Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16680 and RR42_RS16685 are separated by 199 nucleotides RR42_RS16685 and RR42_RS16690 are separated by 335 nucleotides RR42_RS16690 and RR42_RS16695 are separated by 87 nucleotides RR42_RS16695 and RR42_RS16700 are separated by 291 nucleotides
RR42_RS16680: RR42_RS16680 - hypothetical protein, at 3,613,227 to 3,613,589
_RS16680
RR42_RS16685: RR42_RS16685 - tRNA-Leu, at 3,613,789 to 3,613,875
_RS16685
RR42_RS16690: RR42_RS16690 - RNA signal recognition particle 4.5S RNA, at 3,614,211 to 3,614,561
_RS16690
RR42_RS16695: RR42_RS16695 - dehydrogenase, at 3,614,649 to 3,615,086
_RS16695
RR42_RS16700: RR42_RS16700 - ABC transporter substrate-binding protein, at 3,615,378 to 3,616,076
_RS16700
Position (kb)
3614
3615 Strain fitness (log2 ratio)
-2
-1
0
1 at 3613.382 kb on - strand, within RR42_RS16680 at 3613.382 kb on - strand, within RR42_RS16680 at 3613.494 kb on + strand, within RR42_RS16680 at 3613.495 kb on - strand, within RR42_RS16680 at 3613.649 kb on + strand at 3613.649 kb on + strand at 3613.649 kb on + strand at 3613.668 kb on + strand at 3613.669 kb on - strand at 3613.833 kb on + strand, within RR42_RS16685 at 3613.833 kb on + strand, within RR42_RS16685 at 3613.855 kb on + strand, within RR42_RS16685 at 3613.876 kb on + strand at 3613.948 kb on + strand at 3614.054 kb on + strand at 3614.055 kb on - strand at 3614.055 kb on - strand at 3614.104 kb on + strand at 3614.105 kb on - strand at 3614.105 kb on - strand at 3614.217 kb on + strand at 3614.217 kb on + strand at 3614.217 kb on + strand at 3614.217 kb on + strand at 3614.217 kb on + strand at 3614.218 kb on - strand at 3614.218 kb on - strand at 3614.218 kb on - strand at 3614.218 kb on - strand at 3614.265 kb on + strand, within RR42_RS16690 at 3614.265 kb on + strand, within RR42_RS16690 at 3614.266 kb on - strand, within RR42_RS16690 at 3615.014 kb on + strand, within RR42_RS16695 at 3615.015 kb on - strand, within RR42_RS16695 at 3615.109 kb on + strand at 3615.159 kb on + strand at 3615.160 kb on - strand at 3615.160 kb on - strand at 3615.160 kb on - strand at 3615.162 kb on + strand at 3615.205 kb on - strand at 3615.205 kb on - strand at 3615.306 kb on - strand at 3615.316 kb on + strand at 3615.316 kb on + strand at 3615.317 kb on - strand at 3615.330 kb on + strand at 3615.350 kb on + strand at 3615.367 kb on + strand at 3615.367 kb on + strand at 3615.508 kb on - strand, within RR42_RS16700
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 3,613,382 - RR42_RS16680 0.43 +0.3 3,613,382 - RR42_RS16680 0.43 -0.4 3,613,494 + RR42_RS16680 0.74 +0.2 3,613,495 - RR42_RS16680 0.74 -0.2 3,613,649 + +1.6 3,613,649 + +0.9 3,613,649 + +0.6 3,613,668 + -0.6 3,613,669 - +0.4 3,613,833 + RR42_RS16685 0.51 -0.2 3,613,833 + RR42_RS16685 0.51 +0.0 3,613,855 + RR42_RS16685 0.76 +0.7 3,613,876 + -0.1 3,613,948 + -0.3 3,614,054 + -2.1 3,614,055 - +0.3 3,614,055 - +0.8 3,614,104 + -0.6 3,614,105 - -0.5 3,614,105 - +0.2 3,614,217 + +0.9 3,614,217 + -0.0 3,614,217 + +1.1 3,614,217 + -0.0 3,614,217 + +1.3 3,614,218 - +1.3 3,614,218 - -1.8 3,614,218 - +0.7 3,614,218 - +0.4 3,614,265 + RR42_RS16690 0.15 -0.8 3,614,265 + RR42_RS16690 0.15 +0.1 3,614,266 - RR42_RS16690 0.16 -0.2 3,615,014 + RR42_RS16695 0.83 -0.1 3,615,015 - RR42_RS16695 0.84 +0.1 3,615,109 + +0.3 3,615,159 + +0.4 3,615,160 - -0.2 3,615,160 - -0.1 3,615,160 - +0.6 3,615,162 + -1.8 3,615,205 - +0.6 3,615,205 - +0.5 3,615,306 - -0.8 3,615,316 + -1.1 3,615,316 + +0.1 3,615,317 - +0.0 3,615,330 + +0.6 3,615,350 + +1.6 3,615,367 + -0.3 3,615,367 + -0.2 3,615,508 - RR42_RS16700 0.19 +1.3
Or see this region's nucleotide sequence