Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21210 and RR42_RS21215 are separated by 230 nucleotides RR42_RS21215 and RR42_RS21220 are separated by 18 nucleotides RR42_RS21220 and RR42_RS21225 are separated by 19 nucleotides RR42_RS21225 and RR42_RS21230 are separated by 513 nucleotides
RR42_RS21210: RR42_RS21210 - hypothetical protein, at 83,457 to 84,767
_RS21210
RR42_RS21215: RR42_RS21215 - membrane protein, at 84,998 to 85,318
_RS21215
RR42_RS21220: RR42_RS21220 - iron transporter FeoB, at 85,337 to 87,208
_RS21220
RR42_RS21225: RR42_RS21225 - iron transporter FeoA, at 87,228 to 87,533
_RS21225
RR42_RS21230: RR42_RS21230 - ABC transporter substrate-binding protein, at 88,047 to 89,039
_RS21230
Position (kb) 85
86
87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 84.433 kb on + strand, within RR42_RS21210 at 84.434 kb on - strand, within RR42_RS21210 at 84.492 kb on + strand, within RR42_RS21210 at 84.492 kb on + strand, within RR42_RS21210 at 84.492 kb on + strand, within RR42_RS21210 at 84.493 kb on - strand, within RR42_RS21210 at 84.493 kb on - strand, within RR42_RS21210 at 84.493 kb on - strand, within RR42_RS21210 at 84.586 kb on - strand, within RR42_RS21210 at 84.629 kb on - strand, within RR42_RS21210 at 85.984 kb on - strand, within RR42_RS21220 at 86.067 kb on + strand, within RR42_RS21220 at 86.992 kb on - strand, within RR42_RS21220 at 87.079 kb on - strand at 87.209 kb on - strand at 87.209 kb on - strand at 87.209 kb on - strand at 87.597 kb on - strand at 87.619 kb on - strand at 87.989 kb on - strand at 88.048 kb on + strand at 88.049 kb on - strand at 88.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 84,433 + RR42_RS21210 0.74 +0.2 84,434 - RR42_RS21210 0.75 +0.3 84,492 + RR42_RS21210 0.79 +0.5 84,492 + RR42_RS21210 0.79 +0.5 84,492 + RR42_RS21210 0.79 +0.0 84,493 - RR42_RS21210 0.79 -0.4 84,493 - RR42_RS21210 0.79 +1.0 84,493 - RR42_RS21210 0.79 -0.8 84,586 - RR42_RS21210 0.86 -1.1 84,629 - RR42_RS21210 0.89 -1.7 85,984 - RR42_RS21220 0.35 -0.8 86,067 + RR42_RS21220 0.39 -2.8 86,992 - RR42_RS21220 0.88 -1.0 87,079 - -1.0 87,209 - -1.4 87,209 - -1.5 87,209 - -0.1 87,597 - -2.7 87,619 - +0.7 87,989 - -1.0 88,048 + +0.0 88,049 - -0.9 88,049 - -0.8
Or see this region's nucleotide sequence