Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15215

Experiment: R2A with Nickel (II) chloride 0.35 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS15205 and RR42_RS15210 are separated by 4 nucleotidesRR42_RS15210 and RR42_RS15215 are separated by 53 nucleotidesRR42_RS15215 and RR42_RS15220 are separated by 214 nucleotides RR42_RS15205: RR42_RS15205 - pyridoxamine 5'-phosphate oxidase, at 3,293,958 to 3,294,578 _RS15205 RR42_RS15210: RR42_RS15210 - negative transcriptional regulator, at 3,294,583 to 3,295,200 _RS15210 RR42_RS15215: RR42_RS15215 - carnitine dehydratase, at 3,295,254 to 3,296,693 _RS15215 RR42_RS15220: RR42_RS15220 - glutaminyl-tRNA synthetase, at 3,296,908 to 3,298,686 _RS15220 Position (kb) 3295 3296 3297Strain fitness (log2 ratio) -1 0 1at 3294.573 kb on + strandat 3294.573 kb on + strandat 3294.573 kb on + strandat 3294.574 kb on - strandat 3294.574 kb on - strandat 3294.574 kb on - strandat 3294.586 kb on + strandat 3294.586 kb on + strandat 3294.586 kb on + strandat 3294.586 kb on + strandat 3294.586 kb on + strandat 3294.586 kb on + strandat 3294.586 kb on + strandat 3294.586 kb on + strandat 3294.587 kb on - strandat 3294.587 kb on - strandat 3294.587 kb on - strandat 3294.587 kb on - strandat 3294.587 kb on - strandat 3294.753 kb on + strand, within RR42_RS15210at 3294.754 kb on - strand, within RR42_RS15210at 3294.832 kb on + strand, within RR42_RS15210at 3294.832 kb on + strand, within RR42_RS15210at 3294.832 kb on + strand, within RR42_RS15210at 3294.833 kb on - strand, within RR42_RS15210at 3294.851 kb on - strand, within RR42_RS15210at 3294.883 kb on + strand, within RR42_RS15210at 3294.883 kb on + strand, within RR42_RS15210at 3294.884 kb on - strand, within RR42_RS15210at 3294.901 kb on + strand, within RR42_RS15210at 3294.901 kb on + strand, within RR42_RS15210at 3294.901 kb on + strand, within RR42_RS15210at 3294.901 kb on + strand, within RR42_RS15210at 3295.727 kb on - strand, within RR42_RS15215at 3295.727 kb on - strand, within RR42_RS15215at 3295.727 kb on - strand, within RR42_RS15215at 3295.799 kb on - strand, within RR42_RS15215at 3296.329 kb on - strand, within RR42_RS15215at 3296.410 kb on + strand, within RR42_RS15215at 3296.678 kb on + strandat 3296.678 kb on + strandat 3296.734 kb on + strandat 3296.735 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM
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3,294,573 + -0.5
3,294,573 + -0.3
3,294,573 + +0.5
3,294,574 - -0.1
3,294,574 - +0.2
3,294,574 - +0.1
3,294,586 + +0.6
3,294,586 + -0.3
3,294,586 + +0.5
3,294,586 + +0.1
3,294,586 + -1.5
3,294,586 + +1.4
3,294,586 + +0.0
3,294,586 + +0.0
3,294,587 - +0.6
3,294,587 - -0.6
3,294,587 - +0.2
3,294,587 - +0.2
3,294,587 - +0.1
3,294,753 + RR42_RS15210 0.28 -0.0
3,294,754 - RR42_RS15210 0.28 -1.5
3,294,832 + RR42_RS15210 0.40 +0.5
3,294,832 + RR42_RS15210 0.40 -0.3
3,294,832 + RR42_RS15210 0.40 -0.0
3,294,833 - RR42_RS15210 0.40 +0.4
3,294,851 - RR42_RS15210 0.43 -0.5
3,294,883 + RR42_RS15210 0.49 -0.7
3,294,883 + RR42_RS15210 0.49 -0.8
3,294,884 - RR42_RS15210 0.49 +0.3
3,294,901 + RR42_RS15210 0.51 +0.6
3,294,901 + RR42_RS15210 0.51 +0.3
3,294,901 + RR42_RS15210 0.51 -0.1
3,294,901 + RR42_RS15210 0.51 +0.6
3,295,727 - RR42_RS15215 0.33 -1.4
3,295,727 - RR42_RS15215 0.33 +0.4
3,295,727 - RR42_RS15215 0.33 +0.5
3,295,799 - RR42_RS15215 0.38 +0.9
3,296,329 - RR42_RS15215 0.75 -0.2
3,296,410 + RR42_RS15215 0.80 +0.6
3,296,678 + -0.7
3,296,678 + -0.2
3,296,734 + +0.1
3,296,735 - -0.3

Or see this region's nucleotide sequence