Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23360 and RR42_RS23365 overlap by 4 nucleotides RR42_RS23365 and RR42_RS23370 are separated by 44 nucleotides RR42_RS23370 and RR42_RS23375 overlap by 8 nucleotides RR42_RS23375 and RR42_RS23380 are separated by 150 nucleotides
RR42_RS23360: RR42_RS23360 - flagellar biosynthesis protein flip, at 570,733 to 571,575
_RS23360
RR42_RS23365: RR42_RS23365 - flagellar biosynthesis protein FliO, at 571,572 to 572,042
_RS23365
RR42_RS23370: RR42_RS23370 - flagellar motor switch protein FliN, at 572,087 to 572,545
_RS23370
RR42_RS23375: RR42_RS23375 - flagellar motor switch protein FliM, at 572,538 to 573,545
_RS23375
RR42_RS23380: RR42_RS23380 - flagellar basal body protein FliL, at 573,696 to 574,181
_RS23380
Position (kb) 572
573
574 Strain fitness (log2 ratio) -2
-1
0
1
2 at 571.800 kb on + strand, within RR42_RS23365 at 571.801 kb on - strand, within RR42_RS23365 at 572.190 kb on + strand, within RR42_RS23370 at 572.303 kb on + strand, within RR42_RS23370 at 572.304 kb on - strand, within RR42_RS23370 at 572.596 kb on - strand at 572.609 kb on - strand at 572.609 kb on - strand at 572.697 kb on + strand, within RR42_RS23375 at 572.698 kb on - strand, within RR42_RS23375 at 572.698 kb on - strand, within RR42_RS23375 at 572.698 kb on - strand, within RR42_RS23375 at 572.731 kb on - strand, within RR42_RS23375 at 572.821 kb on - strand, within RR42_RS23375 at 572.821 kb on - strand, within RR42_RS23375 at 573.002 kb on + strand, within RR42_RS23375 at 573.024 kb on + strand, within RR42_RS23375 at 573.024 kb on + strand, within RR42_RS23375 at 573.024 kb on + strand, within RR42_RS23375 at 573.024 kb on + strand, within RR42_RS23375 at 573.046 kb on + strand, within RR42_RS23375 at 573.046 kb on + strand, within RR42_RS23375 at 573.047 kb on - strand, within RR42_RS23375 at 573.274 kb on + strand, within RR42_RS23375 at 573.275 kb on - strand, within RR42_RS23375 at 573.427 kb on + strand, within RR42_RS23375 at 573.428 kb on - strand, within RR42_RS23375 at 573.428 kb on - strand, within RR42_RS23375 at 573.510 kb on + strand at 573.547 kb on + strand at 573.548 kb on - strand at 573.548 kb on - strand at 573.548 kb on - strand at 573.548 kb on - strand at 573.697 kb on + strand at 573.698 kb on - strand at 573.716 kb on + strand at 573.717 kb on - strand at 573.717 kb on - strand at 573.721 kb on - strand at 573.721 kb on - strand at 573.883 kb on + strand, within RR42_RS23380 at 573.938 kb on + strand, within RR42_RS23380 at 573.980 kb on + strand, within RR42_RS23380 at 573.980 kb on + strand, within RR42_RS23380 at 573.980 kb on + strand, within RR42_RS23380 at 574.172 kb on + strand at 574.173 kb on - strand at 574.193 kb on - strand at 574.193 kb on - strand at 574.306 kb on - strand at 574.319 kb on + strand at 574.319 kb on + strand at 574.319 kb on + strand at 574.319 kb on + strand at 574.320 kb on - strand at 574.320 kb on - strand at 574.320 kb on - strand at 574.320 kb on - strand at 574.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 571,800 + RR42_RS23365 0.48 -0.3 571,801 - RR42_RS23365 0.49 -0.2 572,190 + RR42_RS23370 0.22 -0.3 572,303 + RR42_RS23370 0.47 -1.8 572,304 - RR42_RS23370 0.47 -0.2 572,596 - +1.4 572,609 - +0.5 572,609 - +1.2 572,697 + RR42_RS23375 0.16 +0.8 572,698 - RR42_RS23375 0.16 +0.7 572,698 - RR42_RS23375 0.16 +1.0 572,698 - RR42_RS23375 0.16 +0.7 572,731 - RR42_RS23375 0.19 +0.5 572,821 - RR42_RS23375 0.28 +2.2 572,821 - RR42_RS23375 0.28 +0.7 573,002 + RR42_RS23375 0.46 -0.7 573,024 + RR42_RS23375 0.48 +0.0 573,024 + RR42_RS23375 0.48 -0.6 573,024 + RR42_RS23375 0.48 -0.2 573,024 + RR42_RS23375 0.48 +0.0 573,046 + RR42_RS23375 0.50 +0.0 573,046 + RR42_RS23375 0.50 +0.5 573,047 - RR42_RS23375 0.50 +0.4 573,274 + RR42_RS23375 0.73 +0.3 573,275 - RR42_RS23375 0.73 +0.2 573,427 + RR42_RS23375 0.88 -0.1 573,428 - RR42_RS23375 0.88 -0.6 573,428 - RR42_RS23375 0.88 -1.5 573,510 + -0.6 573,547 + +0.9 573,548 - +0.1 573,548 - -0.2 573,548 - -0.4 573,548 - +0.5 573,697 + -1.7 573,698 - +0.3 573,716 + -2.0 573,717 - +0.8 573,717 - +2.1 573,721 - +0.9 573,721 - +1.1 573,883 + RR42_RS23380 0.38 +0.5 573,938 + RR42_RS23380 0.50 -0.7 573,980 + RR42_RS23380 0.58 -0.1 573,980 + RR42_RS23380 0.58 -0.1 573,980 + RR42_RS23380 0.58 -1.3 574,172 + -0.1 574,173 - +0.2 574,193 - +0.2 574,193 - +1.3 574,306 - +0.7 574,319 + -0.1 574,319 + +0.1 574,319 + -1.9 574,319 + +0.3 574,320 - -0.9 574,320 - +0.9 574,320 - +2.1 574,320 - -0.2 574,522 - +1.2
Or see this region's nucleotide sequence