,438,073 - -0.1 1,438,073 - +0.7 1,438,172 + +1.4 1,438,454 + RR42_RS27130 0.36 +0.8 1,438,454 + RR42_RS27130 0.36 -0.3 1,438,593 + RR42_RS27130 0.54 -0.7 1,438,595 + RR42_RS27130 0.54 -0.2 1,438,608 - RR42_RS27130 0.55 -0.0 1,438,608 - RR42_RS27130 0.55 +0.0 1,438,694 + RR42_RS27130 0.66 -1.6 1,438,957 - -0.3 1,438,957 - +0.2 1,438,969 + +0.8 1,438,969 + -0.4 1,438,970 - +0.3 1,438,970 - +0.6 1,439,007 - -2.0
Or see this region's nucleotide sequence